Biological and biochemical characterization of two new PLA2 isoforms Cdc-9 and Cdc-10 from Crotalus durissus cumanensis snake venom

2010 ◽  
Vol 151 (1) ◽  
pp. 66-74 ◽  
Author(s):  
Frey Francisco Romero-Vargas ◽  
Luis Alberto Ponce-Soto ◽  
Daniel Martins-de-Souza ◽  
Sergio Marangoni
Keyword(s):  
Snake Venom ◽  
Biochemical Characterization ◽  
Crotalus Durissus ◽  
Crotalus Durissus Cumanensis

Biochemical and biological characterization of two serine proteinases from Colombian Crotalus durissus cumanensis snake venom

Toxicon ◽  
2013 ◽  
Vol 63 ◽  
pp. 32-43 ◽  
Author(s):  
Arley Camilo Patiño ◽  
Jaime Andrés Pereañez ◽  
José María Gutiérrez ◽  
Alexandra Rucavado
Keyword(s):  
Snake Venom ◽  
Serine Proteinases ◽  
Biological Characterization ◽  
Crotalus Durissus ◽  
Crotalus Durissus Cumanensis

Biochemical, Pharmacological and Structural Characterization of Two PLA2 Isoforms Cdr-12 and Cdr-13 from Crotalus durissus ruruima Snake Venom

2007 ◽  
Vol 26 (1) ◽  
pp. 39-49 ◽  
Author(s):  
Luis Alberto Ponce-Soto ◽  
Paulo Aparecido Baldasso ◽  
Frey Francisco Romero-Vargas ◽  
Flávia V. Winck ◽  
José Camillo Novello ◽  
...  
Keyword(s):  
Snake Venom ◽  
Structural Characterization ◽  
Crotalus Durissus

Structural and Biological Characterization of Two Crotamine Isoforms IV-2 and IV-3 Isolated from the Crotalus durissus cumanensis Venom

2007 ◽  
Vol 26 (8) ◽  
pp. 533-540 ◽  
Author(s):  
Luis Alberto Ponce-Soto ◽  
Daniel Martins ◽  
José Camillo Novello ◽  
Sergio Marangoni
Keyword(s):  
Biological Characterization ◽  
Crotalus Durissus ◽  
Crotalus Durissus Cumanensis

scholarly journals Biochemical, Pharmacological, and Structural Characterization of New Basic Bbil-TX fromBothriopsis bilineataSnake Venom

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Victor Corasolla Carregari ◽  
Rafael Stuani Floriano ◽  
Lea Rodrigues-Simioni ◽  
Flavia V. Winck ◽  
Paulo Aparecido Baldasso ◽  
...  
Keyword(s):  
Snake Venom ◽  
Protein Sequence ◽  
Maximal Activity ◽  
Optimal Conditions ◽  
High Identity ◽  
Phospholipid Hydrolysis ◽  
Rp Hplc ◽  
Crotalus Durissus ◽  
Didelphis Albiventris

Bbil-TX, a PLA2, was purified fromBothriopsis bilineatasnake venom after only one chromatographic step using RP-HPLC onμ-Bondapak C-18 column. A molecular mass of 14243.8 Da was confirmed by Q-Tof Ultima API ESI/MS (TOF MS mode) mass spectrometry. The partial protein sequence obtained was then submitted to BLASTp, with the search restricted to PLA2from snakes and shows high identity values when compared to other PLA2s. PLA2activity was presented in the presence of a synthetic substrate and showed a minimum sigmoidal behavior, reaching its maximal activity at pH 8.0 and 25–37∘C. Maximum PLA2activity required Ca2+and in the presence of Cd2+, Zn2+, Mn2+, and Mg2+it was reduced in the presence or absence of Ca2+. Crotapotin fromCrotalus durissus cascavellarattlesnake venom and antihemorrhagic factor DA2-II fromDidelphis albiventrisopossum sera under optimal conditions significantly inhibit the enzymatic activity. Bbil-TX induces myonecrosis in mice. The fraction does not show a significant cytotoxic activity in myotubes and myoblasts (C2C12). The inflammatory events induced in the serum of mice by Bbil-TX isolated fromBothriopsis bilineatasnake venom were investigated. An increase in vascular permeability and in the levels of TNF-a, IL-6, and IL-1 was was induced. Since Bbil-TX exerts a stronger proinflammatory effect, the phospholipid hydrolysis may be relevant for these phenomena.

Biochemical characterization of fibrinogenolytic serine proteinases from Vipera lebetina snake venom

Toxicon ◽  
2002 ◽  
Vol 40 (1) ◽  
pp. 51-54 ◽  
Author(s):  
Mari Samel ◽  
Juhan Subbi ◽  
Jüri Siigur ◽  
Ene Siigur
Keyword(s):  
Snake Venom ◽  
Biochemical Characterization ◽  
Serine Proteinases ◽  
Vipera Lebetina
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