Rapid detection of PML–RARA fusion gene by novel high-speed droplet-reverse transcriptase-polymerase chain reaction: Possibility for molecular diagnosis without lagging behind the morphological analyses

2013 ◽  
Vol 415 ◽  
pp. 276-278 ◽  
Author(s):  
Akane Sueki ◽  
Kazuyuki Matsuda ◽  
Chiaki Taira ◽  
Akemi Yamaguchi ◽  
Hiroshi Koeda ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Molecular Diagnosis ◽  
Rapid Detection ◽  
High Speed ◽  
Fusion Gene ◽  
Chain Reaction ◽  
Polymerase Chain

Reverse Transcriptase Polymerase Chain Reaction on Fine Needle Aspirates for Rapid Detection of Translocations in Synovial Sarcoma

1998 ◽  
Vol 42 (6) ◽  
pp. 1317-1324 ◽  
Author(s):  
Gunnar Nilsson ◽  
Min Wang ◽  
Johan Wejde ◽  
Lena Kanter ◽  
Jonas Karlén ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Synovial Sarcoma ◽  
Rapid Detection ◽  
Chain Reaction ◽  
Fine Needle ◽  
Fine Needle Aspirates ◽  
Polymerase Chain

Frequency of two transcripts of BCR-ABL 1 fusion gene (p210 and p190) in acute lymphoblastic leukemia and chronic myeloid leukemia by reverse transcriptase polymerase chain reaction

2021 ◽  
Vol 9 (1) ◽  
pp. 12-15
Author(s):  
Maria Altaf ◽  
Saleem Ahmed Khan ◽  
Ayesha Khan ◽  
Ammara Arsalan
Keyword(s):  
Polymerase Chain Reaction ◽  
Acute Lymphoblastic Leukemia ◽  
Reverse Transcriptase ◽  
Real Time ◽  
Fusion Gene ◽  
Lymphoblastic Leukemia ◽  
Prognostic Significance ◽  
Myelogenous Leukemia ◽  
Chain Reaction ◽  
Polymerase Chain

Objective: To determine the frequency of two transcripts of BCR-ABL 1 fusion gene (P210 & P190) in acute lymphoblastic leukemia and chronic myelogenous leukemia by reverse transcriptase polymerase chain reaction. Study design: A cross-sectional study. Place and duration of study: The Armed Forces Institute of Pathology (AFIP), Rawalpindi, from December 2012 to January 2014. Methodology: 147 diagnosed patients of CML and ALL were subjected to real time reverse transcriptase polymerase chain reaction. For PCR,2-3ml of venous blood in EDTA was collected .RNA extraction was done by Trizol Reagent LS (MRC, USA) and cDNA was synthesized using reverse transcriptase and gene specific primer. Real time- PCR was done on ABI-7500.The positive samples were identified when fluorescence exceeded threshold limit. Results: Out of 147 samples, 85 were with diagnosis of CML. All of them (100%) showed P210. 58 patients had diagnosis of ALL. Out of these BCR-ABL1 is detected in 11(18.9%) patients. 9(81.8%) expressed P190 whereas P210 was present in 2(18.1%) patients. 4 patients in lymphoid blast phase of CML were identified. 3(75%) of them had PP210 and 1(25%) shows both (P190 & P210) the transcripts. Conclusion: All CML patients expressed P210 transcript whereas ALL patients mostly showed P190. These transcripts expressing specific tyrosine kinase protein have diagnostic as well prognostic significance.

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