Correlation between enzyme immunoassay and indirect immunofluorescence test for high titer of anti-ribosomal-P antibodies

2012 ◽  
Vol 413 (5-6) ◽  
pp. 650-652 ◽  
Author(s):  
Kyeong-Hee Kim ◽  
Bo-Ram Kim ◽  
Ji-Eun Kim ◽  
Kwang-Sook Woo ◽  
Jin-Yeong Han ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Indirect Immunofluorescence ◽  
High Titer ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Ribosomal P

scholarly journals Simple diagnostic test for antibodies to Encephalitozoon cuniculi based on enzyme immunoassay

1981 ◽  
Vol 15 (1) ◽  
pp. 41-44 ◽  
Author(s):  
J. C. Cox ◽  
R. Horsburgh ◽  
D. Pye
Keyword(s):  
Cell Culture ◽  
Enzyme Immunoassay ◽  
Diagnostic Test ◽  
Indirect Immunofluorescence ◽  
Antigen Processing ◽  
Encephalitozoon Cuniculi ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Specialized Equipment

Rabbit antibodies against Encephalitozoon cuniculi were detected in an enzyme immunoassay procedure in which antigen was grown and used in situ. The test appeared to be more sensitive than the indirect immunofluorescence test with which it was compared, but gave essentially the same results for the 64 sera evaluated. This procedure will allow any laboratory with cell-culture facilities to produce a diagnostic antigen without the need for antigen processing. It is simple and reliable, and does not require specialized equipment or microscopic assessment.

Recognition of a Non-HLA-ABC Antigen Present on B and T Lymphocytes and Monocytes only Detectable with the Indirect Immunofluorescence Test

Vox Sanguinis ◽  
1979 ◽  
Vol 36 (3) ◽  
pp. 150-158
Author(s):  
F. Décary ◽  
F.W.A. Verheugt ◽  
L. van Helden-Henningheim ◽  
E. von Riesz ◽  
R. Schreuder-van Gelder ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Indirect Immunofluorescence ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Antigen Present ◽  
B And T Lymphocytes

scholarly journals Behaviour of indirect immunofluorescence test and some cerebrospinal parameters in neurocysticercosis

1987 ◽  
Vol 45 (1) ◽  
pp. 33-43 ◽  
Author(s):  
Amauri Braga Simonetti ◽  
Jorge Teixeira
Keyword(s):  
Cerebrospinal Fluid ◽  
Indirect Immunofluorescence ◽  
False Positive ◽  
Clinical Evidence ◽  
Routine Analysis ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Immunofluorescent Test ◽  
Positive Results

Cerebrospinal fluid from 53 patients with clinical evidence of neurocysticercosis and 11 who suffered from several diseases were studied to evaluate the behaviour of indirect immunofluorescence test and some parameters of routine analysis. In neurocysticercosis there were pleocitosis in 88.7% of cases, eosinophilorrachia in 60.3%o, hyperproteinorrachia in 71.7% and hypoglucorrachia in 13.2%. The indirect immunofluorescence test was positive in 19.2% of cases but false-positive results were found when the samples showed xanthochromia or erythrocyte contamination. The authors discuss their results in comparison with those in literature and conclude that the immunofluorescent test is sensitive and useful in diagnosis of neurocysticercosis, except when the interferents previously mentioned are present.

Evaluation of the automated indirect immunofluorescence test for anti-dsDNA antibodies

2019 ◽  
Vol 498 ◽  
pp. 143-147 ◽  
Author(s):  
Shiji Wu ◽  
Feng Wang ◽  
Jin Huang ◽  
Botao Yin ◽  
Min Huang ◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Dsdna Antibodies

Therapy of Alveococcosis in Man

1977 ◽  
Vol 5 (5) ◽  
pp. 367-368 ◽  
Author(s):  
C E Lüdin ◽  
K Gyr ◽  
K Karoussos
Keyword(s):  
Partial Hepatectomy ◽  
Indirect Immunofluorescence ◽  
The Other ◽  
Immunofluorescence Test ◽  
Serological Tests ◽  
Passive Haemagglutination ◽  
Indirect Immunofluorescence Test ◽  
Reliable Methods

