scholarly journals Assaying uptake of endocrine disruptor compounds in zebrafish embryos and larvae

2018 ◽  
Vol 208 ◽  
pp. 105-113 ◽  
Author(s):  
J. Paige Souder ◽  
Daniel A. Gorelick
Keyword(s):  
Endocrine Disruptor ◽  
Zebrafish Embryos ◽  
Endocrine Disruptor Compounds

Bi-enzymatic virus-like bionanoreactors for the transformation of endocrine disruptor compounds

2020 ◽  
Vol 146 ◽  
pp. 415-421 ◽  
Author(s):  
Oscar González-Davis ◽  
Kanchan Chauhan ◽  
Santino-Jesuín Zapian-Merino ◽  
Rafael Vazquez-Duhalt
Keyword(s):  
Endocrine Disruptor ◽  
Endocrine Disruptor Compounds

scholarly journals Modeling and optimization of a photocatalytic process: Degradation of endocrine disruptor compounds by Ag/ZnO

2017 ◽  
Vol 128 ◽  
pp. 174-191 ◽  
Author(s):  
Alma Berenice Jasso-Salcedo ◽  
Sandrine Hoppe ◽  
Fernand Pla ◽  
Vladimir Alonso Escobar-Barrios ◽  
Mauricio Camargo ◽  
...  
Keyword(s):  
Endocrine Disruptor ◽  
Photocatalytic Process ◽  
Modeling And Optimization ◽  
Endocrine Disruptor Compounds

scholarly journals Assaying uptake of endocrine disruptor compounds in zebrafish embryos and larvae

2017 ◽  
Author(s):  
J. Paige Souder ◽  
Daniel A. Gorelick
Keyword(s):  
Bisphenol A ◽  
Endocrine Disruptors ◽  
Cyclosporine A ◽  
Abc Transporters ◽  
Endocrine Disruptor ◽  
Ethinyl Estradiol ◽  
Aquatic Organisms ◽  
Zebrafish Embryos ◽  
Environmental Endocrine Disruptors

AbstractTo study the effects of environmental endocrine disruptors (EEDs) on aquatic animals, embryos and larvae are typically incubated in water containing defined concentrations of EEDs. However, the amount of EED uptake into the animal is often difficult to determine. Using radiolabeled estradiol ([3H]E2), we previously developed a rapid, straightforward assay to measure estradiol uptake from water into zebrafish embryos and larvae. Here, we extend this approach to measure the uptake of two additional EEDs, bisphenol A (BPA) and ethinyl estradiol (EE2). As with E2, the uptake of each compound by individual larvae was low (< 6%), and increased with increasing concentration, duration, and developmental stage. We found that E2 and EE2 had similar uptake under equivalent exposure conditions, while BPA had comparatively lower uptake. One application of this assay is to test factors that influence EED uptake or efflux. It has been suggested that persistent organic pollutants (POPs) inhibit ABC transporters that may normally efflux EEDs and their metabolites, inducing toxicity in aquatic organisms. We measured [3H]E2 levels in zebrafish in the presence or absence of the POP PDBE-100, and cyclosporine A, a known inhibitor of ABC transporters. Neither chemical significantly affected [3H]E2 levels in zebrafish, suggesting that zebrafish maintain estradiol efflux in the presence of PDBE-100, independently of cyclosporine A-responsive transporters. These uptake results will be a valuable reference for EED exposure studies in developing zebrafish, and provide a rapid assay to screen for chemicals that influence estrogen-like EED levels in vivo.

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