Real-time PCR analysis of ovary- and brain-type aromatase gene expression during Atlantic halibut (Hippoglossus hippoglossus) development

2006 ◽  
Vol 144 (1) ◽  
pp. 128-135 ◽  
Author(s):  
Makoto P. Matsuoka ◽  
Solveig van Nes ◽  
Øivind Andersen ◽  
Tillmann J. Benfey ◽  
Michael Reith
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Analysis ◽  
Atlantic Halibut ◽  
Hippoglossus Hippoglossus ◽  
Aromatase Gene ◽  
Aromatase Gene Expression

Evaluation of reference genes for quantitative real-time PCR analysis of gene expression in Hainan medaka (Oryzias curvinotus)

Gene Reports ◽  
2019 ◽  
Vol 14 ◽  
pp. 94-99 ◽  
Author(s):  
Zhongdian Dong ◽  
Pushun Chen ◽  
Ning Zhang ◽  
Shunkai Huang ◽  
Hairui Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Pcr Analysis ◽  
Quantitative Real Time Pcr ◽  
Oryzias Curvinotus

Gene Expression in Highly Sensitised Patients Waiting for a Kidney Transplant: A Real Time -PCR Analysis Approach

2012 ◽  
Vol 94 (10S) ◽  
pp. 1078
Author(s):  
R. I. Biticchi ◽  
A. Tagliamacco ◽  
U. Valente ◽  
I. Fontana ◽  
F. Ginevri ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Kidney Transplant ◽  
Real Time Pcr ◽  
Analysis Approach ◽  
Pcr Analysis

scholarly journals Selection and validation of reference genes for measuring gene expression in Toona ciliata under different experimental conditions by quantitative real-time PCR analysis

2020 ◽  
Author(s):  
Huiyun Song ◽  
Wenmai Mao ◽  
Zhihao Duan ◽  
Qingmin Que ◽  
Wei Zhou ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Reference Gene ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Accurate Method ◽  
Pcr Analysis ◽  
Quantitative Real Time Pcr ◽  
Experimental Conditions ◽  
Toona Ciliata

Abstract Background:Before studying gene expression of different organisms, it is important to determine the best reference gene. At present, the most accurate method of detecting gene expression is quantitative real-time PCR (RT-qPCR). With this method, reference genes that are stable in different biological systems and under different conditions can be obtained. Toona ciliata Roem ( T. ciliata ). is a valuable and fast-growing timber specie. In this study, 20 reference genes were identified using RT-qPCR, as a primary prerequisite for future gene expression analysis. Four different methods, geNorm, NormFinder, BestKeeper, and RankAggreg were used to evaluate the expression stability of the 20 candidate reference genes in various tissues under different conditions.Results:The experimental results showed that TUB-α was the most stably expressed reference gene across all samples and UBC17 was the most stable in leaves and young stems under Hypsipyla robusta ( H. robusta ) and methyl jasmonate (MeJA) treatments. In addition, PP2C59 and UBC5B were the best-performing genes in leaves under H. robusta treatment, while HIS1 and ACT7 were the best reference genes in young stems. The two best reference genes were 60S-18 and TUB-α after treatment at 4 °C. The expression of HIS6 and MUB1 was the most stable under PEG6000 treatment. The accuracy of the selected reference genes was verified using the transcription factor MYB3 ( TcMYB3) gene.Conclusions:This is the first report to verify the best reference genes for normalizing gene expression in T. ciliata under different conditions, which will facilitate future elucidation of gene regulations in this species.

Quantitative Real-Time PCR Analysis of Degradome Gene Expression

2008 ◽  
pp. 49-65
Author(s):  
Caroline J. Pennington ◽  
Robert K. Nuttall ◽  
Clara Sampieri-Ramirez ◽  
Matthew Wallard ◽  
Simon Pilgrim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Analysis ◽  
Quantitative Real Time Pcr

scholarly journals Microarray and real-time PCR analysis of adrenal gland gene expression in the 7-day-old rat: effects of hypoxia from birth

2007 ◽  
Vol 29 (2) ◽  
pp. 193-200 ◽  
Author(s):  
Eric D. Bruder ◽  
Julie J. Lee ◽  
Eric P. Widmaier ◽  
Hershel Raff
Keyword(s):  
Gene Expression ◽  
Food Intake ◽  
Real Time ◽  
Real Time Pcr ◽  
Plasma Corticosterone ◽  
Pcr Analysis ◽  
Rat Pups ◽  
Expression Of Genes ◽  
Adrenal Steroidogenesis ◽  
Induced Changes

We hypothesize that changes in adrenal gene expression mediate the increased plasma corticosterone and steroidogenesis in rat pups exposed to hypoxia from birth. In the current study, rat pups (with their dams) were exposed to hypoxia from birth and compared with pups from normoxic dams fed ad libitum or pair fed to match the decreased maternal food intake that occurs during hypoxia. Microarray analysis was performed, followed by verification with real-time PCR. Furthermore, the expression of selected genes involved in adrenal function was analyzed by real-time PCR, regardless of microarray results. Hypoxia increased plasma ACTH and corticosterone, while food restriction had no effect. Microarray revealed that many of the genes affected by hypoxia encode proteins that require molecular oxygen (monooxygenases, oxidoreductases, and electron transport), whereas only a few genes known to be involved in adrenal steroidogenesis were affected. Interestingly, the expression of genes involved in mitochondrial function and intermediary metabolism was increased by hypoxia. Real-time PCR detected a small but significant increase in the expression of Cyp21a1 mRNA in the hypoxic adrenal. When decreased maternal food intake was controlled for, the effects of hypoxia were more pronounced, in that real-time PCR detected significant increases in the expression of Star (244%), Cyp21a1 (208%), and Ldlr (233%). The present study revealed that increased plasma corticosterone in rat pups was due to hypoxia per se, and not as a result of decreased food intake by the hypoxic dam. Furthermore, hypoxia induced changes in gene expression that account for more productive and efficient steroidogenesis.

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