ACL-I, a lectin from the marine sponge Axinella corrugata: Isolation, characterization and chemotactic activity

2008 ◽  
Vol 148 (1) ◽  
pp. 23-30 ◽  
Author(s):  
Roger R. Dresch ◽  
Gilberto D. Zanetti ◽  
Cléa B. Lerner ◽  
Beatriz Mothes ◽  
Vera M.T. Trindade ◽  
...  
Keyword(s):  
Marine Sponge ◽  
Chemotactic Activity ◽  
Axinella Corrugata

Staining tumor cells with biotinylated ACL-I, a lectin isolated from the marine sponge, Axinella corrugata

2012 ◽  
Vol 88 (1) ◽  
pp. 1-9 ◽  
Author(s):  
RR Dresch ◽  
GD Zanetti ◽  
FJ Irazoqui ◽  
VG Sendra ◽  
N Zlocowski ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Marine Sponge ◽  
Axinella Corrugata

Immunohistochemical localization of an N-acetyl amino-carbohydrate specific lectin (ACL-I) of the marine sponge Axinella corrugata

2011 ◽  
Vol 113 (6) ◽  
pp. 671-674 ◽  
Author(s):  
Roger Remy Dresch ◽  
Gilberto Dolejal Zanetti ◽  
João Henrique Corrêa Kanan ◽  
Beatriz Mothes ◽  
Cléa Beatriz Lerner ◽  
...  
Keyword(s):  
Marine Sponge ◽  
Immunohistochemical Localization ◽  
Axinella Corrugata

Development of a red-shifted fluorescence-based assay for SARS-coronavirus 3CL protease: identification of a novel class of anti-SARS agents from the tropical marine sponge Axinella corrugata

2006 ◽  
Vol 387 (8) ◽  
pp. 1063-1074 ◽  
Author(s):  
Pamela Hamill ◽  
Derek Hudson ◽  
Richard Y. Kao ◽  
Polly Chow ◽  
Meera Raj ◽  
...  
Keyword(s):  
Marine Sponge ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Acid Ethyl Ester ◽  
Coumarin Derivative ◽  
Sars Coronavirus ◽  
Acceptor Pair ◽  
Donor And Acceptor ◽  
Axinella Corrugata ◽  
Black Hole Quencher

Abstract SARS-coronavirus (SARS-CoV) encodes a main protease, 3CLpro, which plays an essential role in the viral life cycle and is currently the prime target for discovering new anti-coronavirus agents. In this article, we report our success in developing a novel red-shifted (RS) fluorescence-based assay for 3CLpro and its application for identifying small-molecule anti-SARS agents from marine organisms. We have synthesised and characterised the first generation of a red-shifted internally quenched fluorogenic substrate (RS-IQFS) for 3CLpro based on resonance energy transfer between the donor and acceptor pair CAL Fluor Red 610 and Black Hole Quencher-1 (K m and k cat values of 14 μM and 0.65 min-1). The RS-IQFS primary sequence was selected based on the results of our screening analysis of 3CLpro performed using a series of blue-shifted (BS)-IQFSs corresponding to the 3CLpro-mediated cleavage junctions of the SARS-CoV polyproteins. In contrast to BS-IQFSs, the RS-IQFS was not susceptible to fluorescence interference from coloured samples and allowed for successful screening of marine natural products and identification of a coumarin derivative, esculetin-4-carboxylic acid ethyl ester, a novel 3CLpro inhibitor (IC50=46 μM) and anti-SARS agent (EC50=112 μM; median toxic concentration >800 μM) from the tropical marine sponge Axinella corrugata.

Corrugoside, a new immunostimulatory α-galactoglycosphingolipid from the marine sponge Axinella corrugata☆

2008 ◽  
Vol 16 (4) ◽  
pp. 2077-2085 ◽  
Author(s):  
Valeria Costantino ◽  
Ernesto Fattorusso ◽  
Concetta Imperatore ◽  
Alfonso Mangoni ◽  
Stefan Freigang ◽  
...  
Keyword(s):  
Marine Sponge ◽  
Axinella Corrugata

Scopularides A and B, two new cyclodepsipeptides from the marine sponge-derived fungus Scopulariopsis brevicaulis

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
I Kajahn ◽  
Z Yu ◽  
G Lang ◽  
R Schmaljohann ◽  
JF Imhoff
Keyword(s):  
Marine Sponge ◽  
Scopulariopsis Brevicaulis

New cystine knot peptides from a marine sponge Asteropus sp.

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
J Jung ◽  
H Li ◽  
J Li ◽  
C Lee ◽  
W Lee ◽  
...  
Keyword(s):  
Marine Sponge ◽  
Cystine Knot

Pentacyclic ingamine-type alkaloids, a new antiplasmodial pharmacophore from the marine sponge Petrosid Ng5 Sp5

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
I Muhammad ◽  
MA Ibrahim ◽  
S Khan ◽  
M Jacob ◽  
BL Tekwani ◽  
...  
Keyword(s):  
Marine Sponge
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