Don’t we want to know whether zinc accumulation contributes to the calcium transient measured with these ‘calcium’ fluorophores?

Cell Calcium ◽  
2007 ◽  
Vol 42 (3) ◽  
pp. 343-344 ◽  
Author(s):  
Christian J. Stork ◽  
Yang V Li
Keyword(s):  
Calcium Transient ◽  
Zinc Accumulation

Increased zinc accumulation in mineralized osteosarcoma tissue

2016 ◽  
Author(s):  
Mirjam Rauwolf ◽  
Bernhard Pemmer ◽  
Andreas Roschger ◽  
Anna Turyanskaya ◽  
Rolf Simon ◽  
...  
Keyword(s):  
Zinc Accumulation

scholarly journals EDDS and EDTA-enhanced zinc accumulation by solanum nigrum inoculated with arbuscular mycorrhizal fungi grown in contaminated soil

Chemosphere ◽  
2008 ◽  
Vol 70 (6) ◽  
pp. 1002-1014 ◽  
Author(s):  
Ana P.G.C. Marques ◽  
Rui S. Oliveira ◽  
Kalina A. Samardjieva ◽  
José Pissarra ◽  
António O.S.S. Rangel ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Contaminated Soil ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Solanum Nigrum ◽  
Zinc Accumulation

Zinc Accumulation in Monstera Deliciosa Near Brisbane.

1991 ◽  
Vol 20 (4) ◽  
pp. 125 ◽  
Author(s):  
GF Haydon ◽  
DE Shaw
Keyword(s):  
Zinc Accumulation ◽  
Monstera Deliciosa

Characterization of positive inotropic effect of colforsin dapropate hydrochloride, a water-soluble forskolin derivative, in isolated adult rat cardiomyocytes

1999 ◽  
Vol 77 (4) ◽  
pp. 225-234 ◽  
Author(s):  
Rikako Miyake ◽  
Hiroyuki Yoshida ◽  
Kouichi Tanonaka ◽  
Yuki Miyamoto ◽  
Hideharu Hayashi ◽  
...  
Keyword(s):  
Calcium Transient ◽  
Water Soluble ◽  
Inotropic Action ◽  
Shortening Velocity ◽  
Adult Rats ◽  
Camp Content ◽  
Rat Cardiomyocytes ◽  
Positive Inotropic Action ◽  
Time To Peak ◽  
Rate Of Increase

The present study was undertaken to characterize the positive inotropic action of colforsin dapropate hydrochloride (NKH477), a novel water-soluble forskolin derivative, on isolated cardiomyocytes of adult rats. Simultaneous measurements of cellular contraction and intracellular calcium concentration ([Ca2+]i) were carried out. The effects of isoprenaline and ouabain on these parameters were also determined for comparison. The contraction and maximum [Ca2+]i of NKH477-, isoprenaline-, or ouabain-treated cells were increased concentration dependently. Peak shortening of NKH477-treated cells was positively correlated with the shortening velocity and inversely with the time to peak shortening. Maximum, but not minimum, [Ca2+]i in NKH477-treated cells was correlated with the rate of increase in [Ca2+]i and inversely with the time to maximum [Ca2+]i. Similar results were obtained with isoprenaline. In contrast, ouabain increased both maximum and minimum [Ca2+]i. Treatment with either NKH477 or isoprenaline increased cellular cAMP content, but treatment with ouabain did not. These results suggest that the positive inotropic action of NKH477 is associated with an increase in [Ca2+]i and acceleration of its kinetics.Key words: adenylate cyclase, calcium transient, colforsin dapropate, isoprenaline, ouabain.

scholarly journals Direct visualization of mitochondrial zinc accumulation reveals uniporter-dependent and -independent transport mechanisms

2005 ◽  
Vol 93 (5) ◽  
pp. 1242-1250 ◽  
Author(s):  
Latha M. Malaiyandi ◽  
Olga Vergun ◽  
Kirk E. Dineley ◽  
Ian J. Reynolds
Keyword(s):  
Direct Visualization ◽  
Transport Mechanisms ◽  
Zinc Accumulation

Abstract P498: Polycystin-1 Regulates Excitation-contraction Coupling In Atrial Cardiomyocytes

