cDNA cloning and in situ hybridization of a novel lysozyme in the Pacific oyster, Crassostrea gigas

2007 ◽  
Vol 148 (2) ◽  
pp. 160-166 ◽  
Author(s):  
Naoki Itoh ◽  
Keisuke G. Takahashi
Keyword(s):  
In Situ Hybridization ◽  
Cdna Cloning ◽  
Crassostrea Gigas ◽  
Pacific Oyster ◽  
The Pacific

Observations raise the question if the Pacific oyster, Crassostrea gigas, can act as either a carrier or a reservoir for Bonamia ostreae or Bonamia exitiosa

Parasitology ◽  
2010 ◽  
Vol 137 (10) ◽  
pp. 1515-1526 ◽  
Author(s):  
S. A. LYNCH ◽  
E. ABOLLO ◽  
A. RAMILO ◽  
A. CAO ◽  
S. C. CULLOTY ◽  
...  
Keyword(s):  
Crassostrea Gigas ◽  
Pacific Oyster ◽  
Ostrea Edulis ◽  
Connective Tissues ◽  
Tissue Samples ◽  
Pcr Screening ◽  
The Pacific ◽  
Bonamia Ostreae ◽  
Polymerase Chain

SUMMARYThis study investigated the ability of the Pacific oyster, Crassostrea gigas, to act as a carrier or reservoir of the protistan Bonamia ostreae. Studies were carried out independently in Ireland and in Spain. Naïve C. gigas were exposed to B. ostreae both in the field and in the laboratory via natural exposure or experimental injection. Naïve flat oysters, Ostrea edulis, were placed in tanks with previously exposed C. gigas. Oysters were screened for B. ostreae by examination of ventricular heart smears and by polymerase chain reaction (PCR) screening of tissue samples (gill and/or heart) and shell cavity fluid. PCR-positive oysters were further screened using histology and in situ hybridization (ISH). B. ostreae DNA was detected in the tissues and/or shell cavity fluid of a small number of C. gigas in the field and in the laboratory. B. ostreae-like cells were visualized in the haemocytes of 1 C. gigas and B. ostreae-like cells were observed extracellularly in the connective tissues of 1 other C. gigas. When C. gigas naturally exposed to B. ostreae were held with naïve O. edulis, B. ostreae DNA was detected in O. edulis; however, B. ostreae cells were not visualized. In Spain, B. exitiosa DNA was also detected in Pacific oyster tissues. The results of this study have important implications for C. gigas transfers from B. ostreae-endemic areas to uninfected areas and highlight B. ostreae and B. exitiosa's ability to survive extracellularly and in other non-typical hosts.

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