Characterization of cold-associated microRNAs in the freeze-tolerant gall fly Eurosta solidaginis using high-throughput sequencing

2016 ◽  
Vol 20 ◽  
pp. 95-100 ◽  
Author(s):  
Pierre J. Lyons ◽  
Louise Govaere ◽  
Nicolas Crapoulet ◽  
Kenneth B. Storey ◽  
Pier Jr Morin
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Eurosta Solidaginis ◽  
Freeze Tolerant

scholarly journals Characterization of oral bacterial diversity of irradiated patients by high-throughput sequencing

2013 ◽  
Vol 5 (1) ◽  
pp. 21-25 ◽  
Author(s):  
Yue-Jian Hu ◽  
Qian Wang ◽  
Yun-Tao Jiang ◽  
Rui Ma ◽  
Wen-Wei Xia ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
High Throughput ◽  
High Throughput Sequencing

Identification and characterization of salt-responsive microRNAs in Populus tomentosa by high-throughput sequencing

Biochimie ◽  
2013 ◽  
Vol 95 (4) ◽  
pp. 743-750 ◽  
Author(s):  
Yuanyuan Ren ◽  
Lei Chen ◽  
Yiyun Zhang ◽  
Xiangyang Kang ◽  
Zhiyi Zhang ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Populus Tomentosa ◽  
Identification And Characterization

scholarly journals Characterization of the Fecal Microbiota Using High-Throughput Sequencing Reveals a Stable Microbial Community during Storage

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e46953 ◽  
Author(s):  
Ian M. Carroll ◽  
Tamar Ringel-Kulka ◽  
Jennica P. Siddle ◽  
Todd R. Klaenhammer ◽  
Yehuda Ringel
Keyword(s):  
Microbial Community ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Fecal Microbiota

Molecular characterization of a new soybean-infecting member of the genus Nepovirus identified by high-throughput sequencing

2016 ◽  
Vol 162 (4) ◽  
pp. 1089-1092 ◽  
Author(s):  
Tuba Yasmin ◽  
Berlin D. Nelson ◽  
Houston A. Hobbs ◽  
Nancy K. McCoppin ◽  
Kris N. Lambert ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
High Throughput ◽  
High Throughput Sequencing

Characterization of microbial community in cold-chain hairtail fish by high-throughput sequencing technology

2021 ◽  
Author(s):  
Jiali Xing ◽  
Xiaorong Xu ◽  
Xiaohu Luo ◽  
Ruihang Zheng ◽  
Lingyan Mao ◽  
...  
Keyword(s):  
Microbial Community ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Bacterial Flora ◽  
Diversity Indices ◽  
Cold Chain ◽  
Class Level ◽  
The Family ◽  
Dominant Bacteria

Abstract: High-throughput sequencing was used to analyze the microbial communities in the muscle samples of hairtail fish to study their diversity and dynamic changes during cold-chain circulation. The results showed that the richness and diversity of the microbial community in hairtail fish had a transient decline in 0–24 h and decreased after the first rise during 24–216 h. The diversity and richness of bacteria in cold-chain hairtail fish reached the maximum at 168 h. The Shannon and Simpson diversity indices of the bacteria were 2.96 and 0.16, respectively, and their ACE and Chao1 richness indices were 254.84 and 155.10, respectively. In addition, the dominant bacteria were Proteobacteria in the phylum level, Gammaproteobacteria in the class level, Pseudomonadales in the order level, Pseudomonadaceae in the family level, and Pseudomonas in the genus level, and their relative abundance were 80.52%, 72.11%, 76.68%, 23.25%, and 53.50%, respectively. In this study, the structure of bacterial flora and the dominant bacteria in cold-chain hairtail fish were analyzed by high-throughput sequencing to provide a basis for exploring how to maintain the freshness of hairtail fish and for predicting the shelf-life of hairtail fish.

scholarly journals Characterization of Holstein and Normande whole milk miRNomes highlights breed specificities

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
S. Le Guillou ◽  
A. Leduc ◽  
J. Laubier ◽  
S. Barbey ◽  
M.-N. Rossignol ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Milk Production ◽  
High Throughput ◽  
Genetic Background ◽  
Molecular Mechanisms ◽  
High Throughput Sequencing ◽  
Whole Milk ◽  
Mammary Morphogenesis ◽  
Regulatory Properties

AbstractThe concept of milk as a healthy food has opened the way for studies on milk components, from nutrients to microRNAs, molecules with broad regulatory properties present in large quantities in milk. Characterization of these components has been performed in several species, such as humans and bovine, depending on the stages of lactation. Here, we have studied the variation in milk microRNA composition according to genetic background. Using high throughput sequencing, we have characterized and compared the milk miRNomes of Holstein and Normande cattle, dairy breeds with distinct milk production features, in order to highlight microRNAs that are essential for regulation of the lactation process. In Holstein and Normande milk, 2,038 and 2,030 microRNAs were identified, respectively, with 1,771 common microRNAs, of which 1,049 were annotated and 722 were predicted. The comparison of the milk miRNomes of two breeds allowed to highlight 182 microRNAs displaying significant differences in the abundance. They are involved in the regulation of lipid metabolism and mammary morphogenesis and development, which affects lactation. Our results provide new insights into the regulation of molecular mechanisms involved in milk production.

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