Oxygen consumption, ventilation frequency and cytochrome c oxidase activity in blue cod (Parapercis colias) exposed to hydrogen sulphide or isoeugenol

2010 ◽  
Vol 151 (1) ◽  
pp. 57-65 ◽  
Author(s):  
Leonard G. Forgan ◽  
Malcolm E. Forster
Keyword(s):  
Oxygen Consumption ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Hydrogen Sulphide ◽  
Oxidase Activity ◽  
Ventilation Frequency

scholarly journals Mechanical Ventilation Preserves Diaphragm Mitochondrial Function in a Rat Sepsis Model

2020 ◽  
Author(s):  
Pierre Eyenga ◽  
Damien Roussel ◽  
Benjamin Rey ◽  
Patrice Ndille ◽  
Loic Teulier ◽  
...  
Keyword(s):  
Mechanical Ventilation ◽  
Oxygen Consumption ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Oxidase Activity ◽  
Ros Generation ◽  
Mitochondrial Oxygen Consumption ◽  
Atp Production ◽  
Mitochondrial Ros ◽  
Mda Content

Abstract Background: To describe the effect of mechanical ventilation on diaphragm mitochondrial oxygen consumption, ATP production, reactive oxygen species (ROS) generation, and cytochrome-c oxidase activity and content, and their relationship to diaphragm strength in an experimental model of sepsis.Methods: A cecal ligation and puncture (CLP) protocol was performed in 12 rats while 12 controls underwent sham-operation. Half of the rats in each group were paralyzed and mechanically ventilated. We performed blood gas analysis and lactic acid assays 6 hours after surgery. Afterwards, we measured diaphragm strength and mitochondrial oxygen consumption, ATP and ROS generation, and cytochrome-c oxidase activity. We also measured malondialdehyde (MDA) content as an index of lipid peroxidation, and mRNA expression of the pro-inflammatory interleukin-1β (IL-1β) in diaphragms.Results: CLP rats showed severe hypotension, metabolic acidosis, and upregulation of diaphragm IL-1β mRNA expression. Compared to sham controls, spontaneously breathing CLP rats showed lower diaphragm force and increased susceptibility to fatigue, along with depressed mitochondrial oxygen consumption and ATP production and cytochrome-c oxidase activity. These rats also showed increased mitochondrial ROS generation and MDA content. Mechanical ventilation markedly restored mitochondrial oxygen consumption and ATP production in CLP rats; lowered mitochondrial ROS production by the complex 3; and preserved cytochrome-c oxidase activity.Conclusion: In an experimental model of sepsis, early initiation of mechanical ventilation restores diaphragm mitochondrial function.

Cytochrome aa3 in facultatively aerobic and hyperthermophilic archaeon Pyrobaculum oguniense

2005 ◽  
Vol 51 (8) ◽  
pp. 621-627 ◽  
Author(s):  
Takuro Nunoura ◽  
Yoshihiko Sako ◽  
Takayoshi Wakagi ◽  
Aritsune Uchida
Keyword(s):  
Oxygen Consumption ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Gene Product ◽  
Respiratory Chain ◽  
Oxidase Activity ◽  
Terminal Oxidase ◽  
Hyperthermophilic Archaeon ◽  
Consumption Activity ◽  
Copper Oxidase

We partially purified and characterized the cytochrome aa3 from the facultatively aerobic and hyperthermophilic archaeon Pyrobaculum oguniense. This cytochrome aa3 showed oxygen consumption activity with N, N, N′, N′-tetramethyl-1,4-phenylenediamine and ascorbate as substrates, and also displayed bovine cytochrome c oxidase activity. These enzymatic activities of cytochrome aa3 were inhibited by cyanide and azide. This cytochrome contained heme As, but not typical heme A. An analysis of trypsin-digested fragments indicated that 1 subunit of this cytochrome was identical to the gene product of subunit I of the SoxM-type heme – copper oxidase (poxC). This is the first report of a terminal oxidase in hyperthermophilic crenarchaeon belonging to the order Thermoproteales.Key words: aerobic respiratory chain, terminal oxidase, Archaea, hyperthermophile, Pyrobaculum.

scholarly journals Mechanical ventilation preserves diaphragm mitochondrial function in a rat sepsis model

