In vitro effects of bisphenol A on the quality parameters, oxidative stress, DNA integrity and adenosine triphosphate content in sterlet (Acipenser ruthenus) spermatozoa

2013 ◽  
Vol 158 (2) ◽  
pp. 64-71 ◽  
Author(s):  
Martin Hulak ◽  
Ievgeniia Gazo ◽  
Anna Shaliutina ◽  
Pavla Linhartova
Keyword(s):  
Oxidative Stress ◽  
Bisphenol A ◽  
Adenosine Triphosphate ◽  
Quality Parameters ◽  
Dna Integrity ◽  
Acipenser Ruthenus ◽  
In Vitro Effects ◽  
Sterlet Acipenser Ruthenus

Effects of tetrabrombisphenol A on DNA integrity, oxidative stress, and sterlet (Acipenser ruthenus) spermatozoa quality variables

2014 ◽  
Vol 30 (7) ◽  
pp. 735-745 ◽  
Author(s):  
Pavla Linhartova ◽  
Ievgeniia Gazo ◽  
Anna Shaliutina-Kolesova ◽  
Martin Hulak ◽  
Vojtech Kaspar
Keyword(s):  
Oxidative Stress ◽  
Dna Integrity ◽  
Acipenser Ruthenus ◽  
Sterlet Acipenser Ruthenus

Mutagenicity of bisphenol A (4,4?-isopropylidenediphenol) in vitro: effects of nitrosylation

2002 ◽  
Vol 22 (6) ◽  
pp. 425-441 ◽  
Author(s):  
Timothy J. Schrader ◽  
I. Langlois ◽  
K. Soper ◽  
W. Cherry
Keyword(s):  
Bisphenol A ◽  
In Vitro Effects

scholarly journals Aminoguanidine Protects Boar Spermatozoa against the Deleterious Effects of Oxidative Stress

Pharmaceutics ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 212 ◽  
Author(s):  
Eliana Pintus ◽  
Martin Kadlec ◽  
Marija Jovičić ◽  
Markéta Sedmíková ◽  
José Ros-Santaella
Keyword(s):  
Oxidative Stress ◽  
Therapeutic Agent ◽  
Antioxidant Properties ◽  
In Vitro Effects ◽  
Oxide Synthase ◽  
Boar Spermatozoa ◽  
Generating System ◽  
Control Samples ◽  
Inducible Nitric Oxide

Aminoguanidine is a selective inhibitor of the inducible nitric oxide synthase (iNOS) and a scavenger of reactive oxygen species (ROS). Numerous studies have shown the antioxidant properties of aminoguanidine in several cell lines, but the in vitro effects of this compound on spermatozoa under oxidative stress are unknown. In this study, we tested the hypothesis that aminoguanidine may protect against the detrimental effects of oxidative stress in boar spermatozoa. For this purpose, sperm samples were incubated with a ROS generating system (Fe2+/ascorbate) with or without aminoguanidine supplementation (10, 1, and 0.1 mM). Our results show that aminoguanidine has powerful antioxidant capacity and protects boar spermatozoa against the deleterious effects of oxidative stress. After 2 h and 3.5 h of sperm incubation, the samples treated with aminoguanidine showed a significant increase in sperm velocity, plasma membrane and acrosome integrity together with a reduced lipid peroxidation in comparison with control samples (p < 0.001). Interestingly, except for the levels of malondialdehyde, the samples treated with 1 mM aminoguanidine did not differ or showed better performance than control samples without Fe2+/ascorbate. The results from this study provide new insights into the application of aminoguanidine as an in vitro therapeutic agent against the detrimental effects of oxidative stress in semen samples.

scholarly journals Thyroid Hormones and Spermatozoa: In Vitro Effects on Sperm Mitochondria, Viability and DNA Integrity

2019 ◽  
Vol 8 (5) ◽  
pp. 756 ◽  
Author(s):  
Rosita A. Condorelli ◽  
Sandro La Vignera ◽  
Laura M. Mongioì ◽  
Angela Alamo ◽  
Filippo Giacone ◽  
...  
Keyword(s):  
Thyroid Hormones ◽  
Seminal Fluid ◽  
In Vitro Study ◽  
Sperm Parameters ◽  
Dna Integrity ◽  
Sperm Analysis ◽  
Idiopathic Infertility ◽  
In Vitro Effects

The aim of this study wasto assess the in vitro effects of levothyroxine (LT4) on conventional and bio-functional sperm parameters and its implications on fertility. Patients with male idiopathic infertility were enrolled and subjected to examination of the seminal fluid and capacitation according to the WHO 2010 criteria and flow cytometric sperm analysis for the evaluation of bio-functional sperm parameters. LT4 significantly increased the percentage of spermatozoa with high mitochondrial membrane potential (MMP), decreased the percentage of spermatozoa with low MMP and increased sperm motility already at a concentration of 0.9 pmol L−1. Therefore, LT4 significantly reduced sperm necrosis and lipid peroxidation ameliorating chromatin compactness. These effects of LT4 were evident at a concentration of 2.9 pmol L−1, close to the physiological free-thyroxine (FT4) concentrations in the seminal fluid of euthyroid subjects. We showed a beneficial role of thyroid hormones on sperm mitochondrial function, oxidative stress and DNA integrity. The results of this in vitro study could have a clinical application in patients with idiopathic infertility, clarifying the role of thyroid function on male fertility.

The In Vitro Effects of Propofol on Tissular Oxidative Stress in the Rat

1998 ◽  
Vol 87 (5) ◽  
pp. 1141-1146 ◽  
Author(s):  
J. P. De La Cruz ◽  
G. Sedeno ◽  
J. A. Carmona ◽  
F. Sanchez de la Cuesta
Keyword(s):  
Oxidative Stress ◽  
In Vitro Effects
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