The Multixenobiotic resistance system as a possible protective response triggered by microplastic ingestion in Mediterranean mussels (Mytilus galloprovincialis): Larvae and adult stages

2019 ◽  
Vol 219 ◽  
pp. 50-58 ◽  
Author(s):  
Silvia Franzellitti ◽  
Marco Capolupo ◽  
Rajapaksha H.G.R. Wathsala ◽  
Paola Valbonesi ◽  
Elena Fabbri
Keyword(s):  
Mytilus Galloprovincialis ◽  
Protective Response ◽  
Multixenobiotic Resistance ◽  
Resistance System

scholarly journals Induction of the multixenobiotic/multidrug resistance system in various cell lines in response to perfluorinated carboxylic acids.

2008 ◽  
Vol 55 (2) ◽  
pp. 329-337 ◽  
Author(s):  
Izabela Rusiecka ◽  
Andrzej C Składanowski
Keyword(s):  
Flow Cytometry ◽  
Multidrug Resistance ◽  
Carboxylic Acids ◽  
Cell Lines ◽  
Rhodamine B ◽  
Efflux Pump ◽  
Measuring System ◽  
Multixenobiotic Resistance ◽  
Resistance System ◽  
Perfluorinated Carboxylic Acids

The multixenobiotic resistance (closely related to multidrug resistance) system controls transport across the plasma membrane as a defense against toxic molecules. Multixenobiotic resistance system consists of an efflux pump, ABCB1 (also named P-glycoprotein, P-gp), and/or a molecule of the ABCC family (also named multiple resistance associated protein, MRP). ABCB1 is able to increase efflux of many low-molecular foreign molecules. Measuring system induction may be used as a biomarker of cell/organism exposure to foreign substances. Various established cell lines were tested for constitutive and induced multixenobiotic resistance proteins by Western blotting immunodetection. The pumping function was indirectly assayed with Rhodamine B by visualization of cell fluorescence in the presence of verapamil. Changes in ABC proteins were measured by flow cytometry after exposition to various perfluorinated carboxylic acids. MCF7 and HeLa cells were found to contain the highest constitutive level of both ABCB1 and ABCC1. HEK293 exhibited much less ABCB1 and no activity of pumping out Rhodamine B. The pumping activity was found to be related to the amount of the cell-type specific 170 kDa ABCB1 protein. An 8-day exposure to 10(-4) M perfluorononanoic acid resulted in about 2-2.5-fold increase of ABCB1 level. That was confirmed also for short times by flow cytometry of cells exposed to perfluorinated acids and its natural congeners. Both ABCB1- and ABCC1-related fluorescence increased along with the carbon chain in acids from C(6) up to C(9) and decreased for C(10). Measuring of multixenobiotic resistance changes in vitro induced by chemicals may be a convenient test for screening for their potential toxicity.

scholarly journals Expression Analyses of Genes Related to Multixenobiotic Resistance in Mytilus galloprovincialis after Exposure to Okadaic Acid-Producing Dinophysis acuminate

Toxins ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 614
Author(s):  
Roi Martínez-Escauriaza ◽  
Vanessa Lozano ◽  
M. Luz Pérez-Parallé ◽  
Juan Blanco ◽  
José L. Sánchez ◽  
...  
Keyword(s):  
Digestive Gland ◽  
Okadaic Acid ◽  
Mytilus Galloprovincialis ◽  
Aquatic Organisms ◽  
Resistance Mechanisms ◽  
Multixenobiotic Resistance ◽  
Main Production ◽  
Detoxification Process ◽  
Mdr Genes

The mussel Mytilus galloprovincialis is one of the most important aquaculture species in Europe. Its main production problem is the accumulation of toxins during coastal blooms, which prevents mussel commercialization. P-glycoprotein (ABCB1/MDR1/P-gp) is part of the multixenobiotic resistance system in aquatic organisms, and okadaic acid, the main DSP toxin, is probably a substrate of the P-gp-mediated efflux. In this study, the presence and possible role of P-gp in the okadaic acid detoxification process was studied in M. galloprovincialis. We identified, cloned, and characterized two complete cDNAs of mdr1 and mdr2 genes. MgMDR1 and MgMDR2 predicted proteins had the structure organization of ABCB full transporters, and were identified as P-gp/MDR/ABCB proteins. Furthermore, the expression of mdr genes was monitored in gills, digestive gland, and mantle during a cycle of accumulation-elimination of okadaic acid. Mdr1 significantly increased its expression in the digestive gland and gills, supporting the idea of an important role of the MDR1 protein in okadaic acid efflux out of cells in these tissues. The expression of M. galloprovincialismrp2, a multidrug associated protein (MRP/ABCC), was also monitored. As in the case of mdr1, there was a significant induction in the expression of mrp2 in the digestive gland, as the content of okadaic acid increased. Thus, P-gp and MRP might constitute a functional defense network against xenobiotics, and might be involved in the resistance mechanisms to DSP toxins.

Expression and activity of a multixenobiotic resistance system in the Pacific oyster Crassostrea gigas

2002 ◽  
Vol 54 (3-5) ◽  
pp. 455-459 ◽  
Author(s):  
C Minier ◽  
C Lelong ◽  
N Djemel ◽  
F Rodet ◽  
R Tutundjian ◽  
...  
Keyword(s):  
Crassostrea Gigas ◽  
Pacific Oyster ◽  
Multixenobiotic Resistance ◽  
The Pacific ◽  
Resistance System ◽  
Expression And Activity
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