Gene silencing of selected calcium-signalling molecules in a Drosophila cell line using double-stranded RNA interference

Cell Calcium ◽  
2004 ◽  
Vol 35 (2) ◽  
pp. 131-139 ◽  
Author(s):  
Valerie Raymond-Delpech ◽  
Paula R Towers ◽  
David B Sattelle
Keyword(s):  
Rna Interference ◽  
Gene Silencing ◽  
Cell Line ◽  
Calcium Signalling ◽  
Drosophila Cell ◽  
Double Stranded Rna ◽  
Signalling Molecules ◽  
Drosophila Cell Line

scholarly journals Viroids as a Tool to Study RNA-Directed DNA Methylation in Plants

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1187
Author(s):  
Michael Wassenegger ◽  
Athanasios Dalakouras
Keyword(s):  
Dna Methylation ◽  
Rna Interference ◽  
Gene Silencing ◽  
Plant Cells ◽  
Transcriptional Gene Silencing ◽  
Rnai Machinery ◽  
Double Stranded Rna ◽  
Post Transcriptional Gene Silencing ◽  
Cumulative Amount

Viroids are plant pathogenic, circular, non-coding, single-stranded RNAs (ssRNAs). Members of the Pospiviroidae family replicate in the nucleus of plant cells through double-stranded RNA (dsRNA) intermediates, thus triggering the host’s RNA interference (RNAi) machinery. In plants, the two RNAi pillars are Post-Transcriptional Gene Silencing (PTGS) and RNA-directed DNA Methylation (RdDM), and the latter has the potential to trigger Transcriptional Gene Silencing (TGS). Over the last three decades, the employment of viroid-based systems has immensely contributed to our understanding of both of these RNAi facets. In this review, we highlight the role of Pospiviroidae in the discovery of RdDM, expound the gradual elucidation through the years of the diverse array of RdDM’s mechanistic details and propose a revised RdDM model based on the cumulative amount of evidence from viroid and non-viroid systems.

20-Hydroxyecdysone-induced differentiation and apoptosis in the Drosophila cell line, l(2)mbn

2000 ◽  
Vol 32 (6) ◽  
pp. 464-477 ◽  
Author(s):  
C. Ress ◽  
M. Holtmann ◽  
U. Maas ◽  
J. Sofsky ◽  
A. Dorn
Keyword(s):  
Cell Line ◽  
Induced Differentiation ◽  
Drosophila Cell ◽  
Drosophila Cell Line
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