In vitro and in vivo evaluation of improved EGFR targeting peptide-conjugated phthalocyanine photosensitizers for tumor photodynamic therapy

2018 ◽  
Vol 29 (7) ◽  
pp. 1171-1178 ◽  
Author(s):  
Qingle Chen ◽  
Yanhong Ma ◽  
Jisi Zhao ◽  
Mei Zhao ◽  
Wenjing Li ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
In Vivo Evaluation ◽  
Targeting Peptide

Discovery of a Monoiodo Aza-BODIPY Near-Infrared Photosensitizer: in vitro and in vivo Evaluation for Photodynamic Therapy

2020 ◽  
Vol 63 (17) ◽  
pp. 9950-9964 ◽  
Author(s):  
Zhiliang Yu ◽  
Junliang Zhou ◽  
Xin Ji ◽  
Guangyu Lin ◽  
Shuang Xu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Near Infrared ◽  
In Vivo Evaluation

scholarly journals Biofilm Inactivation using Photodynamic Therapy in Dentistry: a review of literature

2020 ◽  
Vol 11 (Vol.11, no.3) ◽  
pp. 279-287
Author(s):  
Corina-Elena TIȘLER ◽  
Mîndra-Eugenia BADEA ◽  
Smaranda BUDURU ◽  
Andreea KUI ◽  
Mihaela FLORIA ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Chemical Substance ◽  
Review Of Literature ◽  
In Vivo Evaluation ◽  
Specific Effects ◽  
Pubmed Database ◽  
Microorganism Inactivation ◽  
Additional Procedure

Introduction: Photodynamic therapy (PDT) is a therapy involving light and a photosensitising chemical substance, used in conjunction with molecular oxygen in order to elicit cell death (photo-toxicity) and thus ability to kill microbial cells, including bacteria, fungi and viruses. Photodynamic therapy is an alternative method of biofilm disruption and it is considered a new way of microorganism inactivation. It is also an additional procedure to reduce the infection rate in patients, caused by the increasing antimicrobials resistance of bacteria. The aim of this literature review was to evaluate the specific effects and the antibacterial effectiveness of photodynamic therapy using different types of photosensitizers (Erythrosine, Rose Bengal, Toluidine blue, Methylene blue, Ozone, Riboflavin, Curcumin, Chlorhexidine, SAPYR) and a visible light of a specific wavelength for each photosensitizer and to reveal the applications of PDT in periodontics, endodontics, prosthodontics and dental caries. Methods: A research of literature was performed in an attempt to find all the articles published on this topic in the last 10 years. The articles was searched by using a certain combination of different keywords (photodynamic therapy ) and (diode laser ) and (teeth) in PubMed database. Results: A total number of 83 articles were found. After applying inclusion and exclusion criteria, 35 articles were taken into consideration for our study and among them 4 were a manuscript, 3 was a review of literature, 1 was an in vivo evaluation and 27 were in vitro studies. Conclusion: Considering that none of the disinfection methods can completely remove the biofilm, PDT is a therapeutic tool complementary to conventional disinfection, with great applicability in dentistry. PDT showed significantly efficacy in reduction of biofilms. Exposure to light in the presence of a photosensitizing chemical substance helps in the reduction of microbes and the protocols could be recommended for clinical usage, but only together with ‘classic ‘ disinfection.

In vitro and in vivo evaluation of Radachlorin® sensitizer for photodynamic therapy

2009 ◽  
Vol 8 (3) ◽  
pp. 405 ◽  
Author(s):  
Samuel Douillard ◽  
David Olivier ◽  
Thierry Patrice
Keyword(s):  
Photodynamic Therapy ◽  
In Vivo Evaluation

scholarly journals Self-assembled nanoparticles containing photosensitizer and polycationic brush for synergistic photothermal and photodynamic therapy against periodontitis

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Enyu Shi ◽  
Liya Bai ◽  
Lujia Mao ◽  
Hanping Wang ◽  
Xiaoying Yang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Chronic Inflammatory Disease ◽  
Bacterial Cells ◽  
Sprague Dawley ◽  
In Vivo Evaluation ◽  
Self Assembled ◽  
Bacterial Cell Membrane ◽  
Pass Through