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
P. Eyenga ◽  
D. Roussel ◽  
B. Rey ◽  
P. Ndille ◽  
L. Teulier ◽  
...  
Keyword(s):  
Mechanical Ventilation ◽  
Oxygen Consumption ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Oxidase Activity ◽  
Ros Generation ◽  
Mitochondrial Oxygen Consumption ◽  
Atp Production ◽  
Mitochondrial Ros ◽  
Mda Content

Abstract Background To describe the effect of mechanical ventilation on diaphragm mitochondrial oxygen consumption, ATP production, reactive oxygen species (ROS) generation, and cytochrome c oxidase activity and content, and their relationship to diaphragm strength in an experimental model of sepsis. Methods A cecal ligation and puncture (CLP) protocol was performed in 12 rats while 12 controls underwent sham operation. Half of the rats in each group were paralyzed and mechanically ventilated. We performed blood gas analysis and lactic acid assays 6 h after surgery. Afterwards, we measured diaphragm strength and mitochondrial oxygen consumption, ATP and ROS generation, and cytochrome c oxidase activity. We also measured malondialdehyde (MDA) content as an index of lipid peroxidation, and mRNA expression of the proinflammatory interleukin-1β (IL-1β) in diaphragms. Results CLP rats showed severe hypotension, metabolic acidosis, and upregulation of diaphragm IL-1β mRNA expression. Compared to sham controls, spontaneously breathing CLP rats showed lower diaphragm force and increased susceptibility to fatigue, along with depressed mitochondrial oxygen consumption and ATP production and cytochrome c oxidase activity. These rats also showed increased mitochondrial ROS generation and MDA content. Mechanical ventilation markedly restored mitochondrial oxygen consumption and ATP production in CLP rats; lowered mitochondrial ROS production by the complex 3; and preserved cytochrome c oxidase activity. Conclusion In an experimental model of sepsis, early initiation of mechanical ventilation restores diaphragm mitochondrial function.

scholarly journals A genome-wide copper-sensitized screen identifies novel regulators of mitochondrial cytochrome c oxidase activity

2021 ◽  
Vol 296 ◽  
pp. 100485
Author(s):  
Natalie M. Garza ◽  
Aaron T. Griffin ◽  
Mohammad Zulkifli ◽  
Chenxi Qiu ◽  
Craig D. Kaplan ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Oxidase Activity ◽  
Mitochondrial Cytochrome ◽  
Genome Wide ◽  
A Genome ◽  
Mitochondrial Cytochrome C

Effect of subunit III removal on control of cytochrome c oxidase activity by pH

Biochemistry ◽  
1988 ◽  
Vol 27 (17) ◽  
pp. 6307-6314 ◽  
Author(s):  
Linda C. Gregory ◽  
Shelagh Ferguson-Miller
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Oxidase Activity ◽  
Subunit Iii

A novel a-type terminal oxidase from Sulfolobusacidocaldarius with cytochrome c oxidase activity

1989 ◽  
Vol 165 (3) ◽  
pp. 1110-1114 ◽  
Author(s):  
Takayoshi Wakagi ◽  
Tatsuo Yamauchi ◽  
Tairo Oshima ◽  
Michele Müller ◽  
Angello Azzi ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Oxidase Activity ◽  
Terminal Oxidase

Metarhizin A suppresses cell proliferation by inhibiting cytochrome c oxidase activity

Life Sciences ◽  
2014 ◽  
Vol 103 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Yasuhiro Katou ◽  
Naoya Endo ◽  
Toshiyuki Suzuki ◽  
Jiang Yu ◽  
Haruhisa Kikuchi ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Oxidase Activity

Changes in the Cytochrome c Oxidase Activity in Response to Light Regime for Photosynthesis Observed with the Cyanophyte Synechocystis PCC 6714

1994 ◽  
Vol 35 (8) ◽  
pp. 1135-1140 ◽  
Author(s):  
Tian-Qing Gu ◽  
Yumi Iwama ◽  
Akio Murakami ◽  
Siba Prasad Adhikary ◽  
Yoshihiko Fujita
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Oxidase Activity ◽  
Light Regime ◽  
Synechocystis Pcc 6714

Interaction of aging and intermittent ethanol exposure on brain cytochrome c oxidase activity levels

Alcohol ◽  
2003 ◽  
Vol 29 (2) ◽  
pp. 91-100 ◽  
Author(s):  
Pia Jaatinen ◽  
Jarno Riikonen ◽  
Päivi Riihioja ◽  
Olli Kajander ◽  
Antti Hervonen
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Oxidase Activity ◽  
Ethanol Exposure ◽  
Activity Levels
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