Apolipoprotein A-I, apolipoprotein B, and apolipoprotein B/apolipoprotein A-I ratio: Reference intervals compared with values in different pathophysiological conditions from the FINRISK 2007 study

2011 ◽  
Vol 412 (11-12) ◽  
pp. 1146-1150 ◽  
Author(s):  
Jaana Leiviskä ◽  
Jouko Sundvall ◽  
Georg Alfthan ◽  
Matti Jauhiainen ◽  
Veikko Salomaa
Keyword(s):  
Apolipoprotein B ◽  
Reference Intervals ◽  
Apolipoprotein A

Effects of lyophilization of serum on the measurement of apolipoproteins A-I and B

1990 ◽  
Vol 36 (2) ◽  
pp. 366-369 ◽  
Author(s):  
S M Marcovina ◽  
J L Adolphson ◽  
M Parlavecchia ◽  
J J Albers
Keyword(s):  
Reference Materials ◽  
Apolipoprotein B ◽  
Matrix Effects ◽  
Reference Intervals ◽  
Assay Method ◽  
Common Reference ◽  
Apolipoprotein A ◽  
Quality Control Materials ◽  
Standardization Process ◽  
The Difference

Abstract A common accuracy-based standardization program is indispensable for establishing reference intervals for the clinical use of apolipoproteins. The development and distribution of reference materials and quality-control materials that do not exhibit matrix effects between methods is essential to the standardization process. We examined the suitability of lyophilized material as a common reference material for the measurement of apolipoproteins A-I and B. We determined values for apolipoproteins A-I and B in frozen and lyophilized serum pools, using different immunochemical approaches. We found little or no differences in apolipoprotein A-I values between frozen and lyophilized pools as determined by the different methods. In contrast, values for apolipoprotein B in lyophilized samples were consistently lower than those obtained for frozen samples. After adjusting for the effect of dilution due to reconstitution, the difference in the apolipoprotein B values for lyophilized as compared with frozen samples ranged from -26% to 4%, depending upon the assay method. Evidently, serum pools in lyophilized from are not a suitable matrix for reference materials for apolipoprotein B measurements but can be used for apolipoprotein A-I measurements.

scholarly journals Familial hypertriglyceridemia: an entity with distinguishable features from other causes of hypertriglyceridemia

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Ivette Cruz-Bautista ◽  
Alicia Huerta-Chagoya ◽  
Hortensia Moreno-Macías ◽  
Rosario Rodríguez-Guillén ◽  
María Luisa Ordóñez-Sánchez ◽  
...  
Keyword(s):  
Apolipoprotein B ◽  
Rare Variants ◽  
Regulatory Proteins ◽  
Fibroblast Growth Factor 21 ◽  
Fibroblast Growth ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Apo B ◽  
Independent Components ◽  
Apolipoprotein A

Abstract Background Familial hypertriglyceridemia (FHTG) is a partially characterized primary dyslipidemia which is frequently confused with other forms hypertriglyceridemia. The aim of this work is to search for specific features that can help physicians recognize this disease. Methods This study included 84 FHTG cases, 728 subjects with common mild-to-moderate hypertriglyceridemia (CHTG) and 609 normotriglyceridemic controls. All subjects underwent genetic, clinical and biochemical assessments. A set of 53 single nucleotide polymorphisms (SNPs) previously associated with triglycerides levels, as well as 37 rare variants within the five main genes associated with hypertriglyceridemia (i.e. LPL, APOC2, APOA5, LMF1 and GPIHBP1) were analyzed. A panel of endocrine regulatory proteins associated with triglycerides homeostasis were compared between the FHTG and CHTG groups. Results Apolipoprotein B, fibroblast growth factor 21(FGF-21), angiopoietin-like proteins 3 (ANGPTL3) and apolipoprotein A-II concentrations, were independent components of a model to detect FHTG compared with CHTG (AUC 0.948, 95%CI 0.901–0.970, 98.5% sensitivity, 92.2% specificity, P < 0.001). The polygenic set of SNPs, accounted for 1.78% of the variance in triglyceride levels in FHTG and 6.73% in CHTG. Conclusions The clinical and genetic differences observed between FHTG and CHTG supports the notion that FHTG is a unique entity, distinguishable from other causes of hypertriglyceridemia by the higher concentrations of insulin, FGF-21, ANGPTL3, apo A-II and lower levels of apo B. We propose the inclusion of these parameters as useful markers for differentiating FHTG from other causes of hypertriglyceridemia.

scholarly journals Identification of the cysteine residue in apolipoprotein(a) that mediates extracellular coupling with apolipoprotein B-100.

