Pulmonary cytochrome P450 enzymes belonging to the CYP4B subfamily from an Australian marsupial, the tammar wallaby (Macropus eugenii)

2011 ◽  
Vol 153 (1) ◽  
pp. 60-66 ◽  
Author(s):  
Natalie L. Milic ◽  
Suong N.T. Ngo ◽  
Ceilidh L. Marchant ◽  
Tamara A. Height ◽  
Ross A. McKinnon
Keyword(s):  
Cytochrome P450 ◽  
Tammar Wallaby ◽  
Cytochrome P450 Enzymes ◽  
Macropus Eugenii ◽  
Australian Marsupial

Mullerian inhibiting substance production and testicular migration and descent in the pouch young of a marsupial

Development ◽  
1988 ◽  
Vol 104 (4) ◽  
pp. 549-556 ◽  
Author(s):  
J.M. Hutson ◽  
G. Shaw ◽  
W.S. O ◽  
R.V. Short ◽  
M.B. Renfree
Keyword(s):  
Tammar Wallaby ◽  
Testicular Descent ◽  
Future Studies ◽  
Macropus Eugenii ◽  
Müllerian Inhibiting Substance ◽  
Sequence Of Events ◽  
Testicular Differentiation ◽  
Testicular Migration ◽  
Australian Marsupial ◽  
Mullerian Inhibiting Substance

The ontogeny of Mullerian inhibiting substance (MIS) production by the developing testis of an Australian marsupial, the tammar wallaby (Macropus eugenii), was determined during pouch life using an organ-culture bioassay of mouse fetal urogenital ridge. This information was related to the morphological events during testicular migration and descent. MIS biological activity was found in testes (but not ovaries or liver) of pouch young from 2 to 85 days of age. MIS production had commenced by day 2, which is within a day of the first gross morphological signs of testicular differentiation. Mullerian duct regression occurred between 10 and 30 days, which partly coincided with testicular migration to the inguinal region and enlargement of the gubernacular bulb (15 to 30 days). These observations are consistent with the hypothesis that MIS may be involved in testicular transabdominal migration. The epididymis commenced development and growth only after the testis had descended through the inguinal ring. This provides no support for the suggestion that the epididymis is involved in testicular descent into the scrotum. The basic sequence of events in post-testicular sexual differentiation in the wallaby is sufficiently similar to that seen in eutherian mammals to make it an excellent experimental model for future studies of testicular differentiation, migration and descent.

Construction of a marsupial bacterial artificial chromosome library from the model Australian marsupial, the tammar wallaby (Macropus eugenii)

2005 ◽  
Vol 53 (6) ◽  
pp. 389 ◽  
Author(s):  
Natasha Sankovic ◽  
Wayne Bawden ◽  
John Martyn ◽  
Jennifer A. M. Graves ◽  
Kurt Zuelke
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Positional Cloning ◽  
Bac Library ◽  
Artificial Chromosome ◽  
Single Copy ◽  
Tammar Wallaby ◽  
Average Insert Size ◽  
Insert Size ◽  
Macropus Eugenii ◽  
Australian Marsupial

With the accelerating recognition of the power of comparative genomics, there is now enormous interest in sequencing the genomes of a broad range of species. Marsupials diverged at an important evolutionary time. The model Australian marsupial, the tammar wallaby (Macropus eugenii), has long been a resource for biological and genetic studies of marsupials, and the availability of a bacterial artificial chromosome (BAC) library will be a valuable resource in these studies. A tammar wallaby BAC library was constructed using pRazorBAC vector. It contains 55 296 clones with an average insert size of 108 kb, representing 2.2 times coverage of the wallaby genome (based on an estimated 2.7 × 109 bp haploid genome size). The library was arrayed in 384-well plates, and spotted in duplicate onto nylon membranes. Screening these membranes has yielded clones containing 34 single-copy genes distributed over the genome, while it failed for only one gene. Each probe isolated 1–12 BAC clones and, to date, no chimeric clones have been found. This BAC library will constitute an invaluable resource for creating physical maps, positional cloning of genes and other sequences in the tammar wallaby, as well as comparative mapping studies in mammals.

