Locked nucleic acid probe enhances Sanger sequencing sensitivity and improves diagnostic accuracy of high-resolution melting-based KRAS mutational analysis

2016 ◽  
Vol 457 ◽  
pp. 75-80 ◽  
Author(s):  
Takayuki Ishige ◽  
Sakae Itoga ◽  
Kazuyuki Matsushita ◽  
Fumio Nomura
Keyword(s):  
Nucleic Acid ◽  
High Resolution ◽  
Diagnostic Accuracy ◽  
Sanger Sequencing ◽  
High Resolution Melting ◽  
Mutational Analysis ◽  
Locked Nucleic Acid ◽  
Locked Nucleic Acid Probe

scholarly journals Locked Nucleic Acid Probe-Based Real-Time PCR Assay for the Rapid Detection of Rifampin-Resistant Mycobacterium tuberculosis

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0143444 ◽  
Author(s):  
Yong Zhao ◽  
Guilian Li ◽  
Chongyun Sun ◽  
Chao Li ◽  
Xiaochen Wang ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Locked Nucleic Acid ◽  
Pcr Assay ◽  
Locked Nucleic Acid Probe

Differentiation of Scomber japonicus from Scomber scombrus by using a single locked nucleic acid probe

2015 ◽  
Vol 28 (3) ◽  
pp. 379-382 ◽  
Author(s):  
Jeong Jin Ahn ◽  
Youngjoo Kim ◽  
Ji Young Hong ◽  
Gi Won Kim ◽  
Seol Young Kim ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Locked Nucleic Acid ◽  
Scomber Japonicus ◽  
Scomber Scombrus ◽  
Locked Nucleic Acid Probe

scholarly journals Comparative evaluation of the new FDA approved THxID™-BRAF test with high resolution melting and sanger sequencing

BMC Cancer ◽  
2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Julie Marchant ◽  
Alain Mange ◽  
Marion Larrieux ◽  
Valérie Costes ◽  
Jérôme Solassol
Keyword(s):  
High Resolution ◽  
Comparative Evaluation ◽  
Sanger Sequencing ◽  
High Resolution Melting

Multiplex genotyping based on the melting temperature of a single locked nucleic acid probe

2015 ◽  
Vol 491 ◽  
pp. 72-74 ◽  
Author(s):  
Jeong Jin Ahn ◽  
Ji-Hoon Kim ◽  
Youngjoo Kim ◽  
Ji Young Hong ◽  
Gi Won Kim ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Melting Temperature ◽  
Locked Nucleic Acid ◽  
Multiplex Genotyping ◽  
Locked Nucleic Acid Probe

Locked nucleic acid probe-based real-time PCR for the diagnosis of Listeria monocytogenes in ruminants

2016 ◽  
Vol 30 (3) ◽  
pp. 138-145 ◽  
Author(s):  
Mohamed Barkallah ◽  
Yaakoub Gharbi ◽  
Mariam Hmani ◽  
Zouhir Mallek ◽  
Michel Gautier ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Locked Nucleic Acid ◽  
Locked Nucleic Acid Probe

Analysis of the genetic stability of event NK603 in stacked corn varieties using high-resolution melting (HRM) analysis and Sanger sequencing

2016 ◽  
Vol 243 (3) ◽  
pp. 353-365 ◽  
Author(s):  
Magali Castan ◽  
Sina-Elisabeth Ben Ali ◽  
Rupert Hochegger ◽  
Werner Ruppitsch ◽  
Alexander G. Haslberger ◽  
...  
Keyword(s):  
High Resolution ◽  
Genetic Stability ◽  
Sanger Sequencing ◽  
High Resolution Melting ◽  
Hrm Analysis

scholarly journals Use of the HRM Method in Quick Identification of FecXO Mutation in Highly Prolific Olkuska Sheep

Animals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 844
Author(s):  
Grzegorz Smołucha ◽  
Katarzyna Piórkowska ◽  
Katarzyna Ropka-Molik ◽  
Jacek Sikora
Keyword(s):  
High Resolution ◽  
Molecular Biology ◽  
Sanger Sequencing ◽  
Genetic Basis ◽  
High Resolution Melting ◽  
Sheep Breed ◽  
Causative Mutation ◽  
Sequencing Method

Olkuska is a highly prolific sheep breed in Poland. Thanks to earlier identification of the genetic basis of its prolificacy, a mutation in the BMP-15 gene, we can use molecular biology tools to identify this causative mutation affecting prolificacy. In our research, we used the High-Resolution Melting (HRM) and Sanger sequencing methods to identify the genotypes of the studied animals. The result obtained by the HRM method is identical to those obtained by the sequencing method, which confirms the effectiveness of the HRM method and the possibility of quick and cheap identification of individuals with a FecXO mutation.

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