Diagnostic, therapeutic and prognostic aspects are interpreted on the basis of two cases of Echinococcus alveolaris. Both patients had undergone partial hepatectomy and were subsequently treated chemotherapeutically. In one case dehydroemetine and mebendazole (Vermox®, Janssen), and in the other case metrifonate (Bilarcil®, Bayer A. G.) were used. The diagnosis of alveococcosis of the liver was demonstrated in one case histologically on the occasion of appendectomy, and in the other case by the indirect immunofluorescence test and passive haemagglutination. In case of suspected alveococcosis these serological tests are imperative, as they are the most reliable methods of demonstrating this disease. The latest trend in therapy aims at a combination of surgery and chemotherapy. Mebendazole seems to be promising as an anthelmintic agent for the treatment of alveococcosis. Remission of the disease was obtained in either case.

Isolation of the Causative Organism of Canine Encephalitozoonosis

1978 ◽  
Vol 15 (4) ◽  
pp. 449-460 ◽  
Author(s):  
J. A. Shadduck ◽  
R. Bendele ◽  
G. T. Robinson
Keyword(s):  
Endothelial Cells ◽  
Indirect Immunofluorescence ◽  
Renal Tubule ◽  
Causative Organism ◽  
Encephalitozoon Cuniculi ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Tubule Cells ◽  
Spontaneous Infection

Spontaneous infection with Encephalitozoon cuniculi resulted in a lethal disease in all but one of a litter of puppies in Texas. The disease was characterized by severe nonsuppurative nephritis, encephalitis and segmental vasculitis. Many protozoa were in renal tubule cells, endothelial cells and brain. The number of organisms decreased and the granulomatous character of the lesions became more prominent as the disease progressed. Sera from affected puppies and their parents reacted in an indirect immunofluorescence test with canine E. cuniculi propagated in vitro. The sera of owners of affected pups were negative.

scholarly journals Q fever in the Netherlands: a sero-epidemiological survey among human population groups from 1968 to 1983

1987 ◽  
Vol 98 (2) ◽  
pp. 211-219 ◽  
Author(s):  
J. H. Richardus ◽  
A. Donkers ◽  
A. M. Dumas ◽  
G. J. P. Schaap ◽  
J. P. W. M. Akkermans ◽  
...  
Keyword(s):  
The Netherlands ◽  
Indirect Immunofluorescence ◽  
Q Fever ◽  
Epidemiological Survey ◽  
Immunofluorescence Test ◽  
Age Classes ◽  
Serum Samples ◽  
Occupational Groups ◽  
Indirect Immunofluorescence Test ◽  
Long Time

SUMMARYA sero-epidemiological survey, using an indirect immunofluorescence test for IgG against Coxiella burnetii (phase II), was carried out in the Netherlands. Serum samples taken in 1968, 1975, 1979 and 1983 were tested. Occupational groups with a supposedly high risk of infection (veterinarians, residents of dairy farms and taxidermists) showed a significantly higher percentage of seropositives than defined controls. The percentage of seropositive amateur wool spinners was significantly higher than that of the controls from the same region. Since 1968 there has been no increase in the percentage of infected persons, indicating that, contrary to earlier assumptions, Q fever has been endemic in The Netherlands for a long time already. The increase in numbers of notified cases of overt Q fever is considered to be the result of the recent introduction of a sensitive indirect immunofluorescence test for IgM antibodies against C. burnetii. Antibody percentages in all age classes between 1 and 64 years were much alike, suggesting that most infections occur in early childhood. This is in accordance with the finding that 35% of our patients are younger than 3 years. The possibility of infection related to childbirth and lactation is discussed.

Sign in / Sign up

Export Citation Format

Share Document