A comparative proteomics analysis of peritoneal dialysate before and after the occurrence of peritonitis episode by mass spectrometry

2013 ◽  
Vol 420 ◽  
pp. 34-44 ◽  
Author(s):  
Yu-Chang Tyan ◽  
Shih-Bin Su ◽  
Sing-Sing Ting ◽  
Hsien-Yi Wang ◽  
Pao-Chi Liao
Keyword(s):  
Mass Spectrometry ◽  
Comparative Proteomics ◽  
Proteomics Analysis ◽  
Peritoneal Dialysate ◽  
Before And After

Comparative proteomics analysis of silkworm hemolymph during the stages of metamorphosis via liquid chromatography and mass spectrometry

PROTEOMICS ◽  
2016 ◽  
Vol 16 (9) ◽  
pp. 1421-1431 ◽  
Author(s):  
Yong Hou ◽  
Yan Zhang ◽  
Jing Gong ◽  
Sha Tian ◽  
Jianwei Li ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Comparative Proteomics ◽  
Proteomics Analysis ◽  
Silkworm Hemolymph

scholarly journals Redox Mechanism of Azathioprine and Its Interaction with DNA

2021 ◽  
Vol 22 (13) ◽  
pp. 6805
Author(s):  
Mihaela-Cristina Bunea ◽  
Victor-Constantin Diculescu ◽  
Monica Enculescu ◽  
Horia Iovu ◽  
Teodor Adrian Enache
Keyword(s):  
Electron Microscopy ◽  
Mass Spectrometry ◽  
Scanning Electron Microscopy ◽  
Structural Changes ◽  
Differential Pulse ◽  
Immunosuppressive Drug ◽  
Redox Mechanism ◽  
Before And After ◽  
Dna Film ◽  
Scanning Electron

The electrochemical behavior and the interaction of the immunosuppressive drug azathioprine (AZA) with deoxyribonucleic acid (DNA) were investigated using voltammetric techniques, mass spectrometry (MS), and scanning electron microscopy (SEM). The redox mechanism of AZA on glassy carbon (GC) was investigated using cyclic and differential pulse (DP) voltammetry. It was proven that the electroactive center of AZA is the nitro group and its reduction mechanism is a diffusion-controlled process, which occurs in consecutive steps with formation of electroactive products and involves the transfer of electrons and protons. A redox mechanism was proposed and the interaction of AZA with DNA was also investigated. Morphological characterization of the DNA film on the electrode surface before and after interaction with AZA was performed using scanning electron microscopy. An electrochemical DNA biosensor was employed to study the interactions between AZA and DNA with different concentrations, incubation times, and applied potential values. It was shown that the reduction of AZA molecules bound to the DNA layer induces structural changes of the DNA double strands and oxidative damage, which were recognized through the occurrence of the 8-oxo-deoxyguanosine oxidation peak. Mass spectrometry investigation of the DNA film before and after interaction with AZA also demonstrated the formation of AZA adducts with purine bases.

scholarly journals Light-Induced Byproducts of Vitamin C in Multivitamin Solutions

2004 ◽  
Vol 50 (1) ◽  
pp. 135-140 ◽  
Author(s):  
Jean-Claude Lavoie ◽  
Philippe Chessex ◽  
Thérèse Rouleau ◽  
Diane Migneault ◽  
Blandine Comte
Keyword(s):  
Mass Spectrometry ◽  
Ascorbic Acid ◽  
Vitamin C ◽  
Organic Peroxides ◽  
Ambient Light ◽  
Rapid Loss ◽  
Biological Importance ◽  
Before And After

Abstract Background: When solutions of multivitamin preparations (MVPs) are exposed to light, H2O2 as well as organic peroxides are generated and the concentration of vitamin C decreases. The aim of this study was to determine, using mass spectrometry, whether the generation of oxidative byproducts of vitamin C, such as dehydroascorbate (DHA) and 2,3-diketogulonic acid (DKG), accounted for the reported decrease in ascorbic acid in MVPs exposed to light. Methods: Mass spectrometry was used to document the formation of byproducts of ascorbic acid in solutions containing a MVP, vitamin C + riboflavin, and vitamin C + H2O2 + Fe2+. The involvement of ascorbic acid and H2O2 in the formation of organic peroxides was tested by measuring peroxide concentrations in solutions containing H2O2 with or without ascorbic acid and with or without Fe2+ before and after addition of catalase. Results: The loss of ascorbic acid in photo-exposed MVPs was associated with the concomitant generation of byproducts different from DHA and DKG. Among them, one mass fingerprint was particularly observed with solutions of vitamin C + riboflavin exposed to ambient light as well as with the solution of vitamin C + H2O2 + Fe2+, suggesting a Fenton-like reaction. This fingerprint was associated with the formation of catalase-resistant peroxides. Conclusion: Exposure of MVPs to light leads to the rapid loss of ascorbic acid and generation of specific byproducts that differ from DHA and DKG. The conversion of vitamin C into byproducts could be of biological importance in accounting for the decrease in ascorbic acid concentrations and the generation of organic peroxides in light-exposed MVPs.

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