scholarly journals Enzymes and related proteins as cancer biomarkers: A proteomic approach

2007 ◽  
Vol 381 (1) ◽  
pp. 93-97 ◽  
Author(s):  
Shu-Ling Liang ◽  
Daniel W. Chan
Keyword(s):  
Cancer Biomarkers ◽  
Proteomic Approach ◽  
Related Proteins

Comparative whey proteome analysis of small-tailed Han and DairyMeade ovine milk

2021 ◽  
pp. 1-5
Author(s):  
Urhan Bai ◽  
Xiaohu Su ◽  
Zhong Zheng ◽  
Liguo Zhang ◽  
Ying Ma ◽  
...  
Keyword(s):  
Mammary Gland Development ◽  
Proteome Analysis ◽  
Dairy Sheep ◽  
Milk Traits ◽  
Beneficial Effects ◽  
Proteomic Approach ◽  
Tandem Mass Tag ◽  
Proteome Profile ◽  
Related Proteins ◽  
Gland Development

Abstract We characterized the proteome profile of mid-lactation small-tailed Han (STH) and DairyMeade (DM) ovine milk in order to explore physiological variation and differences in milk traits between the two breeds. Methodology combined a tandem mass tag (TMT) proteomic approach with LC-MS/MS technology. A total of 656 proteins were identified in STH and DM ovine milk, of which 17and 29 proteins were significantly upregulated (P < 0.05) in STH and DM, respectively. Immune-related proteins and disease-related proteins were highly expressed in STH milk, whereas S100A2 and AEBP1 were highly expressed in DM milk, which had beneficial effects on mammary gland development and milk yield. Our results provide a theoretical basis for future breeding of dairy sheep.

scholarly journals IDENTIFICATION OF OXIDATIVE STRESS-RELATED PROTEINS FOR PREDICTIVE SCREENING OF HEPATOTOXICITY USING A PROTEOMIC APPROACH

2005 ◽  
Vol 30 (3) ◽  
pp. 213-227 ◽  
Author(s):  
Toshinori YAMAMOTO ◽  
Rie KIKKAWA ◽  
Hiroshi YAMADA ◽  
Ikuo HORII
Keyword(s):  
Oxidative Stress ◽  
Proteomic Approach ◽  
Related Proteins

scholarly journals Proteomic Analysis of Fusarium oxysporum-Induced Mechanism in Grafted Watermelon Seedlings

2021 ◽  
Vol 12 ◽  
Author(s):  
Man Zhang ◽  
Jinhua Xu ◽  
Runsheng Ren ◽  
Guang Liu ◽  
Xiefeng Yao ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Fusarium Oxysporum ◽  
Proteomic Analysis ◽  
Disease Incidence ◽  
Two Dimensional Gel Electrophoresis ◽  
Proteomic Approach ◽  
Scavenging Capacity ◽  
Pathogenesis Related ◽  
Functional Implications ◽  
Related Proteins

Grafting can improve the resistance of watermelon to soil-borne diseases. However, the molecular mechanism of defense response is not completely understood. Herein, we used a proteomic approach to investigate the molecular basis involved in grafted watermelon leaf defense against Fusarium oxysporum f.sp. niveum (FON) infection. The bottle gourd rootstock-grafted (RG) watermelon seedlings were highly resistant to FON compared with self-grafted (SG) watermelon plants, with a disease incidence of 3.4 and 89%, respectively. Meanwhile, grafting significantly induced the activity of pathogenesis-related proteases under FON challenge. Proteins extracted from leaves of RG and SG under FON inoculation were analyzed using two-dimensional gel electrophoresis. Thirty-nine differentially accumulated proteins (DAPs) were identified and classified into 10 functional groups. Accordingly, protein biosynthetic and stress- and defense-related proteins play crucial roles in the enhancement of disease resistance of RG watermelon seedlings, compared with that of SG watermelon seedlings. Proteins involved in signal transduction positively regulated the defense process. Carbohydrate and energy metabolism and photosystem contributed to energy production in RG watermelon seedlings under FON infection. The disease resistance of RG watermelon seedlings may also be related to the improved scavenging capacity of reactive oxygen species (ROS). The expression profile of 10 randomly selected proteins was measured using quantitative real-time PCR, among which, 7 was consistent with the results of the proteomic analysis. The functional implications of these proteins in regulating grafted watermelon response against F. oxysporum are discussed.

scholarly journals A Differential Proteomic Approach to Characterize the Cell Wall Adaptive Response to CO2 Overpressure during Sparkling Wine-Making Process

2020 ◽  
Vol 8 (8) ◽  
pp. 1188
Author(s):  
Juan Antonio Porras-Agüera ◽  
Juan Carlos Mauricio ◽  
Jaime Moreno-García ◽  
Juan Moreno ◽  
Teresa García-Martínez
Keyword(s):  
Cell Wall ◽  
Adaptive Response ◽  
Wine Yeast ◽  
Sparkling Wine ◽  
Cell Wall Proteins ◽  
Protein Biomarkers ◽  
Wine Making ◽  
Proteomic Approach ◽  
Flor Yeast ◽  
Related Proteins

In this study, a first proteomic approach was carried out to characterize the adaptive response of cell wall-related proteins to endogenous CO2 overpressure, which is typical of second fermentation conditions, in two wine Saccharomyces cerevisiae strains (P29, a conventional second fermentation strain, and G1, a flor yeast strain implicated in sherry wine making). The results showed a high number of cell wall proteins in flor yeast G1 under pressure, highlighting content at the first month of aging. The cell wall proteomic response to pressure in flor yeast G1 was characterized by an increase in both the number and content of cell wall proteins involved in glucan remodeling and mannoproteins. On the other hand, cell wall proteins responsible for glucan assembly, cell adhesion, and lipid metabolism stood out in P29. Over-represented proteins under pressure were involved in cell wall integrity (Ecm33p and Pst1p), protein folding (Ssa1p and Ssa2p), and glucan remodeling (Exg2p and Scw4p). Flocculation-related proteins were not identified under pressure conditions. The use of flor yeasts for sparkling wine elaboration and improvement is proposed. Further research based on the genetic engineering of wine yeast using those genes from protein biomarkers under pressure alongside the second fermentation in bottle is required to achieve improvements.

