Host regulation and release of parasitism-specific proteins in the system Toxoneuron nigriceps–Heliothis virescens

Author(s):  
F.L. Cônsoli ◽  
S.L. Brandt ◽  
T.A. Coudron ◽  
S.B. Vinson
2003 ◽  
Vol 49 (11) ◽  
pp. 1021-1030 ◽  
Author(s):  
Sheng Li ◽  
Patrizia Falabella ◽  
Indira Kuriachan ◽  
S.Bradleigh Vinson ◽  
David W Borst ◽  
...  

2008 ◽  
Vol 332 (2) ◽  
pp. 371-379 ◽  
Author(s):  
Gianluca Tettamanti ◽  
Annalisa Grimaldi ◽  
Francesco Pennacchio ◽  
Magda de Eguileor

2006 ◽  
Vol 41 (4) ◽  
pp. 321-328 ◽  
Author(s):  
P. Glynn Tillman

Host searching behavior of females of the endoparasitoid Toxoneuron nigriceps Vierick (Hymenoptera: Braconidae) was observed in the field for three tobacco herbivores, Heliothis virescens (F.), Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae), and Manduca sexta L. (Lepidoptera: Sphingidae). The only developmentally suitable host for the parasitoid was H. virescens. Hovering, searching, and oviposition (for plants with herbivores) by T. nigriceps were observed for a combination of two plant types, plants with only herbivore-induced plant volatiles or damaged plants with herbivores, and three insect species. The six treatments were as follows: (1) plants with only H. virescens-induced plant volatiles, (2) plants with only H. zea-induced plant volatiles, (3) plants with only M. sexta-induced plant volatiles, (4) plants damaged by H. virescens, (5) plants damaged by H. zea, and (6) plants damaged by M. sexta. Parasitoid females readily hovered around and searched on plants of both types. However, females spent more time foraging for H. virescens than for the nonhosts, H. zea and M. sexta. For plants with only herbivore-induced plant volatiles, T. nigriceps females spent more time searching on H. virescens plants than on H. zea and M. sexta plants. For damaged plants, parasitoid females visited more H. virescens plants than M. sexta plants and spent more time searching for H. virescens larvae than for M. sexta and H. zea larvae. Even though T. nigriceps females laid eggs in M. sexta and H. zea larvae, oviposition was higher for H. virescens than for the nonhosts. In conclusion, T. nigriceps females utilized more of their energy searching for and parasitizing H. virescens over the nonhosts on tobacco.


2011 ◽  
Vol 11 (3) ◽  
pp. 1-11 ◽  
Author(s):  
Indira Kuriachan ◽  
Ruth Henderson ◽  
Rachel Laca ◽  
S. Bradleigh Vinson

Author(s):  
L. M. Marshall

A human erythroleukemic cell line, metabolically blocked in a late stage of erythropoiesis, becomes capable of differentiation along the normal pathway when grown in the presence of hemin. This process is characterized by hemoglobin synthesis followed by rearrangement of the plasma membrane proteins and culminates in asymmetrical cytokinesis in the absence of nuclear division. A reticulocyte-like cell buds from the nucleus-containing parent cell after erythrocyte specific membrane proteins have been sequestered into its membrane. In this process the parent cell faces two obstacles. First, to organize its erythrocyte specific proteins at one pole of the cell for inclusion in the reticulocyte; second, to reduce or abolish membrane protein turnover since hemoglobin is virtually the only protein being synthesized at this stage. A means of achieving redistribution and cessation of turnover could involve movement of membrane proteins by a directional lipid flow. Generation of a lipid flow towards one pole and accumulation of erythrocyte-specific membrane proteins could be achieved by clathrin coated pits which are implicated in membrane endocytosis, intracellular transport and turnover. In non-differentiating cells, membrane proteins are turned over and are random in surface distribution. If, however, the erythrocyte specific proteins in differentiating cells were excluded from endocytosing coated pits, not only would their turnover cease, but they would also tend to drift towards and collect at the site of endocytosis. This hypothesis requires that different protein species are endocytosed by the coated vesicles in non-differentiating than by differentiating cells.


Author(s):  
W.A. Carrington ◽  
F.S. Fay ◽  
K.E. Fogarty ◽  
L. Lifshitz

Advances in digital imaging microscopy and in the synthesis of fluorescent dyes allow the determination of 3D distribution of specific proteins, ions, GNA or DNA in single living cells. Effective use of this technology requires a combination of optical and computer hardware and software for image restoration, feature extraction and computer graphics.The digital imaging microscope consists of a conventional epifluorescence microscope with computer controlled focus, excitation and emission wavelength and duration of excitation. Images are recorded with a cooled (-80°C) CCD. 3D images are obtained as a series of optical sections at .25 - .5 μm intervals.A conventional microscope has substantial blurring along its optical axis. Out of focus contributions to a single optical section cause low contrast and flare; details are poorly resolved along the optical axis. We have developed new computer algorithms for reversing these distortions. These image restoration techniques and scanning confocal microscopes yield significantly better images; the results from the two are comparable.


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