Early disruption of the actin cytoskeleton in cultured cerebellar granule neurons exposed to 3-morpholinosydnonimine-oxidative stress is linked to alterations of the cytosolic calcium concentration

Cell Calcium ◽  
2011 ◽  
Vol 49 (3) ◽  
pp. 174-183 ◽  
Author(s):  
Teresa Tiago ◽  
Dorinda Marques-da-Silva ◽  
Alejandro K. Samhan-Arias ◽  
Manuel Aureliano ◽  
Carlos Gutierrez-Merino
Keyword(s):  
Oxidative Stress ◽  
Actin Cytoskeleton ◽  
Calcium Concentration ◽  
Cytosolic Calcium ◽  
Cerebellar Granule Neurons ◽  
Granule Neurons ◽  
Cerebellar Granule ◽  
Cytosolic Calcium Concentration

scholarly journals Ethanol exposure of neonatal rats does not increase biomarkers of oxidative stress in isolated cerebellar granule neurons

Alcohol ◽  
2008 ◽  
Vol 42 (1) ◽  
pp. 29-36 ◽  
Author(s):  
Cynthia J.M. Kane ◽  
Jason Y. Chang ◽  
Paula K. Roberson ◽  
Tarun K. Garg ◽  
Lihong Han
Keyword(s):  
Oxidative Stress ◽  
Ethanol Exposure ◽  
Cerebellar Granule Neurons ◽  
Granule Neurons ◽  
Neonatal Rats ◽  
Cerebellar Granule ◽  
Biomarkers Of Oxidative Stress

scholarly journals The Protection of Hepatocyte Cells from the Effects of Oxidative Stress by Treatment with Vitamin E in Conjunction with DTT

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Jen-Hsiang Tsai ◽  
Haw-Wen Chen ◽  
Yi-Wan Chen ◽  
Jer-Yuh Liu ◽  
Chong-Kuei Lii
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Membrane Protein ◽  
Calcium Concentration ◽  
Cytosolic Calcium ◽  
Butyl Hydroperoxide ◽  
Cytosolic Calcium Concentration ◽  
Protein Thiols ◽  
Bleb Formation ◽  
Hepatocyte Cells

We investigated the effect of vitamin E on membrane protein thiols under oxidative stress, which we induced by treating hepatocytes withtert-butyl hydroperoxide (TBH) for 60 mins. Those cells which we pretreated with vitamin E formed fewer blebs (22.3% compared to 60.0% in nonvitamin E-treated cells) and maintained cytosolic calcium concentration and the number of membrane protein thiols instead of showing the usual symptoms in cells undergoing oxidative stress. Dithiothreitol (DTT) also commonly reduces bleb formation in hepatocytes affected by TBH. However, our experiments clearly demonstrate that DTT does not prevent the changes in cytosolic calcium and membrane protein thiols in the blebbing cells. Consequently, we decided to pretreat cells with both DTT and vitamin E and found that the influence of TBH was entirely prevented. These findings may provide us with a new aspect for investigating the mechanism of bleb formation under oxidative stress.

scholarly journals Protective effects of Sanguisorba minor and Ferulago angulata total extracts against beta-amyloid induced cytotoxicity and oxidative stress in cultured cerebellar granule neurons

2019 ◽  
Vol 8 (3) ◽  
pp. 248-255 ◽  
Author(s):  
Sholeh Akbari ◽  
Maliheh Soodi ◽  
Homa Hajimehdipoor ◽  
Nasim Ataei
Keyword(s):  
Oxidative Stress ◽  
Ache Activity ◽  
Neurodegenerative Disorder ◽  
Beta Amyloid ◽  
Cerebellar Granule Neurons ◽  
Cultured Neurons ◽  
Granule Neurons ◽  
Cerebellar Granule ◽  
Sanguisorba Minor ◽  
And Oxidative Stress

Introduction: Alzheimer’s disease (AD) is an age-dependent neurodegenerative disorder and major cause of mortality in the elderly. AD has a complex pathophysiology and needs new multi-targeted compounds to halt the disease progression through several mechanisms. Medicinal plants contain various compounds with heterogeneous pharmacological effects, therefore are a good source. The aim of this study was to evaluate the protective effect of total extracts of Sanguisorba minor and Ferulago angulata on beta-amyloid (Aβ)-induced toxicity in primary neural cell culture.Methods: Cerebellar granule neurons (CGNs) were cultured according to standard protocols. The cultured neurons were incubated with Aβ alone or in combination with different concentrations of extracts for 24 hours. Cell viability was measured by methylthiazolyldiphenyl-tetrazolium (MTT) assay. In addition acetylcholinesterase (AChE) activity and oxidative stress markers were measured after incubation. Also, the effects of different concentrations of the extracts on AChE activity of the cultured neurons were investigated. For measuring the acute toxicity of the extract, LD50 was estimated by limit test.Results: Both extracts could protect CGNs against Aβ-induced cell death. Aβ-induced oxidative stress and increase of AChE activity were ameliorated by both extracts. S. minor extract dose-dependently reduced AChE activity in cultured CGNs. LD50 of both extracts was estimated above 2000 mg/kg and considered as safe.Conclusion: Both studied extracts protected CGNs against Aβ-induced toxicity by ameliorating oxidative stress mechanism. According to these results, these extracts are recommended for further investigation in AD treatment.

scholarly journals Mitochondrial ATP-Sensitive Potassium Channel Activation Protects Cerebellar Granule Neurons From Apoptosis Induced by Oxidative Stress

Stroke ◽  
2003 ◽  
Vol 34 (7) ◽  
pp. 1796-1802 ◽  
Author(s):  
Yasushi Teshima ◽  
Masaharu Akao ◽  
Ronald A. Li ◽  
Tae H. Chong ◽  
William A. Baumgartner ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Potassium Channel ◽  
Cerebellar Granule Neurons ◽  
Granule Neurons ◽  
Channel Activation ◽  
Cerebellar Granule
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