scholarly journals Investigation of the potential endocrine effect of nitrate in zebrafish Danio rerio and brown trout Salmo trutta

Author(s):  
Poul Bjerregaard ◽  
Karin Lund Kinnberg ◽  
Maria Pedersen Mose ◽  
Henrik Holbech
2020 ◽  
Vol 7 (3) ◽  
pp. 31-40
Author(s):  
Yu. P. Rud ◽  
M. I. Maistrenko ◽  
O. V. Zaloilo ◽  
G. A. Liubchenko ◽  
L. P. Buchatskiy ◽  
...  

Aim. Although more than 80 fi sh species and some invertebrate aquatic organisms are known to be susceptible to in- fectious pancreatic necrosis virus (IPNV) and aquatic birnaviruses, relatively little is known about its ability to infect brown trout (Salmo trutta), zebrafi sh (Danio rerio), and a mollusk, swan mussel (Anodonta cygnea). Therefore, the aim was to study in more detail the effects of IPNV on the said organisms. The information on the virulence of birnaviruses for aquatic organisms is important for better understanding of their transmission and for the identifi cation of suscep- tible or transient (symptomless) hosts. Methods. Cell culture method, using RTG-2 cells, was used for in vitro culti- vation of IPNV as well as virus titer assessment. The supernatants from infected RTG-2 cells were used for artifi cial inoculation to obtain experimental infection of the fi sh and mollusk. Fish and mollusk behaviour and mortality were monitored daily. The infectious titer of the virus in experimental animals was assessed by cell culture assay and reverse transcription PCR (RT-PCR). Results. This paper presents the results of IPNV experimental infection of brown trout (Salmo trutta), zebrafi sh (Danio rerio), and an aquatic invertebrate mollusk - swan mussel (Anodonta cygnea). The mortality of brown trout and zebrafi sh was 76.6 and 40 % respectively. Swan mussel was apparently resistant to the virus, but it apparently accumulated and maintained substantial levels (up to 10 -10 ID 50 /ml -1 ) of the virus for the dura- tion of the experiment (35 days). The highest infectious titer was found in brown trout (10 6 ID 50 /ml -1 ). In zebrafi sh and swan mussels, it was up to 10 4.5 ID 50 /ml -1 and 10 2 ID 50 /ml -1 respectively. Conclusions. At the end of the experiments the IPNV titers were higher than the initial viral doses in brown trout and zebrafi sh, indicating virus reproduction. In diseasesd fi sh the blackening, whirling and anorexia were observed. It was shown that swan mussel accumulates and maintains virus for at least 35 days under experimental conditions and that it may act as symptomless vector of IPNV


2013 ◽  
Vol 144-145 ◽  
pp. 19-25 ◽  
Author(s):  
Henrik Holbech ◽  
Kristoffer D. Schröder ◽  
Marie L. Nielsen ◽  
Nanna Brande-Lavridsen ◽  
Bente Frost Holbech ◽  
...  

2011 ◽  
Vol 14 (3) ◽  
pp. 443-448 ◽  
Author(s):  
N. Kurhalyuk ◽  
H. Tkachenko ◽  
K. Pałczyńska

Resistance of erythrocytes from Brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis syndrome In the present work we evaluated the effect of ulcerative dermal necrosis (UDN) syndrome on resistance of erythrocytes to haemolytic agents and lipid peroxidation level in the blood from brown trout (Salmo trutta m. trutta L.). Results showed that lipid peroxidation increased in erythrocytes, as evidenced by high thiobarbituric acid reactive substance (TBARS) levels. Compared to control group, the resistance of erythrocytes to haemolytic agents was significantly lower in UDN-positive fish. Besides, UDN increased the percent of hemolysated erythrocytes subjected to the hydrochloric acid, urea and hydrogen peroxide. Results showed that UDN led to an oxidative stress in erythrocytes able to induce enhanced lipid peroxidation level, as suggested by TBARS level and decrease of erythrocytes resistance to haemolytic agents.


2021 ◽  
Vol 29 ◽  
pp. 100675
Author(s):  
Muhammed Atamanalp ◽  
Arzu Ucar ◽  
Esat Mahmut Kocaman ◽  
Gonca Alak

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