Suppression of circadian locomotor rhythm by per dsRNA in the cricket Gryllus bimaculatus

2006 ◽  
Vol 145 (3-4) ◽  
pp. 408
Author(s):  
Yoshiyuki Moriyama ◽  
Kenji Tomioka
Keyword(s):  
Gryllus Bimaculatus ◽  
Locomotor Rhythm

scholarly journals Phase Shifts of the Circadian Locomotor Rhythm Induced by Pigment-Dispersing Factor in the Cricket Gryllus bimaculatus

2003 ◽  
Vol 20 (11) ◽  
pp. 1347-1354 ◽  
Author(s):  
Muniyandi Singaravel ◽  
Yuko Fujisawa ◽  
Miki Hisada ◽  
A. S. M. Saifullah ◽  
Kenji Tomioka
Keyword(s):  
Phase Shifts ◽  
Gryllus Bimaculatus ◽  
Locomotor Rhythm ◽  
Pigment Dispersing Factor

scholarly journals Enhancer of zeste plays an important role in photoperiodic modulation of locomotor rhythm in the cricket, Gryllus bimaculatus

2016 ◽  
Vol 2 (1) ◽  
Author(s):  
Yoshimasa Hamada ◽  
Atsushi Tokuoka ◽  
Tetsuya Bando ◽  
Hideyo Ohuchi ◽  
Kenji Tomioka
Keyword(s):  
Gryllus Bimaculatus ◽  
Locomotor Rhythm ◽  
Enhancer Of Zeste

scholarly journals The role of clockwork orange in the circadian clock of the cricket Gryllus bimaculatus

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Yasuaki Tomiyama ◽  
Tsugumichi Shinohara ◽  
Mirai Matsuka ◽  
Tetsuya Bando ◽  
Taro Mito ◽  
...  
Keyword(s):  
Circadian Clock ◽  
Clock Genes ◽  
Clock Cycle ◽  
Constant Darkness ◽  
Mrna Levels ◽  
Gryllus Bimaculatus ◽  
Locomotor Rhythm ◽  
Rhythmic Expression ◽  
Cryptochrome 2

Abstract The circadian clock generates rhythms of approximately 24 h through periodic expression of the clock genes. In insects, the major clock genes period (per) and timeless (tim) are rhythmically expressed upon their transactivation by CLOCK/CYCLE, with peak levels in the early night. In Drosophila, clockwork orange (cwo) is known to inhibit the transcription of per and tim during the daytime to enhance the amplitude of the rhythm, but its function in other insects is largely unknown. In this study, we investigated the role of cwo in the clock mechanism of the cricket Gryllus bimaculatus. The results of quantitative RT-PCR showed that under a light/dark (LD) cycle, cwo is rhythmically expressed in the optic lobe (lamina-medulla complex) and peaks during the night. When cwo was knocked down via RNA interference (RNAi), some crickets lost their locomotor rhythm, while others maintained a rhythm but exhibited a longer free-running period under constant darkness (DD). In cwoRNAi crickets, all clock genes except for cryptochrome 2 (cry2) showed arrhythmic expression under DD; under LD, some of the clock genes showed higher mRNA levels, and tim showed rhythmic expression with a delayed phase. Based on these results, we propose that cwo plays an important role in the cricket circadian clock.

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