Challenges in biomarker discovery with MALDI-TOF MS

2016 ◽  
Vol 458 ◽  
pp. 84-98 ◽  
Author(s):  
Joanna Hajduk ◽  
Jan Matysiak ◽  
Zenon J. Kokot
Keyword(s):  
Biomarker Discovery ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Nanostructured Affinity Surfaces for MALDI-TOF-MS–Based Protein Profiling and Biomarker Discovery

2009 ◽  
pp. 421-456
Author(s):  
R. M. Vallant ◽  
M. Rainer ◽  
M. Najam-Ul-Haq ◽  
R. Bakry ◽  
C. Petter ◽  
...  
Keyword(s):  
Biomarker Discovery ◽  
Protein Profiling ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Rapid Discrimination and Authentication of Korean Farmstead Mozzarella Cheese through MALDI-TOF and Multivariate Statistical Analysis

Metabolites ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 333
Author(s):  
Sujatha Kandasamy ◽  
Jayeon Yoo ◽  
Jeonghee Yun ◽  
Han-Byul Kang ◽  
Kuk-Hwan Seol ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Multivariate Statistical Analysis ◽  
Biomarker Discovery ◽  
Geographical Location ◽  
Accurate Method ◽  
Multivariate Statistical ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Geographical origin and authenticity are the two crucial factors that propel overall cheese perception in terms of quality and price; therefore, they are of great importance to consumers and commercial cheese producers. Herein, we demonstrate a rapid, accurate method for discrimination of domestic and import mozzarella cheeses in the Republic of Korea by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The protein profiles’ data aided by multivariate statistical analysis successfully differentiated farmstead and import mozzarella cheeses according to their geographical location of origin. A similar investigation within domestic samples (farmsteads/companies) also showed clear discrimination regarding the producer. Using the biomarker discovery tool, we identified seven distinct proteins, of which two (m/z 7407.8 and 11,416.6) were specific in farmstead cheeses, acting as potential markers to ensure authentication and traceability. The outcome of this study can be a good resource in building a database for Korean domestic cheeses. This study also emphasizes the combined utility of MALDI-TOF MS and multivariate analysis in preventing fraudulent practices, thereby ensuring market protection for Korean farmstead cheeses.

scholarly journals Optimized Preparation of Urine Samples from Acute Melioidosis Patients for In-Solution Proteomic Studies using LCMS QTOF or MALDI TOF MS

2021 ◽  
Vol 20 (3) ◽  
Author(s):  
Aniza Pakeer ◽  
Mohammed Imad A. Mustafa Mahmud ◽  
Nosrihah Ismail ◽  
Vanitha Mariappan
Keyword(s):  
Biomarker Discovery ◽  
Acute Phase Proteins ◽  
Protein Precipitation ◽  
Urine Samples ◽  
Precipitation Method ◽  
Cell Mediated Immunity ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Culture Positive ◽  
Tof Ms

Introduction: Investigation of urine proteome in patients with acute melioidosis may reveal potential disease markers, from either bacterial or human proteins. We used an in-solution gel-free method instead of 2-DE to detect human and Burkholderia pseudomallei proteins in urine of patients with acute melioidosis. Here, we propose a simpler, economical method for preparing urine samples directly from melioidosis patients, for in-solution proteomic analysis using LCMS-QTOF MS/MS or MALDI-TOF MS/MS. Material and Methods: We adapted an acetone-TCA based protein precipitation method with LCMS-QTOF MS to detect the B. pseudomallei proteins directly from urine of acute melioidosis patients (culture positive and negative). This process involves protein precipitation, desalting, trypsin digestion, and optimization for the mass spectrometry. Results: A total of 3,866 human peptides were detected across 11 urine samples from clinically suspected acute melioidosis patients. Among them were three Burkholderia specific proteins detected in 75% of culture positive samples. Large amounts of acute phase proteins, cell mediated immunity proteins, complement pathway proteins and inflammatory mediators were seen upon gene ontology (GO) annotation and GO enrichment analysis. Conclusions: This simple in-solution sample preparation method can be replicated easily for LCMS/MS-QTOF and MALDI-TOF proteomic analyses, avoiding tedious optimization steps in 2-DE. This method is cost effective and can be done in centres without specialized 2-DE or MS equipment and elutes can be easily transported for analysis and bioinformatics. This is the first study to analyse urine samples directly for B. pseudomallei proteins. Discovery of the entire proteome as a whole is important in leading to biomarker discovery.

scholarly journals Biomarker Discovery and Redundancy Reduction towards Classification using a Multi-factorial MALDI-TOF MS T2DM Mouse Model Dataset

2011 ◽  
Vol 12 (1) ◽  
Author(s):  
Chris Bauer ◽  
Frank Kleinjung ◽  
Celia J Smith ◽  
Mark W Towers ◽  
Ali Tiss ◽  
...  
Keyword(s):  
Mouse Model ◽  
Biomarker Discovery ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Redundancy Reduction ◽  
Tof Ms

Peptidome profiling of induced sputum by mesoporous silica beads and MALDI-TOF MS for non-invasive biomarker discovery of chronic inflammatory lung diseases

PROTEOMICS ◽  
2011 ◽  
Vol 11 (16) ◽  
pp. 3402-3414 ◽  
Author(s):  
Rosa Terracciano ◽  
Mariaimmacolata Preianò ◽  
Grazia P. Palladino ◽  
Giovanna E. Carpagnano ◽  
Maria P. Foschino Barbaro ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Lung Diseases ◽  
Biomarker Discovery ◽  
Induced Sputum ◽  
Maldi Tof Ms ◽  
Non Invasive ◽  
Maldi Tof ◽  
Silica Beads ◽  
Tof Ms

895: Proteomic Analysis of Sera from Bladder Cancer Patients with MALDI-TOF-MS

2007 ◽  
Vol 177 (4S) ◽  
pp. 297-297
Author(s):  
Kristina Schwamborn ◽  
Rene Krieg ◽  
Ruth Knüchel-Clarke ◽  
Joachim Grosse ◽  
Gerhard Jakse
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Proteomic Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

HPTLC-MALDI/TOF/MS vs. HPLC-ESI/MS2 for identification to flavonoids in plant extracts

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
L Fougère ◽  
D Da Silva ◽  
E Destandau ◽  
C Elfakir
Keyword(s):  
Plant Extracts ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Infektionserreger beim Schwein – Analyse des kultivierbaren Mikrobioms mittels MALDI-TOF MS

2017 ◽  
Author(s):  
M Erhard ◽  
M Metzner ◽  
D Köhler-Repp ◽  
B Köhler ◽  
R Storandt
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Quantitative metabolomics of Iranian saffron based on their HPLC-DAD and MALDI-TOF-MS

2019 ◽  
Author(s):  
M Hooshyari ◽  
H Rezadoost ◽  
P Ghezellou ◽  
A Ghassempour
Keyword(s):  
Maldi Tof Ms ◽  
Quantitative Metabolomics ◽  
Maldi Tof ◽  
Tof Ms
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