scholarly journals The long amplicon quantitative PCR for DNA damage assay as a sensitive method of assessing DNA damage in the environmental model, Atlantic killifish (Fundulus heteroclitus)

2009 ◽  
Vol 149 (2) ◽  
pp. 182-186 ◽  
Author(s):  
Dawoon Jung ◽  
Youngeun Cho ◽  
Joel N. Meyer ◽  
Richard T. Di Giulio
Keyword(s):  
Dna Damage ◽  
Quantitative Pcr ◽  
Fundulus Heteroclitus ◽  
Environmental Model ◽  
Sensitive Method ◽  
Atlantic Killifish

scholarly journals Effects of benzo[a]pyrene on mitochondrial and nuclear DNA damage in Atlantic killifish (Fundulus heteroclitus) from a creosote-contaminated and reference site

2009 ◽  
Vol 95 (1) ◽  
pp. 44-51 ◽  
Author(s):  
Dawoon Jung ◽  
Youngeun Cho ◽  
Leonard B. Collins ◽  
James A. Swenberg ◽  
Richard T. Di Giulio
Keyword(s):  
Dna Damage ◽  
Fundulus Heteroclitus ◽  
Reference Site ◽  
Nuclear Dna ◽  
Atlantic Killifish

scholarly journals Profiling microRNA expression in Atlantic killifish (Fundulus heteroclitus) gill and responses to arsenic and hyperosmotic stress

2019 ◽  
Vol 206 ◽  
pp. 142-153 ◽  
Author(s):  
Britton C. Goodale ◽  
Thomas H. Hampton ◽  
Emily N. Ford ◽  
Craig E. Jackson ◽  
Joseph R. Shaw ◽  
...  
Keyword(s):  
Fundulus Heteroclitus ◽  
Microrna Expression ◽  
Hyperosmotic Stress ◽  
Atlantic Killifish

Quantitative PCR for detection of DNA damage in mitochondrial DNA of the fission yeast Schizosaccharomyces pombe

2016 ◽  
Vol 127 ◽  
pp. 77-81 ◽  
Author(s):  
Takanori Senoo ◽  
Mayumi Yamanaka ◽  
Atori Nakamura ◽  
Tomoki Terashita ◽  
Shinji Kawano ◽  
...  
Keyword(s):  
Dna Damage ◽  
Mitochondrial Dna ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Quantitative Pcr

Salinity-dependent silver nanoparticle uptake and transformation by Atlantic killifish (Fundulus heteroclitus) embryos

2013 ◽  
Vol 8 (sup1) ◽  
pp. 167-176 ◽  
Author(s):  
Melanie Auffan ◽  
Cole W. Matson ◽  
Jerome Rose ◽  
Mariah Arnold ◽  
Olivier Proux ◽  
...  
Keyword(s):  
Silver Nanoparticle ◽  
Fundulus Heteroclitus ◽  
Nanoparticle Uptake ◽  
Atlantic Killifish

scholarly journals Adhesion of Enterococcus faecalis in the Nonculturable State to Plankton Is the Main Mechanism Responsible for Persistence of This Bacterium in both Lake and Seawater

2004 ◽  
Vol 70 (11) ◽  
pp. 6892-6896 ◽  
Author(s):  
Caterina Signoretto ◽  
Gloria Burlacchini ◽  
Maria del Mar Lle� ◽  
Carla Pruzzo ◽  
Massimiliano Zampini ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Enterococcus Faecalis ◽  
Lake Water ◽  
Adriatic Sea ◽  
Fecal Indicators ◽  
Pcr Assay ◽  
Culture Methods ◽  
Lake Garda ◽  
Nonculturable State ◽  
Sensitive Method

ABSTRACT The presence of enterococci in lake and seawater in an 18-month survey comparing molecular (PCR and quantitative PCR) and culture methods was evaluated, as well as the possibility that zooplankton could act as reservoirs for enterococci. Samples of both water and zooplankton were collected monthly from a Lake Garda site and an Adriatic Sea site. In lake water, the positive samples numbered 13 of 54 (24%) by culture and 32 of 54 (59%) when PCR was applied. In seawater, they numbered 0 of 51 by culture and 18 of 51 (35%) by PCR. Enterococci were found either totally bound to plankton or totally in water, depending on the presence or absence of plankton, respectively. These results clearly indicate that the PCR assay is a powerful tool for detecting fecal indicators and pathogens in the environment, thus providing a much more sensitive method than culture.

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