Exposure to low concentration of fluoxetine affects development, behaviour and acetylcholinesterase activity of zebrafish embryos

2019 ◽  
Vol 215 ◽  
pp. 1-8 ◽  
Author(s):  
Natália Oliveira de Farias ◽  
Rhaul Oliveira ◽  
Diego Sousa-Moura ◽  
Reginaldo Carlyle Silva de Oliveira ◽  
Maria Augusta Carvalho Rodrigues ◽  
...  
Keyword(s):  
Acetylcholinesterase Activity ◽  
Zebrafish Embryos ◽  
Low Concentration

Incorporation of biochemical endpoints to teratogenesis evaluation: Acetylcholinesterase activity in valporic acid treated zebrafish embryos

2010 ◽  
Vol 30 (2) ◽  
pp. 246-247
Author(s):  
Elisabet Teixidó ◽  
Ester Piqué ◽  
Jesús Gómez-Catalán ◽  
Joan M. Llobet
Keyword(s):  
Acetylcholinesterase Activity ◽  
Zebrafish Embryos

Segmental pattern of development of the hindbrain and spinal cord of the zebrafish embryo

Development ◽  
1988 ◽  
Vol 103 (1) ◽  
pp. 49-58 ◽  
Author(s):  
E. Hanneman ◽  
B. Trevarrow ◽  
W.K. Metcalfe ◽  
C.B. Kimmel ◽  
M. Westerfield
Keyword(s):  
Spinal Cord ◽  
Monoclonal Antibody ◽  
Early Development ◽  
Zebrafish Embryo ◽  
Single Cells ◽  
Acetylcholinesterase Activity ◽  
Zebrafish Embryos ◽  
Small Clusters ◽  
Spinal Motoneurones

In the ventral hindbrain and spinal cord of zebrafish embryos, the first neurones that can be identified appear as single cells or small clusters of cells, distributed periodically at intervals equal to the length of a somite. In the hindbrain, a series of neuromeres of corresponding length is present, and the earliest neurones are located in the centres of each neuromere. Young neurones within both the hindbrain and spinal cord were identified in live embryos using Nomarski optics, and histochemically by labelling for acetylcholinesterase activity and expression of an antigen recognized by the monoclonal antibody zn-1. Among them are individually identified hindbrain reticulospinal neurones and spinal motoneurones. These observations suggest that early development in these regions of the CNS reflects a common segmental pattern. Subsequently, as more neurones differentiate, the initially similar patterning of the cells in these two regions diverges. A continuous longitudinal column of developing neurones appears in the spinal cord, whereas an alternating series of large and small clusters of neurones is present in the hindbrain.

Chemically Contaminated Eel Fed to Pregnant and Lactating Mouse Dams Causes Hyperactivity in Their Offspring

2016 ◽  
Vol 86 (1-2) ◽  
pp. 36-47 ◽  
Author(s):  
Imen Dridi ◽  
Nidhal Soualeh ◽  
Torsten Bohn ◽  
Rachid Soulimani ◽  
Jaouad Bouayed
Keyword(s):  
Open Field ◽  
Early Life ◽  
Home Cage ◽  
Anguilla Anguilla ◽  
Acetylcholinesterase Activity ◽  
Female Offspring ◽  
Significant Inhibition ◽  
Standard Diet ◽  
Early Life Exposure ◽  
Cage Test

Abstract.This study examined whether perinatal exposure to polluted eels (Anguilla anguilla L.) induces changes in the locomotor activity of offspring mice across lifespan (post-natal days (PNDs) 47 – 329), using the open field and the home cage activity tests. Dams were exposed during gestation and lactation, through diets enriched in eels naturally contaminated with pollutants including PCBs. Analysis of the eel muscle focused on the six non-dioxin-like (NDL) indicator PCBs (Σ6 NDL-PCBs: 28, 52, 101, 138, 153 and 180). Four groups of dams (n = 10 per group) received either a standard diet without eels or eels (0.8 mg/kg/day) containing 85, 216, or 400 ng/kg/day of ϵ6 NDL-PCBs. The open field test showed that early-life exposure to polluted eels increased locomotion in female offspring of exposed dams but not in males, compared to controls. This hyperlocomotion appeared later in life, at PNDs 195 and 329 (up to 32 % increase, p < 0.05). In addition, overactivity was observed in the home cage test at PND 305: exposed offspring females showed a faster overall locomotion speed (3.6 – 4.2 cm/s) than controls (2.9 cm/s, p <0.05); again, males remained unaffected. Covered distances in the home cage test were only elevated significantly in offspring females exposed to highest PCB concentrations (3411 ± 590 cm vs. 1377 ± 114 cm, p < 0.001). These results suggest that early-life exposure to polluted eels containing dietary contaminants including PCBs caused late, persistent and gender-dependent neurobehavioral hyperactive effects in offspring mice. Furthermore, female hyperactivity was associated with a significant inhibition of acetylcholinesterase activity in the hippocampus and the prefrontal cortex.

Mapping of accumulation of SeNPs in developing zebrafish embryos and larvae: a new method using SEM with energy dispersive X‐ray spectrometer

2017 ◽  
Vol 12 (7) ◽  
pp. 497-499
Author(s):  
Kalimuthu Kalishwaralal ◽  
Subhaschandrabose Jeyabharathi ◽  
Krishnan Sundar ◽  
Azhaguchamy Muthukumaran
Keyword(s):  
Energy Dispersive ◽  
New Method ◽  
Zebrafish Embryos ◽  
X Ray

Normal phase HPLC profiling of the acetylcholinesterase activity in apolar plant extracts

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
A Landreau ◽  
S Bertrand ◽  
C Simoes-Pires ◽  
L Marcourt ◽  
TD Bach ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Acetylcholinesterase Activity ◽  
Normal Phase ◽  
Normal Phase Hplc
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