Abstract Background Periodontitis is a chronic inflammatory disease in oral cavity owing to bacterial infection. Photothermal therapy (PTT) and photodynamic therapy (PDT) have many advantages for antibacterial treatment. As an excellent photosensitizer, indocyanine green (ICG) shows prominent photothermal and photodynamic performances. However, it is difficult to pass through the negatively charged bacterial cell membrane, thus limiting its antibacterial application for periodontitis treatment. Results In this work, self-assembled nanoparticles containing ICG and polycationic brush were prepared for synergistic PTT and PDT against periodontitis. First, a star-shaped polycationic brush poly(2-(dimethylamino)ethyl methacrylate) (sPDMA) was synthesized via atom transfer radical polymerization (ATRP) of DMA monomer from bromo-substituted β-cyclodextrin initiator (CD-Br). Next, ICG was assembled with sPDMA to prepare ICG-loaded sPDMA (sPDMA@ICG) nanoparticles (NPs) and the physicochemical properties of these NPs were characterized systematically. In vitro antibacterial effects of sPDMA@ICG NPs were investigated in porphyromonas gingivalis (Pg), one of the recognized periodontitis pathogens. A ligature-induced periodontitis model was established in Sprague–Dawley rats for in vivo evaluation of anti-periodontitis effects of sPDMA@ICG NPs. Benefiting from the unique brush-shaped architecture of sPDMA polycation, sPDMA@ICG NPs significantly promoted the adsorption and penetration of ICG into the bacterial cells and showed excellent PTT and PDT performances. Both in vitro and in vivo, sPDMA@ICG NPs exerted antibacterial and anti-periodontitis actions via synergistic PTT and PDT. Conclusions A self-assembled nanosystem containing ICG and polycationic brush has shown promising clinical application for synergistic PTT and PDT against periodontitis. Graphical Abstract

Synthesis, and in vitro and in vivo evaluation of a diphenylchlorin sensitizer for photodynamic therapy

2003 ◽  
Vol 69 (3) ◽  
pp. 179-192 ◽  
Author(s):  
L Bourré ◽  
G Simonneaux ◽  
Y Ferrand ◽  
S Thibaut ◽  
Y Lajat ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
In Vivo Evaluation

scholarly journals Initial In Vitro and In Vivo Evaluation of a Novel CCK2R Targeting Peptide Analog Labeled with Lutetium-177

Molecules ◽  
2020 ◽  
Vol 25 (19) ◽  
pp. 4585
Author(s):  
Anton Amadeus Hörmann ◽  
Maximilian Klingler ◽  
Maliheh Rezaeianpour ◽  
Nikolas Hörmann ◽  
Ronald Gust ◽  
...  
Keyword(s):  
Amino Acid ◽  
Enzymatic Degradation ◽  
Cell Uptake ◽  
Receptor Interaction ◽  
In Vivo Evaluation ◽  
Linear Peptide ◽  
Peptide Analog ◽  
Targeting Peptide

Targeting of cholecystokinin-2 receptor (CCK2R) expressing tumors using radiolabeled minigastrin (MG) analogs is hampered by rapid digestion of the linear peptide in vivo. In this study, a new MG analog stabilized against enzymatic degradation was investigated in preclinical studies to characterize the metabolites formed in vivo. The new MG analog DOTA-DGlu-Pro-Tyr-Gly-Trp-(N-Me)Nle-Asp-1Nal-NH2 comprising site-specific amino acid substitutions in position 2, 6 and 8 and different possible metabolites thereof were synthesized. The receptor interaction of the peptide and selected metabolites was evaluated in a CCK2R-expressing cell line. The enzymatic stability of the 177Lu-labeled peptide analog was evaluated in vitro in different media as well as in BALB/c mice up to 1 h after injection and the metabolites were identified based on radio-HPLC analysis. The new radiopeptide showed a highly increased stability in vivo with >56% intact radiopeptide in the blood of BALB/c mice 1 h after injection. High CCK2R affinity and cell uptake was confirmed only for the intact peptide, whereas enzymatic cleavage within the receptor specific C-terminal amino acid sequence resulted in complete loss of affinity and cell uptake. A favorable biodistribution profile was observed in BALB/c mice with low background activity, preferential renal excretion and prolonged uptake in CCK2R-expressing tissues. The novel stabilized MG analog shows high potential for diagnostic and therapeutic use. The radiometabolites characterized give new insights into the enzymatic degradation in vivo.

In Vitro and In Vivo Evaluation of Hypericin for Photodynamic Therapy of Equine Sarcoids

2000 ◽  
Vol 159 (1) ◽  
pp. 77-84 ◽  
Author(s):  
A. MARTENS ◽  
A.D.E. MOOR ◽  
E. WAELKENS ◽  
W. MERLEVEDE ◽  
P. DE WITTE
Keyword(s):  
Photodynamic Therapy ◽  
In Vivo Evaluation

In vitro and in vivo evaluation of the anti-inflammatory effects of Arzanol from Helichrysum italicum

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
J Bauer ◽  
F Dehm ◽  
A Koeberle ◽  
F Pollastro ◽  
G Appendino ◽  
...  
Keyword(s):  
In Vivo Evaluation ◽  
Anti Inflammatory
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