Marsupial milk: a fluid source of nutrition and immune factors for the developing pouch young

2019 ◽  
Vol 31 (7) ◽  
pp. 1252 ◽  
Author(s):  
Elizabeth A. Pharo
Keyword(s):  
Reproductive Strategy ◽  
Tammar Wallaby ◽  
Milk Composition ◽  
Phase 3 ◽  
Fluid Source ◽  
Macropus Eugenii ◽  
Milk Components ◽  
Immunological Factors ◽  
Lactation Cycle ◽  
Australian Marsupial

Marsupials have a very different reproductive strategy to eutherians. An Australian marsupial, the tammar wallaby (Macropus eugenii) has a very short pregnancy of about 26.5 days, with a comparatively long lactation of 300–350 days. The tammar mother gives birth to an altricial, approximately 400 mg young that spends the first 200 days postpartum (p.p.) in its mother’s pouch, permanently (0–100 days p.p.; Phase 2A) and then intermittently (100–200 days p.p.; Phase 2B) attached to the teat. The beginning of Phase 3 marks the first exit from the pouch (akin to the birth of a precocious eutherian neonate) and the supplementation of milk with herbage. The marsupial mother progressively alters milk composition (proteins, fats and carbohydrates) and individual milk constituents throughout the lactation cycle to provide nutrients and immunological factors that are appropriate for the considerable physiological development and growth of her pouch young. This review explores the changes in tammar milk components that occur during the lactation cycle in conjunction with the development of the young.

MACROPODID MARSUPIAL LUTEINIZING HORMONE: VALIDATION OF ASSAY PROCEDURES AND CHANGES IN CONCENTRATIONS IN PLASMA DURING THE OESTROUS CYCLE IN THE FEMALE TAMMAR WALLABY (MACROPUS EUGENII)

1980 ◽  
Vol 86 (1) ◽  
pp. 1-12 ◽  
Author(s):  
R. L. SUTHERLAND ◽  
SUSAN M. EVANS ◽  
C. H. TYNDALE-BISCOE
Keyword(s):  
Limit Of Detection ◽  
Tammar Wallaby ◽  
Response Curves ◽  
Macropus Eugenii ◽  
Wide Range ◽  
Marsupial Species ◽  
Lh Rh ◽  
Lh Releasing Hormone ◽  
Australian Marsupial ◽  
Serial Dilutions

A heterologous double antibody radioimmunoassay employing a rabbit anti-ovine LH antiserum (GDN no. 15) has been developed for the assessment of concentrations of LH in macropodid marsupial pituitary extracts and plasma. In this radioimmunoassay system highly purified ovine, rat, human and kangaroo LH preparations demonstrated apparently parallel dose–response curves, as did serial dilutions of crude pituitary extracts from a wide range of Australian marsupial species and serial dilutions of plasma from ovariectomized, oestrous and LH releasing hormone (LH-RH)-treated marsupials. The assay has been used to monitor changes in immunoreactive LH in the plasma of the female tammar wallaby. Basal concentrations of LH in non-oestrous female wallabies were in the range < 0·20–1·90 ng/ml with many animals having values at or near the limit of detection of the assay. Concentrations of LH were markedly increased following ovariectomy (1·7–7·0 ng/ml), on the day of oestrus (10·0–> 50 ng/ml) and following administration of LH-RH (9·5–> 25·0 ng/ml). Plasma from hypophysectomized animals had no detectable LH immunoactivity, A well-defined LH surge, lasting approximately 12 h, was associated with oestrus. Mating occurred approximately 8 h before, and ovulation approximately 24 h after, the maximal concentration of LH in plasma.

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