Reactive oxygen species and anti-oxidant defenses in tail of tadpoles, Xenopus laevis

2013 ◽  
Vol 158 (2) ◽  
pp. 101-108 ◽  
Author(s):  
Jennifer Johnson ◽  
William Manzo ◽  
Eileen Gardner ◽  
Jaishri Menon
Keyword(s):  
Reactive Oxygen Species ◽  
Xenopus Laevis ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Anti Oxidant

Effect of reactive oxygen species on NH 4 + permeation in Xenopus laevis oocytes

2002 ◽  
Vol 282 (6) ◽  
pp. C1445-C1453 ◽  
Author(s):  
Marc Cougnon ◽  
Samia Benammou ◽  
Franck Brouillard ◽  
Philippe Hulin ◽  
Gabrielle Planelles
Keyword(s):  
Reactive Oxygen Species ◽  
Xenopus Laevis ◽  
Reactive Oxygen ◽  
Membrane Conductance ◽  
Xenopus Laevis Oocytes ◽  
Oxygen Species ◽  
Cation Conductance ◽  
Β Amyloid ◽  
Oocyte Membrane ◽  
Ros Scavengers

To investigate the effects of reactive oxygen species (ROS) on NH[Formula: see text]permeation in Xenopus laevis oocytes, we used intracellular double-barreled microelectrodes to monitor the changes in membrane potential ( V m) and intracellular pH (pHi) induced by a 20 mM NH4Cl-containing solution. Under control conditions, NH4Cl exposure induced a large membrane depolarization (to V m = 4.0 ± 1.5 mV; n = 21) and intracellular acidification [reaching a change in pHi(ΔpHi) of 0.59 ± 0.06 pH units in 12 min]; the initial rate of cell acidification (dpHi/d t) was 0.06 ± 0.01 pH units/min. Incubation of the oocytes in the presence of H2O2 or β-amyloid protein had no marked effect on the NH4Cl-induced ΔpHi. By contrast, in the presence of photoactivated rose bengal (RB), tert-butyl-hydroxyperoxide ( t-BHP), or xanthine/xanthine oxidase (X/XO), the same experimental maneuver induced significantly greater ΔpHi and dpHi/d t. These increases in ΔpHiand dpHi/d t were prevented by the ROS scavengers histidine and desferrioxamine, suggesting involvement of the reactive species 1ΔgO2 and ·OH. Using the voltage-clamp technique to identify the mechanism underlying the ROS-measured effects, we found that RB induced a large increase in the oocyte membrane conductance ( G m). This RB-induced G m increase was prevented by 1 mM diphenylamine-2-carboxylate (DPC) and by a low Na+concentration in the bath. We conclude that RB, t-BHP, and X/XO enhance NH[Formula: see text] influx into the oocyte via activation of a DPC-sensitive nonselective cation conductance pathway.

LOWER EXPRESSION OF ANTI-OXIDANT ENZYMES IN HUMAN BETA CELL ACCOUNTS FOR THE VULNERABILITY TO REACTIVE OXYGEN SPECIES

2008 ◽  
Vol 86 (Supplement) ◽  
pp. 448
Author(s):  
A Miki ◽  
C Ricordi ◽  
A Mita ◽  
S Barker ◽  
R-D Molano ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Beta Cell ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Anti Oxidant ◽  
Human Beta Cell ◽  
Lower Expression

MP44-02 BASELINE LEVELS OF SEMINAL REACTIVE OXYGEN SPECIES PREDICT IMPROVEMENTS IN SPERM FUNCTION FOLLOWING ANTI-OXIDANT THERAPY IN MEN WITH INFERTILITY

2020 ◽  
Vol 203 ◽  
pp. e656
Author(s):  
Tharu Tharakan* ◽  
Wayne Vessey ◽  
Cassandra McDonald ◽  
Paula Almeida ◽  
Monica Figueiredo ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Sperm Function ◽  
Oxygen Species ◽  
Anti Oxidant ◽  
Baseline Levels

Loss of Foxo3a In FA Mice Leads to Hematopoietic Stem Cell Exhaustion That Can Be Attenuated by Natural Anti-Oxidant Quercetin.

