Pharmacological modulation of ion channels and transporters

Cell Calcium ◽  
2004 ◽  
Vol 35 (6) ◽  
pp. 575-582 ◽  
Author(s):  
Ursula Ravens ◽  
Erich Wettwer ◽  
Ottó Hála
Keyword(s):  
Ion Channels ◽  
Pharmacological Modulation

scholarly journals Plasma membrane ion channels and epithelial to mesenchymal transition in cancer cells

2016 ◽  
Vol 23 (11) ◽  
pp. R517-R525 ◽  
Author(s):  
Iman Azimi ◽  
Gregory R Monteith
Keyword(s):  
Plasma Membrane ◽  
Ion Channels ◽  
Ion Channel ◽  
Cancer Cells ◽  
Epithelial To Mesenchymal Transition ◽  
Therapeutic Resistance ◽  
Therapeutic Approaches ◽  
Mesenchymal Transition ◽  
Pharmacological Modulation ◽  
Channel Expression

A variety of studies have suggested that epithelial to mesenchymal transition (EMT) may be important in the progression of cancer in patients through metastasis and/or therapeutic resistance. A number of pathways have been investigated in EMT in cancer cells. Recently, changes in plasma membrane ion channel expression as a consequence of EMT have been reported. Other studies have identified specific ion channels able to regulate aspects of EMT induction. The utility of plasma membrane ion channels as targets for pharmacological modulation make them attractive for therapeutic approaches to target EMT. In this review, we provide an overview of some of the key plasma membrane ion channel types and highlight some of the studies that are beginning to define changes in plasma membrane ion channels as a consequence of EMT and also their possible roles in EMT induction.

scholarly journals High-throughput characterization of 309 photocrosslinker-bearing ASIC1a variants maps residues critical for channel function and pharmacology

2020 ◽  
Author(s):  
Nina Braun ◽  
Søren Friis ◽  
Christian Ihling ◽  
Andrea Sinz ◽  
Jacob Andersen ◽  
...  
Keyword(s):  
Ion Channels ◽  
High Throughput ◽  
Mammalian Cells ◽  
Great Precision ◽  
Pharmacological Modulation ◽  
Current Decay ◽  
Human Acid ◽  
Automated Patch Clamp ◽  
Insight Into

AbstractIncorporation of non-canonical amino acids (ncAAs) can endow proteins with novel functionalities, such as crosslinking or fluorescence. In ion channels, the function of these variants can be studied with great precision using standard electrophysiology, but this approach is typically labor intensive and low throughput. Here, we establish a high-throughput protocol to conduct functional and pharmacological investigations of ncAA-containing hASIC1a (human acid-sensing ion channel 1a) variants in transiently transfected mammalian cells. We introduce three different photocrosslinking ncAAs into 103 positions and assess the function of the resulting 309 variants with automated patch-clamp (APC). We demonstrate that the approach is efficient and versatile, as it is amenable to assessing even complex pharmacological modulation by peptides. The data show that the acidic pocket is a major determinant for current decay and live-cell crosslinking provides insight into the hASIC1a-psalmotoxin-1 interaction. Overall, this protocol will enable future APC-based studies of ncAA-containing ion channels in mammalian cells.

scholarly journals Pharmacological Modulation of Ion Channels for the Treatment of Cystic Fibrosis

2021 ◽  
Vol Volume 13 ◽  
pp. 693-723
Author(s):  
Madalena C Pinto ◽  
Iris AL Silva ◽  
Miriam F Figueira ◽  
Margarida D Amaral ◽  
Miquéias Lopes-Pacheco
Keyword(s):  
Cystic Fibrosis ◽  
Ion Channels ◽  
Pharmacological Modulation

scholarly journals Pharmacological modulation of mitochondrial ion channels

2019 ◽  
Vol 176 (22) ◽  
pp. 4258-4283 ◽  
Author(s):  
Luigi Leanza ◽  
Vanessa Checchetto ◽  
Lucia Biasutto ◽  
Andrea Rossa ◽  
Roberto Costa ◽  
...  
Keyword(s):  
Ion Channels ◽  
Pharmacological Modulation
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