Cloning and expression of a pivotal calcium metabolism regulator: calmodulin involved in shell formation from pearl oyster (Pinctada fucata)

2004 ◽  
Vol 138 (3) ◽  
pp. 235-243 ◽  
Author(s):  
Shuo Li ◽  
Liping Xie ◽  
Cen Zhang ◽  
Yong Zhang ◽  
Mianzhi Gu ◽  
...  
Keyword(s):  
Calcium Metabolism ◽  
Pearl Oyster ◽  
Pinctada Fucata ◽  
Cloning And Expression ◽  
Shell Formation

Molecular Cloning and Expression of a Pearl Oyster ( Pinctada fucata ) Homologue of Mammalian Putative Tumor Suppressor QM

2004 ◽  
Vol 6 (1) ◽  
pp. 8-16 ◽  
Author(s):  
Yong Zhang ◽  
Jie Huang ◽  
Qingxiong Meng ◽  
Tiemin Jiang ◽  
Liping Xie ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Molecular Cloning ◽  
Pearl Oyster ◽  
Pinctada Fucata ◽  
Cloning And Expression ◽  
Putative Tumor Suppressor

scholarly journals Molecular Cloning and Characterization of Full-Length cDNA of Calmodulin Gene from Pacific OysterCrassostrea gigas

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Xing-Xia Li ◽  
Wen-Chao Yu ◽  
Zhong-Qiang Cai ◽  
Cheng He ◽  
Na Wei ◽  
...  
Keyword(s):  
Calcium Binding ◽  
Pacific Oyster ◽  
Pearl Oyster ◽  
Full Length ◽  
Pinctada Fucata ◽  
Whole Genome Sequence ◽  
Formation Mechanisms ◽  
Shell Formation ◽  
Full Length Cdna ◽  
Ef Hand

The shell of the pearl oyster (Pinctada fucata) mainly comprises aragonite whereas that of the Pacific oyster (Crassostrea gigas) is mainly calcite, thereby suggesting the different mechanisms of shell formation between above two mollusks. Calmodulin (CaM) is an important gene for regulating the uptake, transport, and secretion of calcium during the process of shell formation in pearl oyster. It is interesting to characterize the CaM in oysters, which could facilitate the understanding of the different shell formation mechanisms among mollusks. We cloned the full-length cDNA of Pacific oyster CaM (cgCaM) and found that the cgCaM ORF encoded a peptide of 113 amino acids containing three EF-hand calcium-binding domains, its expression level was highest in the mantle, hinting that the cgCaM gene is probably involved in shell formation of Pacific oyster, and the common ancestor of Gastropoda and Bivalvia may possess at least three CaM genes. We also found that the numbers of some EF hand family members in highly calcified species were higher than those in lowly calcified species and the numbers of these motifs in oyster genome were the highest among the mollusk species with whole genome sequence, further hinting the correlation between CaM and biomineralization.

scholarly journals Cloning and expression pattern of a Smad3 homolog from the pearl oyster, Pinctada fucata

2008 ◽  
Vol 40 (3) ◽  
pp. 244-252 ◽  
Author(s):  
Yujuan Zhou ◽  
Zuoxin He ◽  
Qing Li ◽  
Liping Xie ◽  
Rongqing Zhang
Keyword(s):  
Expression Pattern ◽  
Pearl Oyster ◽  
Pinctada Fucata ◽  
Cloning And Expression

scholarly journals Transcriptional regulation of the matrix protein Shematrin-2 during shell formation in pearl oyster

2018 ◽  
Vol 293 (46) ◽  
pp. 17803-17816 ◽  
Author(s):  
Yan Chen ◽  
Jing Gao ◽  
Jun Xie ◽  
Jian Liang ◽  
Guilan Zheng ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Transcription Factors ◽  
Calcium Carbonate ◽  
Matrix Protein ◽  
Pearl Oyster ◽  
Pinctada Fucata ◽  
Functional Assay ◽  
Shell Formation ◽  
Molluscan Shell ◽  
The Matrix

The molluscan shell is a fascinating biomineral consisting of a highly organized calcium carbonate composite. Biomineralization is elaborately controlled and involves several macromolecules, especially matrix proteins, but little is known about the regulatory mechanisms. The matrix protein Shematrin-2, expression of which peaks in the mantle tissues and in the shell components of the pearl oyster Pinctada fucata, has been suggested to be a key participant in biomineralization. Here, we expressed and purified Shematrin-2 from P. fucata and explored its function and transcriptional regulation. An in vitro functional assay revealed that Shematrin-2 binds the calcite, aragonite, and chitin components of the shell, decreases the rate of calcium carbonate deposition, and changes the morphology of the deposited crystal in the calcite crystallization system. Furthermore, we cloned the Shematrin-2 gene promoter, and analysis of its sequence revealed putative binding sites for the transcription factors CCAAT enhancer–binding proteins (Pf-C/EBPs) and nuclear factor-Y (NF-Y). Using transient co-transfection and reporter gene assays, we found that cloned and recombinantly expressed Pf-C/EBP-A and Pf-C/EBP-B greatly and dose-dependently up-regulate the promoter activity of the Shematrin-2 gene. Importantly, Pf-C/EBP-A and Pf-C/EBP-B knockdowns decreased Shematrin-2 gene expression and induced changes in the inner-surface structures in prismatic layers that were similar to those of antibody-based Shematrin-2 inhibition. Altogether, our data reveal that the transcription factors Pf-C/EBP-A and Pf-C/EBP-B up-regulate the expression of the matrix protein Shematrin-2 during shell formation in P. fucata, improving our understanding of the transcriptional regulation of molluscan shell development at the molecular level.

Characterization of organic matrix components of pearl oyster, Pinctada fucata and their implications in shell formation

2008 ◽  
Vol 2 (2) ◽  
pp. 156-161 ◽  
Author(s):  
Chihiro Nogawa ◽  
Mami Obara ◽  
Megumi Ozawa ◽  
Aya Sato ◽  
Akiko Watanabe ◽  
...  
Keyword(s):  
Organic Matrix ◽  
Pearl Oyster ◽  
Pinctada Fucata ◽  
Shell Formation ◽  
Matrix Components

scholarly journals fam20C participates in the shell formation in the pearl oyster, Pinctada fucata

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Jinzhe Du ◽  
Chuang Liu ◽  
Guangrui Xu ◽  
Jun Xie ◽  
Liping Xie ◽  
...  
Keyword(s):  
Pearl Oyster ◽  
Pinctada Fucata ◽  
Shell Formation
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