1993 ◽  
Vol 268 (26) ◽  
pp. 19819-19825 ◽  
Author(s):  
M.L. Koschinsky ◽  
G.P. Côté ◽  
B Gabel ◽  
Y.Y. van der Hoek
Keyword(s):  
Apolipoprotein B ◽  
Cysteine Residue ◽  
Apolipoprotein A

Abstract 568: Lipidomic Comparison of Hyperglycemic and Normal Subjects Using Absolute Quantitation of >700 Lipid Species

2017 ◽  
Vol 37 (suppl_1) ◽  
Author(s):  
Jon Rees ◽  
Susan Kuklenyik ◽  
Michael Gardner ◽  
David Schieltz ◽  
Bryan A Parks ◽  
...  
Keyword(s):  
Apolipoprotein B ◽  
Particle Number ◽  
Normal Subjects ◽  
Absolute Quantitation ◽  
Insulin Resistant ◽  
Apolipoprotein A ◽  
Class Level ◽  
Lipid Species ◽  
Negative Health Outcomes ◽  
Normal Controls

The presence of diabetes mellitus in patients significantly increases the risk of cardiovascular disease, among many negative health outcomes. Several research groups have reported lipid species anomalies in subjects showing insulin resistance, including higher concentrations of sphingomyelin species relative to normal controls. Advances in lipidomic methodologies has allowed for the profiling of numerous lipid species in a single extraction and analytical run. We used the SCIEX Lipidyzer platform to determine the absolute concentration of 770 distinct lipid species from 52 subjects categorized into hyperglycemic (n=30) and normal subjects (n=22). Lipid species were determined from the following classes: cholesterol esters (CE), ceramides (CER), diacylglycerols (DAG), dihydroceramide (DCER), free fatty acids (FFA), hexosylceramides (HCER), lactosylceramides (LCER), lysophosphatidylcholines (LPC), lysophosphatidylethanolamines (LPE), phosphatidylcholines (PC), phosphatidylethanolamines (LPE), sphingomyelins (SM), and triacylglycerols (TAG). After normalizing by the sum of total apolipoprotein A and apolipoprotein B (to account for the possibility of reduced particle number due to medicinal intervention), lipids both by class and species were compared using one way analysis of variance. On a class level, relative to normal subjects, hyperglycemic patients showed increased levels of CER and FFA. Individual lipid species of these classes that were higher in hyperglycemic patients include CER(18:0), CER(20:0), CER(24:1), FFA(16:0), FFA(16:1), FFA(18:0), FFA(18:1), FFA(18:2), FFA(18:3), and FFA(20:3) Differences were also found in SM concentrations between hyperglycemic and normal patients resulting in a higher the SM/PC ratio, indicating changes in the lipid fluidity of the shell in lipoprotein particles of hyperglycemic patients. On a species level, SM(16:0), SM(18:1), and SM(24.1), were elevated in hyperglycemic subjects relative to normal subjects. This finding of elevated unsaturated SM species in hyperglycemic patients is at odds with other studies where it was found that the saturated SM moieties were elevated in insulin resistant subjects.

Simultaneous measurement of plasma apolipoproteins A-I and B by electroimmunoassay.

1982 ◽  
Vol 28 (1) ◽  
pp. 59-62 ◽  
Author(s):  
J C Fruchart ◽  
I Kora ◽  
C Cachera ◽  
V Clavey ◽  
P Duthilleul ◽  
...  
Keyword(s):  
Simultaneous Measurement ◽  
Apolipoprotein B ◽  
Secondary Standard ◽  
Plastic Film ◽  
Standard Curve ◽  
Atherosclerotic Lesions ◽  
Apolipoprotein A ◽  
Coefficients Of Variation

Abstract We describe a simplified electroimmunoassay for quantification of human apolipoproteins A-I and B on prepared plates. A solution of agarose at 55 degrees C, containing hydroxyethylcellulose and antibodies, is poured onto a plastic film and allowed to gel. Wells are punched in the gels and the plates are dried for storage. Before use, they are rehydrated and buffered. We use succinylated Sudan Black to prestain lipoprotein fractions of plasma. After electrophoresing samples of plasma or standards for 3 h at 4 degrees C at 12.5 V/cm, we measure the peak heights and read the results from a standard curve prepared by using calibrated sera of known apolipoprotein B and A-I content as secondary standard. The within- and between-assay coefficients of variation were less than 4% in all cases. Results correlated well with those obtained by classic electroimmunodiffusion. Subjects with confirmed atherosclerotic lesions had significantly (p less than 10(-9)) lower ratios of apolipoprotein A-I to apolipoprotein B, compared with ratios in controls.

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