Characterization of two heat shock proteins (Hsp70/Hsc70) from grass carp (Ctenopharyngodon idella): Evidence for their differential gene expression, protein synthesis and secretion in LPS-challenged peripheral blood lymphocytes

2011 ◽  
Vol 159 (2) ◽  
pp. 109-114 ◽  
Author(s):  
Anying Zhang ◽  
Xiaofei Zhou ◽  
Xinyan Wang ◽  
Hong Zhou
Keyword(s):  
Gene Expression ◽  
Protein Synthesis ◽  
Heat Shock ◽  
Grass Carp ◽  
Peripheral Blood Lymphocytes ◽  
Ctenopharyngodon Idella ◽  
Grass Carp Ctenopharyngodon Idella ◽  
Differential Gene ◽  
Shock Proteins

scholarly journals Molecular Characterization of Hsp47 in Grass Carp (Ctenopharyngodon idella) and Its Correlation with Type I Collagen in Response to Fish Aerobic Exercise

Fishes ◽  
2021 ◽  
Vol 6 (2) ◽  
pp. 17
Author(s):  
Xiao Liang ◽  
Ying Wan ◽  
Zhiyuan Shen ◽  
Yanmei Liu ◽  
Dapeng Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Aerobic Exercise ◽  
Grass Carp ◽  
Type I Collagen ◽  
Collagen Content ◽  
Ctenopharyngodon Idella ◽  
Type I ◽  
Grass Carp Ctenopharyngodon Idella ◽  
Different Tissues

Heat shock protein 47 (Hsp47) is a collagen-specific molecular chaperone that is indispensable for molecular maturation of collagen. In this study, hsp47 and hsp47-like cDNAs were cloned and characterized in grass carp (Ctenopharyngodon idella). The cDNAs were 1212 and 1218 base pairs long, respectively, and included an open reading frame encoding 403 and 405 amino acids. The molecular phylogeny based on the deduced amino acid sequences indicated that the correct sequences of the hsp47 and hsp47-like cDNA were obtained and the deduced proteins clustered distinctly into teleost clades. Primary structure analysis and characterization of Hsp47 and Hsp47-like shared the basic structure and biofunctions of Hsp47 in vertebrates. The spatial pattern of gene expression revealed that hsp47 and hsp47-like were relatively ubiquitous in different tissues and highly expressed in heart and skin. The expression levels of hsp47 and hsp47-like and type I collagen mRNAs varied similarly in different tissues. Type I collagen content increased significantly with the increase of water velocity in the muscle of grass carp in response to aerobic exercise. Among the gene expression changes of hsp47, hsp47-like, col1a1 and col1a2 in muscle that occurred in response to aerobic exercise, the change of type I collagen was most strongly correlated with hsp47 expression. Additionally, col1a1 showed the highest correlation with hsp47-like and col1a2 showed the highest correlation with hsp47. These findings suggest that grass carp Hsp47 and Hsp47-like are closely related to type I collagen synthesis. This study firstly suggests fish aerobic exercise can improve type I collagen content and Hsp47 gene expression in muscle of grass carp.

Identification and molecular characterization of peroxiredoxin 2 in grass carp (Ctenopharyngodon idella)

2019 ◽  
Vol 92 ◽  
pp. 570-582
Author(s):  
Denghui Zhu ◽  
Rong Huang ◽  
Cheng Yang ◽  
Peipei Fu ◽  
Liangming Chen ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Grass Carp ◽  
Ctenopharyngodon Idella ◽  
Peroxiredoxin 2 ◽  
Grass Carp Ctenopharyngodon Idella
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