Identification of differentially expressed genes and quantitative expression of complement genes in the liver of marine medaka Oryzias melastigma challenged with Vibrio parahaemolyticus

2012 ◽  
Vol 7 (2) ◽  
pp. 191-200 ◽  
Author(s):  
Jun Bo ◽  
John P. Giesy ◽  
Rui Ye ◽  
Ke-Jian Wang ◽  
Jae-Seong Lee ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Vibrio Parahaemolyticus ◽  
Differentially Expressed ◽  
Marine Medaka ◽  
Quantitative Expression ◽  
Oryzias Melastigma

scholarly journals Transcriptome analysis of the induction of somatic embryogenesis in Coffea canephora and the participation of ARF and Aux/IAA genes

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7752 ◽  
Author(s):  
Ana O. Quintana-Escobar ◽  
Geovanny I. Nic-Can ◽  
Rosa María Galaz Avalos ◽  
Víctor M. Loyola-Vargas ◽  
Elsa Gongora-Castillo
Keyword(s):  
Somatic Embryogenesis ◽  
Signaling Pathway ◽  
Differentially Expressed Genes ◽  
Expression Profiles ◽  
Coffea Canephora ◽  
Differentially Expressed ◽  
Auxin Signaling ◽  
Quantitative Expression ◽  
Sequencing Technologies ◽  
Auxin Signaling Pathway

Background Somatic embryogenesis (SE) is a useful biotechnological tool to study the morpho-physiological, biochemical and molecular processes during the development of Coffea canephora. Plant growth regulators (PGR) play a key role during cell differentiation in SE. The Auxin-response-factor (ARF) and Auxin/Indole-3-acetic acid (Aux/IAA) are fundamental components involved in the signaling of the IAA. The IAA signaling pathway activates or represses the expression of genes responsive to auxins during the embryogenic transition of the somatic cells. The growing development of new generation sequencing technologies (NGS), as well as bioinformatics tools, has allowed us to broaden the landscape of SE study of various plant species and identify the genes directly involved. Methods Analysis of transcriptome expression profiles of the C. canephora genome and the identification of a particular set of differentially expressed genes (DEG) during SE are described in this study. Results A total of eight ARF and seven Aux/IAA differentially expressed genes were identified during the different stages of the SE induction process. The quantitative expression analysis showed that ARF18 and ARF5 genes are highly expressed after 21 days of the SE induction, while Aux/IAA7 and Aux/IAA12 genes are repressed. Discussion The results of this study allow a better understanding of the genes involved in the auxin signaling pathway as well as their expression profiles during the SE process.

scholarly journals Transcriptomic Analysis of Vibrio parahaemolyticus Reveals Different Virulence Gene Expression in Response to Benzyl Isothiocyanate

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 761 ◽  
Author(s):  
Jie Song ◽  
Hong-Man Hou ◽  
Hong-Yan Wu ◽  
Ke-Xin Li ◽  
Yan Wang ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Vibrio Parahaemolyticus ◽  
Molecular Mechanisms ◽  
Virulence Gene ◽  
Differentially Expressed ◽  
Transcriptional Analysis ◽  
Effective Control ◽  
Benzyl Isothiocyanate ◽  
Virulence Gene Expression ◽  
Sequencing Studies

Vibrio parahaemolyticus isolated from seafood is a pathogenic microorganism that leads to several acute diseases that are harmful to our health and is frequently transmitted by food. Therefore, there is an urgent need for the control and suppression of this pathogen. In this paper, transcriptional analysis was used to determine the effect of treatment with benzyl isothiocyanate (BITC) extracted from cruciferous vegetables on V. parahaemolyticus and to elucidate the molecular mechanisms underlying the response to BITC. Treatment with BITC resulted in 332 differentially expressed genes, among which 137 genes were downregulated, while 195 genes were upregulated. Moreover, six differentially expressed genes (DEGs) in RNA sequencing studies were further verified by quantitative real-time polymerase chain reaction (qRT-PCR). Genes found to regulate virulence encoded an l-threonine 3-dehydrogenase, a GGDEF family protein, the outer membrane protein OmpV, a flagellum-specific adenosine triphosphate synthase, TolQ protein and VirK protein. Hence, the results allow us to speculate that BITC may be an effective control strategy for inhibiting microorganisms growing in foods.

Differentially expressed genes in hemocytes of Litopenaeus vannamei challenged with Vibrio parahaemolyticus AHPND (VPAHPND) and VPAHPND toxin

2018 ◽  
Vol 81 ◽  
pp. 284-296 ◽  
Author(s):  
Benedict A. Maralit ◽  
Phattarunda Jaree ◽  
Pakpoom Boonchuen ◽  
Anchalee Tassanakajon ◽  
Kunlaya Somboonwiwat
Keyword(s):  
Differentially Expressed Genes ◽  
Litopenaeus Vannamei ◽  
Vibrio Parahaemolyticus ◽  
Differentially Expressed

cDNA-AFLP Analysis of Differentially Expressed Genes in Tobacco Infected by TMV

2013 ◽  
Vol 38 (1) ◽  
pp. 62-70 ◽  
Author(s):  
Rong-Ping CHEN ◽  
Lie LIU ◽  
Xiu-Qing WAN ◽  
En-Jian QIU ◽  
Chun-Jun WANG ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Differentially Expressed ◽  
Aflp Analysis
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