scholarly journals Protein Alterations in Infiltrating Ductal Carcinomas of the Breast as Detected by Nonequilibrium pH Gradient Electrophoresis and Mass Spectrometry

2008 ◽  
Vol 2008 ◽  
pp. 1-10 ◽  
Author(s):  
Maria Kabbage ◽  
Karim Chahed ◽  
Bechr Hamrita ◽  
Christelle Lemaitre Guillier ◽  
Mounir Trimeche ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Kinase Inhibitor ◽  
Cancer Biomarkers ◽  
Ph Gradient ◽  
Complement C3 ◽  
Gradient Electrophoresis ◽  
Wide Range ◽  
Ductal Carcinomas ◽  
Protein Alterations ◽  
Related Proteins

Improvement of breast-cancer detection through the identification of potential cancer biomarkers is considered as a promising strategy for effective assessment of the disease. The current study has used nonequilibrium pH gradient electrophoresis with subsequent analysis by mass spectrometry to identify protein alterations in invasive ductal carcinomas of the breast from Tunisian women. We have identified multiple protein alterations in tumor tissues that were picked, processed, and unambiguously assigned identities by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF). The proteins identified span a wide range of functions and are believed to have potential clinical applications as cancer biomarkers. They include glycolytic enzymes, molecular chaperones, cytoskeletal-related proteins, antioxydant enzymes, and immunologic related proteins. Among these proteins, enolase 1, phosphoglycerate kinase 1, deoxyhemoglobin, Mn-superoxyde dismutase,α-B-crystallin, HSP27, Raf kinase inhibitor protein, heterogeneous nuclear ribonucleoprotein A2/B1, cofilin 1, and peptidylprolyl isomerase A were overexpressed in tumors compared with normal tissues. In contrast, the IGHG1 protein, the complement C3 component C3c, which are two newly identified protein markers, were downregulated in IDCA tissues.

scholarly journals Physiological Responses and Proteomic Analysis on the Cold Stress Responses of Annual Pitaya (Hylocereus spp.) Branches

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Junliang Zhou ◽  
Lijuan Wang ◽  
Tujian Xiao ◽  
Zhuang Wang ◽  
Yongya Mao ◽  
...  
Keyword(s):  
Cold Stress ◽  
Stress Responses ◽  
Molecular Mechanisms ◽  
Cold Resistance ◽  
Physiological Responses ◽  
Soluble Sugar ◽  
Multivariate Statistical ◽  
Chlorophyll Contents ◽  
Proteomic Approach ◽  
Related Proteins

In this study, the physiological response of the annual branches of three varieties of pitaya (Xianmi, Fulong, and Zihonglong) in cold stress was investigated using a multivariate statistical method. Physiological change results showed that cold stress could decrease the moisture and chlorophyll contents, on the contrary, increase the relative electric conductivity, the contents of malonadehyde, soluble protein, soluble sugar, and free proline, and enhance the enzyme activities of peroxidase, superoxide dismutase, and catalase. Meanwhile, a comparative proteomic approach was also conducted to clarify the cold resistance-related proteins and pathways in annual pitaya branches. Proteomics results concluded that the cold tolerance of annual pitaya branches could be improved by modulating autophagy. Therefore, we hypothesized that an increased autophagy ability may be an important characteristic of the annual pitaya branches in response to cold stress conditions. Our results provide a good understanding of the physiological responses and molecular mechanisms of the annual pitaya branches in response to cold stress.

scholarly journals Comparative proteome analysis of rat brain and coronary microvascular endothelial cells

2007 ◽  
pp. 159-168
Author(s):  
L Lu ◽  
PY Yang ◽  
Y-Ch Rui ◽  
H Kang ◽  
J Zhang ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Proteome Analysis ◽  
Microvascular Endothelial Cells ◽  
Ionization Time ◽  
Proteomic Approach ◽  
Microvascular Endothelial ◽  
Signal Transduction Proteins ◽  
Ph Range ◽  
Related Proteins ◽  
Dimensional Electrophoresis

The endothelium of different organs displays a remarkable heterogeneity, although it presents many common functional and morphological features. However, despite our knowledge of heterogeneity among endothelial cells from different sites, the differences between brain microvascular endothelial cells (BMEC) and coronary microvascular endothelial cells (CMEC) are poorly defined. The aim of this study was to investigate whether BMEC are distinct from CMEC at the protein level. Using the proteomic approach, we comparatively analyzed the proteome of cultured BMEC and CMEC. We reproducibly separated over 2000 polypeptides by using two-dimensional electrophoresis (2-DE) at pH range of 3-10. Using PDQuest software to process the 2-DE gel images, forty-seven protein spots were differentially expressed in the two-endothelial cells. Of these, thirty-five proteins are highly expressed in BMEC, whereas twelve proteins are highly expressed in CMEC. Fifteen proteins in BMEC and seven proteins in CMEC were identified with high confidence by matrix-associated laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS). Our data suggested that BMEC and CMEC were different in several aspects including cytokine and growth-related molecules, stress-related proteins, metabolic enzymes, signal transduction proteins and others. The identification of a set of proteins preferentially expressed in BMEC and CMEC provided new data on the heterogeneity of the endothelium.

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