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 1166-1166
Author(s):  
Jie Li ◽  
Jared Sipple ◽  
Qishen Pang
Keyword(s):  
Reactive Oxygen Species ◽  
Progenitor Cells ◽  
Peripheral Blood ◽  
Conflicts Of Interest ◽  
Genetic Disorder ◽  
Specific Interaction ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Hematopoietic Stem ◽  
Anti Oxidant

Abstract Abstract 1166 Fanconi anemia (FA) is a genetic disorder characterized by genomic instability, bone marrow (BM) failure and predisposition to cancer. However, FA mouse models do not show spontaneous genetic instability. Previous study shows that FOXO3a is associated with the FA pathway through oxidative stress-specific interaction with FANCD2. To address the consequence of loss of FOXO3a function in FA hematopoiesis, we generated Foxo3a-/-Fancd2-/- and Foxo3a-/-Fancc-/- double-knockout (DKO) mice by crossing Foxo3a+/− with Fancd2+/− or Fancc+/−; mice. Reactive oxygen species are increased in low-density BM (LDBM) cells isolated from DKO mice compared to those from single KO (SKO) or wt mice. Analysis of hematologic parameters shows significantly increased number of nucleate cells and high ratio of eosinophils in peripheral blood of DKO mice. CFU assay shows more progenitor cells in peripheral blood isolated from DKO mice. Moreover, BM progenitor cells from DKO mice exhibit lower adhesion but higher migration activity, compared to those from wt or SKO mice. Consistent with this, Cdc42 pull-down assay shows lower Cdc42 activity in DKO LDBM cells than in wt or SKO cells, indicating that decreased Cdc42 may contribute to the observed aberrant adhesion and migration activities. DKO mice show significant decrease in primitive progenitor (Lin-Sca-1+c-kit+; LSK) cells, increase in BrdU+ and G1-phase LSK cells, and impaired repopulating capacity after competitive BM transplantation, which can be attenuated by the anti-oxidant Quercetin. Taken together, loss of Foxo3a in FA mice results in FA-like syndrome, which may be resulted from increased reactive oxygen species accumulation. Disclosures: No relevant conflicts of interest to declare.

JRK’S ANTI- COFF – AN EFFECTIVE REMEDY FOR REACTIVE OXYGEN SPECIES SCAVENGING

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Soundharya R ◽  
Aruna V ◽  
Amruthavalli GV ◽  
Gayathri R
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Therapeutic Benefit ◽  
Blood Purification ◽  
Ocimum Sanctum ◽  
Oxygen Species ◽  
Leucas Aspera ◽  
Anti Oxidant ◽  
Effective Remedy ◽  
Acalypha Indica

JRK’s Anti-coff was studied for reactive oxygen species scavenging benefit inorder to establish the anti-oxidant therapeutic benefit. Findings clearly show that the herbs used in JRK’s Anti-coff such as Anisochilus carnosus, Leucas aspera, Ocimum sanctum, Solanum trilobatum, Acalypha indica, and Adhatoda vasica showed antioxidant effect. We have attempted to co-relate the above benefit of JRK’s Anti-coff with the blood purification benefit postulated by Ayurveda and Siddha. Further the importance of blood purification for the treatment of several diseases is discussed. Keywords: Ocimum sanctum, ROS, Blood purification effect, JRK’s Anti- coff

Reactive oxygen species and anti-oxidant defences in swine follicular fluids

2008 ◽  
Vol 20 (2) ◽  
pp. 269 ◽  
Author(s):  
Giuseppina Basini ◽  
Bussolati Simona ◽  
Sujen Eleonora Santini ◽  
Francesca Grasselli
Keyword(s):  
Reactive Oxygen Species ◽  
Ovarian Follicle ◽  
Reactive Oxygen ◽  
Redox Status ◽  
Follicle Development ◽  
Oxygen Species ◽  
Detoxifying Enzymes ◽  
Anti Oxidant ◽  
Reduced Activity ◽  
Follicular Fluids

A growing body of evidence indicates that the pro-oxidant/anti-oxidant balance inside the ovarian follicle plays an important role in folliculogenesis. Therefore, the aim of the present study was to assess the redox status of follicular fluids collected from different-sized swine follicles. We quantified the most important reactive oxygen species (ROS), namely superoxide anion (O2–), hydrogen peroxide and hydroperoxides (ROOH); in addition, we examined the activity of the detoxifying enzymes superoxide dismutase, catalase (CAT) and glutathione peroxidase and the total non-enzymatic antioxidant capacity as determined by the ferric-reducing anti-oxidant power assay. Our data demonstrate that oxidative stress does not affect follicle growth because O2– levels do not change during follicle development, whereas concentrations of H2O2 and ROOH are reduced (P < 0.05). Surprisingly, all non-enzymatic and enzymatic scavengers examined in the present study, except for CAT, demonstrated reduced activity during follicle development (P < 0.05). Taken together, these results suggest that other factors could be involved in ROS detoxification during follicle development.

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