A novel LEAP-2 in diploid hybrid fish (Carassius auratus cuvieri ♀ × Carassius auratus red var. ♂) confers protection against bacteria-stimulated inflammatory response

2020 ◽  
Vol 228 ◽  
pp. 108665
Author(s):  
Sheng-Wei Luo ◽  
Kai-Kun Luo ◽  
Shao-Jun Liu
Keyword(s):  
Inflammatory Response ◽  
Carassius Auratus ◽  
Diploid Hybrid ◽  
Hybrid Fish

Antibacterial and immunoregulatory activity of a novel hepcidin homologue in diploid hybrid fish (Carassius auratus cuvieri ♀ × Carassius auratus red var ♂)

2020 ◽  
Vol 98 ◽  
pp. 551-563 ◽  
Author(s):  
Sheng-Wei Luo ◽  
Zi-Ye Luo ◽  
Teng Yan ◽  
Kai-Kun Luo ◽  
Ping-Hui Feng ◽  
...  
Keyword(s):  
Carassius Auratus ◽  
Diploid Hybrid ◽  
Hybrid Fish

scholarly journals Cell fusion as the formation mechanism of unreduced gametes in the gynogenetic diploid hybrid fish

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Jing Wang ◽  
Qingfeng Liu ◽  
Kaikun Luo ◽  
Xuan Chen ◽  
Jun Xiao ◽  
...  
Keyword(s):  
Cell Fusion ◽  
Formation Mechanism ◽  
Unreduced Gametes ◽  
Diploid Hybrid ◽  
Gynogenetic Diploid ◽  
Hybrid Fish

Developmental potential of somatic and germ cells of hybrids between Carassius auratus females and Hemigrammocypris rasborella males

Zygote ◽  
2020 ◽  
Vol 28 (6) ◽  
pp. 470-481
Author(s):  
Yuki Naya ◽  
Tomoka Matsunaga ◽  
Yu Shimizu ◽  
Eisuke Takahashi ◽  
Fumika Shima ◽  
...  
Keyword(s):  
Germ Cells ◽  
Carassius Auratus ◽  
Hybrid Sterility ◽  
Primordial Germ Cells ◽  
Triploid Hybrid ◽  
Hybrid Cells ◽  
Diploid Hybrid ◽  
Blastula Stage ◽  
Developmental Potential ◽  
Diploid Cells

SummaryThe cause of hybrid sterility and inviability has not been analyzed in the fin-fish hybrid, although large numbers of hybridizations have been carried out. In this study, we produced allo-diploid hybrids by cross-fertilization between female goldfish (Carassius auratus) and male golden venus chub (Hemigrammocypris rasborella). Inviability of these hybrids was due to breakage of the enveloping layer during epiboly or due to malformation with serious cardiac oedema around the hatching stage. Spontaneous allo-triploid hybrids with two sets of the goldfish genome and one set of the golden venus chub genome developed normally and survived beyond the feeding stage. This improved survival was confirmed by generating heat-shock-induced allo-triploid hybrids that possessed an extra goldfish genome. When inviable allo-diploid hybrid cells were transplanted into goldfish host embryos at the blastula stage, these embryos hatched normally, incorporating the allo-diploid cells. These allo-diploid hybrid cells persisted, and were genetically detected in a 6-month-old fish. In contrast, primordial germ cells taken from allo-diploid hybrids and transplanted into goldfish hosts at the blastula stage had disappeared by 10 days post-fertilization, even under chimeric conditions. In allo-triploid hybrid embryos, germ cells proliferated in the gonad, but had disappeared by 10 weeks post-fertilization. These results showed that while hybrid germ cells are inviable even in chimeric conditions, hybrid somatic cells remain viable.

scholarly journals Characterization of the acute inflammatory response in the hybrid tambacu (Piaractus mesopotamicus male × Colossoma macropomum female) (Osteichthyes)

2009 ◽  
Vol 69 (3) ◽  
pp. 957-962 ◽  
Author(s):  
ML. Martins ◽  
DMY. Myiazaki ◽  
M. Tavares-Dias ◽  
J. Fenerick Jr. ◽  
EM. Onaka ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Granular Cell ◽  
Native Fish ◽  
Acute Inflammatory Response ◽  
Inflammatory Exudate ◽  
Hybrid Fish ◽  
Inflammatory Site

This work evaluated the acute inflammatory response induced by injections of 0.5 mL saline solution (control), 500 µg carrageenin and 0.5 mL thioglycollate 3% in the swim bladder of juvenile tambacu hybrid. Fish were distributed in three treatments, three replications and acclimated for a period of 10 days before assay. The cell characterization from the inflammatory exudate was performed in Giemsa and PAS stained smears. Carrageenin, injected in fish, showed an increase on the total number of cells in the inflammatory exudate when compared to saline and thioglycollate injected. Whereas, for carrageenin-injected fish, the percentage of thrombocyte was higher than thioglycollate. On the other hand, granulocyte percentage in thioglycollate-injected fish was higher than the ones injected using carrageenin. Carrageenin provoked the highest migration of macrophage to the inflammatory site. The PAS method confirmed the presence of three types of granulocytes: eosinophilic granular cell (EGC) type 1 with the characteristics of a special granulocytic cell commonly found in the circulating blood; EGC type 2 shorter than the last one and neutrophil. This study contributes to a better understanding of the inflammatory response and infectious processes in native fish.

scholarly journals Diploid hybrid fish derived from the cross between female Bleeker’s yellow tail and male topmouth culter, two cyprinid fishes belonging to different subfamilies

BMC Genetics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Shengnan Li ◽  
Lihua Xie ◽  
Jun Xiao ◽  
Liujiao Yuan ◽  
Tian Zhou ◽  
...  
Keyword(s):  
5S Rdna ◽  
Reproductive Traits ◽  
Gonadal Development ◽  
Diploid Hybrid ◽  
Distant Hybridization ◽  
High Quality ◽  
Genetic Breeding ◽  
Fertile Female ◽  
Hybrid Fish ◽  
Fish Genetic Breeding

Abstract Background Bleeker’s yellow tail (Xenocypris davidi Bleeker, YT) and topmouth culter (Culter alburnus Basilewsky, TC) are both famous and important economic freshwater fish in China. YT, a kind of omnivorous fish, has strong resistance. TC, a kind of carnivorous fish, has high-quality meat but poor resistance. Distant hybridization can integrate the advantages of both parents. There has been no previous report regarding hybrid fish derived from female YT × male TC. It is expected that hybridization of these two kinds of fish will result in F1 hybrids with improved characteristics, such as faster growth rate, stronger resistance, and high-quality meat, which are of great significance in fish genetic breeding. Results In this study, we investigated the main biological characteristics of diploid hybrid fish derived from female YT × male TC. The hybrids had an intermediate number of upper lateral line scales between those for YT and TC. The hybrids were diploids with 48 chromosomes and had the same karyotype formula as their parents. The hybrids generated variations in 5S rDNA (designated class IV: 212 bp) and lost specific 5S rDNA derived from the maternal parent (designated class II: 221 bp), which might be related to hybridization. In terms of reproductive traits, all the tested female hybrids exhibited normal gonadal development, and the two-year-old F1 females produced mature eggs. However, all the tested testes of the male hybrids could not produce mature sperm. It is possible that the hybrid lineage will be established by back-crossing the fertile female hybrids and their parents. Conclusions Obtaining a fertile female hybrid fish made the creation of a new type of fish possible, which was significant in fish genetic breeding.

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