2021 ◽  
Vol 129 (Suppl_1) ◽  
Author(s):  
Troy Hendrickson ◽  
William Perez ◽  
Vincent Provasek ◽  
Francisco J Altamirano
Keyword(s):  
Electrical Stimulation ◽  
Action Potentials ◽  
Primary Cilia ◽  
Calcium Influx ◽  
Calcium Transient ◽  
Calcium Handling ◽  
Excitation Contraction Coupling ◽  
Contraction Coupling ◽  
Atrial Cardiomyocytes ◽  
Atrial Excitation

Patients with Autosomal Dominant Polycystic Kidney disease (ADPKD) have multiple cardiovascular manifestations, including increased susceptibility to arrhythmias. Mutations in polycystin-1 (PC1) encoding gene accounts for 85% cases of ADPKD, whereas mutations in polycystin-2 (PC2) only accounts for 15%. In kidney cells, PC1 interacts with PC2 to form a protein complex at the primary cilia to regulate calcium influx via PC2. However, cardiomyocytes are non-ciliated cells and the role of both PC1 and PC2 in atrial cardiomyocytes remains unknown. We have previously demonstrated that PC1 regulates action potentials and calcium handling to fine-tune ventricular cardiomyocyte contraction. Here, we hypothesize that PC1 regulates action potentials and calcium handling in atrial cardiomyocytes independent of PC2 actions. To test this hypothesis, we differentiated human induced pluripotent stem cells (iPSC) into atrial cardiomyocytes (iPSC-aCM) using previously published protocols. To determine the contribution of PC1/PC2 in atrial excitation-contraction coupling, protein expression was knocked down utilizing specific siRNA constructs, for each protein, or a universal control siRNA transfected using lipofectamine RNAiMAX. We measured action potentials using the potentiometric dye FluoVolt and intracellular calcium with Fura-2 AM or Fluo-4. Changes in fluorescence were monitored using a multiwavelength IonOptix system. iPSC-aCM were paced at 2 Hz to synchronize the beating pattern using field electrical stimulation. Our data shows that PC1 ablation significantly decreased action potential duration at 50% and 80% of repolarization, by 24% and 23%, respectively. Moreover, we observed that PC1 knockdown significantly reduced calcium transient amplitude elicited by field electrical stimulation without changes in calcium transient decay. Interestingly, PC2 knockdown did not modify calcium transients in atrial cardiomyocytes (iPSC-aCM). Our data suggest that PC1 regulates atrial excitation-contraction coupling independent of PC2 actions. This study warrants further investigation into atrial dysfunction in ADPKD patients with PC1 mutations.

Abstract 37: Nrip Deficiency Leads to Dilated Cardiomyopathy

2015 ◽  
Vol 117 (suppl_1) ◽  
Author(s):  
Show-Li Chen
Keyword(s):  
Calcium Transient ◽  
Receptor Interaction ◽  
Cell Width ◽  
Interacting Protein ◽  
Cardiac Tissues ◽  
Calmodulin Binding ◽  
Calcineurin Phosphatase ◽  
And Function

Previously, we demonstrate a gene, nuclear receptor interaction protein (NRIP, also named DCAF6 or IQWD1) as a Ca2+- dependent calmodulin binding protein that can activate calcineurin phosphatase activity. Here, we found that α-actinin-2 (ACTN2), is one of NRIP-interacting proteins from the yeast two-hybrid system using NRIP as a prey. We further confirmed the direct bound between NRIP and ACTN2 using in vitro protein-protein interaction and in vivo co-immunoprecipitation assays. To further map the binding domain of each protein, the results showed the IQ domain of NRIP responsible for ACTN2 binding, and EF hand motif of ACTN2 responsible for NRIP bound. Due to ACTN2 is a biomarker of muscular Z-disc complex; we found the co-localization of NRIP and ACTN2 in cardiac tissues by immunofluorescence assays. Taken together, NRIP is a novel ACTN2-interacting protein. To investigate insights into in vivo function of NRIP, we generated conventional NRIP-null mice. The H&E staining results are shown in the hearts of NRIP KO mice are enlarged and dilated and the cell width of NRIP KO cardiomyocyte is increased. The EM of NRIP KO heart muscles reveal the reduction of I-band width and extension length of Z-disc in sarcomere structure; and the echocardiography shows the diminished fractional shortening in heart functions. Additionally, the calcium transient and sarcomere contraction length in cardiomyocytes of NRIP KO is weaker and shorter than wt; respectively. In conclusion, NRIP is a novel Z-disc protein and has function for maintenance of sarcomere integrity structure and function for calcium transient and muscle contraction.

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