scholarly journals A picket fence function for adherens junctions in epithelial cell polarity

2021 ◽  
pp. 203719
Author(s):  
Teresa Bonello ◽  
Mario Aguilar-Aragon ◽  
Alexander Tournier ◽  
Barry J. Thompson
Keyword(s):  
Epithelial Cell ◽  
Cell Polarity ◽  
Adherens Junctions ◽  
Epithelial Cell Polarity ◽  
Picket Fence ◽  
Fence Function

scholarly journals A Novel Monoclonal Antibody Against the Second Extracellular Loop of Occludin Disrupts Epithelial Cell Polarity

2007 ◽  
Vol 55 (7) ◽  
pp. 735-744 ◽  
Author(s):  
Yuichi Tokunaga ◽  
Takashi Kojima ◽  
Makoto Osanai ◽  
Masaki Murata ◽  
Hideki Chiba ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Polarity ◽  
Basolateral Membrane ◽  
Epithelial Cell Line ◽  
Intestinal Epithelial ◽  
Extracellular Loop ◽  
T84 Cells ◽  
Epithelial Cell Polarity ◽  
Apical Membranes ◽  
Fence Function

The tight junction (TJ) regulates epithelial cell polarity and paracellular permeability. In the present study, to investigate whether the second extracellular loop of occludin affects the localization of carcinoembryonic antigen (CEA) and CD26 expressed on apical membranes, and the fence function of the TJ, the human intestinal epithelial cell line T84 was treated with the monoclonal anti-occludin antibody (MAb) 1H8, corresponding to the second extracellular loop of occludin. In T84 cells treated with MAb 1H8, occludin disappeared, and CEA and CD26 were observed to diffuse from the apical membrane to the basolateral membrane. Furthermore, a decrease in the fence function of TJ was observed without changes in the TJ strands and barrier function. When T84 cells precultured in low calcium (Ca) medium were recultured in normal Ca medium in the presence of MAb 1H8, recruitment of occludin to the apical-most membranes and recovery in distribution of CEA and CD26 were markedly retarded compared with the control. These results suggested that MAb 1H8 against the second extracellular loop of occludin selectively affected formation of the apical/basolateral intramembrane diffusion barrier and that the second extracellular loop of occludin plays a crucial role in the maintenance of epithelial cell polarity by the TJ.

scholarly journals SEGGA: a toolset for rapid automated analysis of epithelial cell polarity and dynamics

Development ◽  
2017 ◽  
Vol 144 (9) ◽  
pp. 1725-1734 ◽  
Author(s):  
Dene L. Farrell ◽  
Ori Weitz ◽  
Marcelo O. Magnasco ◽  
Jennifer A. Zallen
Keyword(s):  
Epithelial Cell ◽  
Cell Polarity ◽  
Automated Analysis ◽  
Epithelial Cell Polarity

The Par3/Par6/aPKC complex and epithelial cell polarity

2013 ◽  
Vol 319 (10) ◽  
pp. 1357-1364 ◽  
Author(s):  
Jia Chen ◽  
Mingjie Zhang
Keyword(s):  
Epithelial Cell ◽  
Cell Polarity ◽  
Epithelial Cell Polarity
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