Molecular study of ovine glucose 6-phosphate dehydrogenase gene expression in respect to different energy intake

2010 ◽  
Vol 5 (2) ◽  
pp. 124-129 ◽  
Author(s):  
George P. Laliotis ◽  
Alkistis Vitsa ◽  
Iosif Bizelis ◽  
Maria A. Charismiadou ◽  
Emmanuel Rogdakis
Keyword(s):  
Gene Expression ◽  
Energy Intake ◽  
Molecular Study ◽  
Dehydrogenase Gene ◽  
Glucose 6 Phosphate Dehydrogenase

Effects of external phosphate concentration on glucose-6-phosphate dehydrogenase gene expression in the arbuscular mycorrhizal fungus Glomus intraradices

2006 ◽  
Vol 52 (9) ◽  
pp. 823-830 ◽  
Author(s):  
L I Stewart ◽  
S Jabaji-Hare ◽  
B T Driscoll
Keyword(s):  
Gene Expression ◽  
Mycorrhizal Fungi ◽  
Glomus Intraradices ◽  
Arbuscular Mycorrhizal Fungus ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Pentose Phosphate ◽  
Dehydrogenase Gene ◽  
Significant Difference ◽  
Glucose 6 Phosphate Dehydrogenase

Specific primers were developed to amplify a 227 bp segment of the arbuscular mycorrhizal fungus Glomus intraradices gene encoding glucose-6-phosphate dehydrogenase (G6PDH), an enzyme involved in the pentose phosphate pathway. G6PDH gene expression was measured by real-time quantitative reverse transcriptase – polymerase chain reaction in response to phosphorus (P) concentrations in the growth medium of colonized transformed carrot roots. We investigated the effects of different P concentration treatments on carbon (C) metabolism within the intraradical mycelia of G. intraradices. The results showed a significant (P = 0.017) down-regulation of G6PDH expression in the intraradical mycelia of G. intraradices cultures grown in high P than low P conditions but no significant difference in regulation in excessive P concentrations when compared with the low P or high P concentrations. These results indicate that a reduction in the C flow from the host could be occurring as a result of elevated P and that a decrease in fungal G6PDH gene expression occurs, but not in the short term (less than 2 h). Reduced C flow from the host could lead to reduced fungal growth and root colonization, as was observed under high soil P conditions.Key words: arbuscular mycorrhizal fungi, phosphorus, nutrient uptake, glucose-6-phosphate dehydrogenase, gene expression.

scholarly journals Malic enzyme and glucose 6-phosphate dehydrogenase gene expression increases in rat liver cirrhogenesis

1997 ◽  
Vol 75 (4) ◽  
pp. 487-492 ◽  
Author(s):  
N Sanz ◽  
C Díez-Fernández ◽  
AM Valverde ◽  
M Lorenzo ◽  
M Benito ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
Malic Enzyme ◽  
Dehydrogenase Gene ◽  
Glucose 6 Phosphate Dehydrogenase

scholarly journals Induction of the glucose-6-phosphate dehydrogenase gene expression by chronic hypoxia in PC12 cells

FEBS Letters ◽  
2004 ◽  
Vol 569 (1-3) ◽  
pp. 256-260 ◽  
Author(s):  
Lin Gao ◽  
Rebeca Mejı́as ◽  
Miriam Echevarrı́a ◽  
José López-Barneo
Keyword(s):  
Gene Expression ◽  
Pc12 Cells ◽  
Chronic Hypoxia ◽  
Dehydrogenase Gene ◽  
Glucose 6 Phosphate Dehydrogenase

scholarly journals Effect of dithiocyano-methane on hexose monophosphate pathway in the respiratory metabolism of Escherichia coli

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yanfeng Chen ◽  
Wenjie Ke ◽  
Huabin Qin ◽  
Siwei Chen ◽  
Limei Qin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Control Group ◽  
Respiratory Metabolism ◽  
Hexose Monophosphate ◽  
Hexose Monophosphate Pathway ◽  
Response To Stress ◽  
Glucose Decomposition ◽  
Decomposition Pathway ◽  
Glucose 6 Phosphate Dehydrogenase

Abstract This paper studied the inhibitory effects of dithiocyano-methane (DM) on the glucose decomposition pathway in the respiratory metabolism of Escherichia coli. We investigated the effects of DM on the activities of key enzymes (ATPase and glucose-6-phosphate dehydrogenase, G6PDH), the levels of key product (nicotinamide adenosine denucleotide hydro-phosphoric acid, NADPH), and gene expression in the hexose monophosphate pathway (HMP). The results showed that the minimum inhibitory concentration (MIC) and the minimum bactericide concentration (MBC) of DM against the tested strains were 5.86 mg/L and 11.72 mg/L, respectively. Bacteria exposed to DM at MIC demonstrated an increase in bacterial ATPase and G6PDH activities, NADPH levels, and gene expression in the HMP pathway compared to bacteria in the control group, which could be interpreted as a behavioral response to stress introduced by DM. However, DM at a lethal concentration of 10 × MIC affected glucose decomposition by inhibiting mainly the HMP pathway in E. coli.

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