scholarly journals Cryopreservation and in vitro culture of primary cell types from lung tissue of a stranded pygmy sperm whale (Kogia breviceps)

2012 ◽  
Vol 155 (1) ◽  
pp. 136-142 ◽  
Author(s):  
Annalaura Mancia ◽  
Demetri D. Spyropoulos ◽  
Wayne E. McFee ◽  
Danforth A. Newton ◽  
John E. Baatz
Keyword(s):  
In Vitro Culture ◽  
Lung Tissue ◽  
Sperm Whale ◽  
Cell Types ◽  
Primary Cell ◽  
Kogia Breviceps ◽  
Pygmy Sperm Whale

scholarly journals Induction of Apoptosis of Metastatic Mammary Carcinoma Cells In Vivo by Disruption of Tumor Cell Surface CD44 Function

1997 ◽  
Vol 186 (12) ◽  
pp. 1985-1996 ◽  
Author(s):  
Qin Yu ◽  
Bryan P. Toole ◽  
Ivan Stamenkovic
Keyword(s):  
Cell Surface ◽  
Tumor Cell ◽  
Tumor Cells ◽  
Lung Tissue ◽  
Mammary Carcinoma ◽  
Cell Types ◽  
Pulmonary Endothelium ◽  
Soluble Cd44

To understand how the hyaluronan receptor CD44 regulates tumor metastasis, the murine mammary carcinoma TA3/St, which constitutively expresses cell surface CD44, was transfected with cDNAs encoding soluble isoforms of CD44 and the transfectants (TA3sCD44) were compared with parental cells (transfected with expression vector only) for growth in vivo and in vitro. Local release of soluble CD44 by the transfectants inhibited the ability of endogenous cell surface CD44 to bind and internalize hyaluronan and to mediate TA3 cell invasion of hyaluronan-producing cell monolayers. Mice intravenously injected with parental TA3/St cells developed massive pulmonary metastases within 21–28 d, whereas animals injected with TA3sCD44 cells developed few or no tumors. Tracing of labeled parental and transfectant tumor cells revealed that both cell types initially adhered to pulmonary endothelium and penetrated the interstitial stroma. However, although parental cells were dividing and forming clusters within lung tissue 48 h following injection, >80% of TA3sCD44 cells underwent apoptosis. Although sCD44 transfectants displayed a marked reduction in their ability to internalize and degrade hyaluronan, they elicited abundant local hyaluronan production within invaded lung tissue, comparable to that induced by parental cells. These observations provide direct evidence that cell surface CD44 function promotes tumor cell survival in invaded tissue and that its suppression can induce apoptosis of the invading tumor cells, possibly as a result of impairing their ability to penetrate the host tissue hyaluronan barrier.

scholarly journals Efficient and simple approach to in vitro culture of primary epithelial cancer cells

2016 ◽  
Vol 36 (6) ◽  
Author(s):  
Karolina Janik ◽  
Marta Popeda ◽  
Joanna Peciak ◽  
Kamila Rosiak ◽  
Maciej Smolarz ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
In Vitro Culture ◽  
Cancer Cells ◽  
Simple Approach ◽  
Primary Cell ◽  
Epithelial Cancer ◽  
Extracellular Matrix Components ◽  
Matrix Components ◽  
Cell Medium

Primary breast and prostate epithelial cancer cells may be efficiently cultured in vitro using simple and easily validatable approach–plates coated with a mixture of extracellular matrix components and tissue-specific primary cell medium.

scholarly journals Struvite Penile Urethrolithiasis in a Pygmy Sperm Whale (Kogia breviceps)

2004 ◽  
Vol 40 (3) ◽  
pp. 588-593 ◽  
Author(s):  
Craig A. Harms ◽  
Rachel Lo Piccolo ◽  
David S. Rotstein ◽  
Aleta A. Hohn
Keyword(s):  
Sperm Whale ◽  
Kogia Breviceps ◽  
Pygmy Sperm Whale

223 DESCRIPTION OF GENITALIA AND SPERM RECOVERED POSTMORTEM FROM A PYGMY SPERM WHALE, KOGIA BREVICEPS

2006 ◽  
Vol 18 (2) ◽  
pp. 219
Author(s):  
F. Martinez-Pastor ◽  
V. Garcia-Macias ◽  
J. Garcia ◽  
M. Alvarez ◽  
E. Anel ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Plasma Membranes ◽  
Sperm Concentration ◽  
Rare Event ◽  
Sperm Whale ◽  
Testicular Tissue ◽  
Sperm Head ◽  
Similar Species ◽  
Kogia Breviceps ◽  
Pygmy Sperm Whale

A pygmy sperm whale (Kogia breviceps; adult male; 350 kg) was stranded and died on a beach near Cabo Bustos (Asturias, North of Spain) on March 12th, 2005. Finding specimens of this species is a rare event on Spanish shores, although this whale is not considered endangered. Postmortem examination was performed 24 h later. Genitalia (testicles and epididymides) were extracted. The postmortem report indicated that vas deferens and seminal glands seemed to contain an important amount of semen, which was not recovered. Refrigerated genitalia were send to our laboratory, arriving around 40 h postmortem. The refrigerated testicles were in poor physical condition upon arrival, indicating advanced tissue detoriation. The epididymides (very long) were not closely attached to the testicles, but were connected by a loose conjunctive membrane. We divided the epididymides into four regions that approximated the (1) caput, (2) mid-region, (3) corpus, and (4) cauda. Physical characteristics of the genitalia are described in Table 1. The left testicle was larger, and possibly more active, than the right one. A sperm sample was obtained from the cauda region after incising the tissue. Osmolality and pH of the sample were 428 mOsm/kg and 6.62, respectively (maybe due to tissue breakdown) and the sperm concentration was 1194 × 106/mL. Spermatozoa were immotile, even after diluting in buffered medium; it is possible that postmortem damage occurred quickly. However, using flow cytometry we determined that 57% of cauda spermatozoa had intact plasma membranes and acrosomes (determined by staining with 37 mmol/mL propidium iodide and 1 μg/mL PNA-FITC; Sigma, Madrid, Spain). Examination by phase contrast microscopy (×600) showed many spermatozoa with abnormal heads and bent midpieces and flagella, even in the cauda (13% and 21%, respectively). Sperm head morphometry was studied using DiffQuick staining and an automated analysis system (SCA2000; Microptic, Barcelona, Spain). Mean sperm head size was 3.71 ± 0.19 × 2.61 ± 0.12 μm in width and length, respectively. Computer analysis (AnalySiS-GmbH, Cologne, Germany) of phase contrast images revealed that the mean size of the sperm midpiece and flagellum were 3.44 ± 0.19 and 40.95 ± 2.02 μm, respectively. The information obtained after postmortem recovery of the testes and epididymis should be useful to future conservation efforts of the pygmy sperm whale and similar species. The rapid deterioration of the testicular tissue by 40 h postmortem was not expected since good quality sperm samples have been obtained at similar postmortem intervals in other species. Therefore, we recommend that postmortem sperm recovery should be accomplished as rapidly as possible in this species. Table 1. Genitalia measurements

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