A simple and economic quadruplex one-step reverse transcription-PCR assay for detecting and typing dengue viruses

2011 ◽  
Vol 412 (21-22) ◽  
pp. 2008-2010
Author(s):  
C.L.H. Lo ◽  
S.P. Yip ◽  
S.J. Wei ◽  
J.E. Levi ◽  
P.H.M. Leung
Keyword(s):  
Reverse Transcription ◽  
Pcr Assay ◽  
Dengue Viruses ◽  
Reverse Transcription Pcr ◽  
One Step

Rapid diagnosis of enterovirus infection by a new one-step reverse transcription-PCR assay.

1997 ◽  
Vol 35 (4) ◽  
pp. 976-977 ◽  
Author(s):  
H H Kessler ◽  
B Santner ◽  
H Rabenau ◽  
A Berger ◽  
A Vince ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Rapid Diagnosis ◽  
Enterovirus Infection ◽  
Pcr Assay ◽  
Reverse Transcription Pcr ◽  
One Step

scholarly journals An Improved One-Step Real-Time Reverse Transcription-PCR Assay for Detection of Norovirus

2015 ◽  
Vol 54 (2) ◽  
pp. 497-499 ◽  
Author(s):  
Ilona Glowacka ◽  
Gabrielle Harste ◽  
Jennifer Witthuhn ◽  
Albert Heim
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Pcr Assay ◽  
Reverse Transcription Pcr ◽  
One Step

scholarly journals Rapid Simultaneous Detection of Enterovirus and Parechovirus RNAs in Clinical Samples by One-Step Real-Time Reverse Transcription-PCR Assay

2011 ◽  
Vol 49 (7) ◽  
pp. 2620-2624 ◽  
Author(s):  
Susan Bennett ◽  
Heli Harvala ◽  
Jeroen Witteveldt ◽  
E. Carol McWilliam Leitch ◽  
Nigel McLeish ◽  
...  
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Simultaneous Detection ◽  
Clinical Samples ◽  
Pcr Assay ◽  
Reverse Transcription Pcr ◽  
One Step

scholarly journals Implementation of an in-house real-time reverse transcription-PCR assay for the rapid detection of the SARS-CoV-2 Marseille-4 variant

2021 ◽  
Author(s):  
Marielle Bedotto ◽  
Pierre-Edouard Fournier ◽  
Linda Houhamdi ◽  
Anthony Levasseur ◽  
Jeremy Delerce ◽  
...  
Keyword(s):  
Immune Responses ◽  
Real Time ◽  
Reverse Transcription ◽  
Geographical Area ◽  
Viral Escape ◽  
Pcr Assay ◽  
Reverse Transcription Pcr ◽  
One Step ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing

ABSTRACTIntroductionThe SARS-CoV-2 pandemic has been associated with the occurrence since summer 2020 of several viral variants that overlapped or succeeded each other in time. Those of current concern harbor mutations within the spike receptor binding domain (RBD) that may be associated with viral escape to immune responses. In our geographical area a viral variant we named Marseille-4 harbors a S477N substitution in this RBD.Materials and methodsWe aimed to implement an in-house one-step real-time reverse transcription-PCR (qPCR) assay with a hydrolysis probe that specifically detects the SARS-CoV-2 Marseille-4 variant.ResultsAll 6 cDNA samples from Marseille-4 variant strains identified in our institute by genome next-generation sequencing (NGS) tested positive using our Marseille-4 specific qPCR, whereas all 32 cDNA samples from other variants tested negative. In addition, 39/42 (93%) respiratory samples identified by NGS as containing a Marseille-4 variant strain and 0/26 samples identified as containing non-Marseille-4 variant strains were positive. Finally, 1,585/2,889 patients SARS-CoV-2-diagnosed in our institute, 10/277 (3.6%) respiratory samples collected in Algeria, and none of 207 respiratory samples collected in Senegal, Morocco, or Lebanon tested positive using our Marseille-4 specific qPCR.DiscussionOur in-house qPCR system was found reliable to detect specifically the Marseille-4 variant and allowed estimating it is involved in more than half of our SARS-CoV-2 diagnoses since December 2020. Such approach allows the real-time surveillance of SARS-CoV-2 variants, which is warranted to monitor and assess their epidemiological and clinical characterics based on comprehensive sets of data.

Development and validation of real-time one-step reverse transcription-PCR for the detection and typing of dengue viruses

2009 ◽  
Vol 45 (1) ◽  
pp. 61-66 ◽  
Author(s):  
Isabelle Leparc-Goffart ◽  
Meili Baragatti ◽  
Sarah Temmam ◽  
Anne Tuiskunen ◽  
Gregory Moureau ◽  
...  
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Dengue Viruses ◽  
Reverse Transcription Pcr ◽  
One Step ◽  
Development And Validation
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