Proteomic analysis of the neutrophil proteins of the tammar wallaby (Macropus eugenii)

2006 ◽  
Vol 1 (3) ◽  
pp. 283-291 ◽  
Author(s):  
Kiran Ambatipudi ◽  
Julie Old ◽  
Michael Guilhaus ◽  
Mark Raftery ◽  
Lyn Hinds ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Tammar Wallaby ◽  
Macropus Eugenii

scholarly journals Growth of the corpus luteum and its progesterone content during pregnancy in the tammar wallaby, Macropus eugenii

Reproduction ◽  
1979 ◽  
Vol 57 (1) ◽  
pp. 131-136 ◽  
Author(s):  
M. B. Renfree ◽  
S. W. Green ◽  
I. R. Young
Keyword(s):  
Corpus Luteum ◽  
Tammar Wallaby ◽  
Macropus Eugenii

Mullerian inhibiting substance production and testicular migration and descent in the pouch young of a marsupial

Development ◽  
1988 ◽  
Vol 104 (4) ◽  
pp. 549-556 ◽  
Author(s):  
J.M. Hutson ◽  
G. Shaw ◽  
W.S. O ◽  
R.V. Short ◽  
M.B. Renfree
Keyword(s):  
Tammar Wallaby ◽  
Testicular Descent ◽  
Future Studies ◽  
Macropus Eugenii ◽  
Müllerian Inhibiting Substance ◽  
Sequence Of Events ◽  
Testicular Differentiation ◽  
Testicular Migration ◽  
Australian Marsupial ◽  
Mullerian Inhibiting Substance

The ontogeny of Mullerian inhibiting substance (MIS) production by the developing testis of an Australian marsupial, the tammar wallaby (Macropus eugenii), was determined during pouch life using an organ-culture bioassay of mouse fetal urogenital ridge. This information was related to the morphological events during testicular migration and descent. MIS biological activity was found in testes (but not ovaries or liver) of pouch young from 2 to 85 days of age. MIS production had commenced by day 2, which is within a day of the first gross morphological signs of testicular differentiation. Mullerian duct regression occurred between 10 and 30 days, which partly coincided with testicular migration to the inguinal region and enlargement of the gubernacular bulb (15 to 30 days). These observations are consistent with the hypothesis that MIS may be involved in testicular transabdominal migration. The epididymis commenced development and growth only after the testis had descended through the inguinal ring. This provides no support for the suggestion that the epididymis is involved in testicular descent into the scrotum. The basic sequence of events in post-testicular sexual differentiation in the wallaby is sufficiently similar to that seen in eutherian mammals to make it an excellent experimental model for future studies of testicular differentiation, migration and descent.

Biochemical studies of intrauterine components of the tammar wallaby Macropus eugenii during pregnancy

Development ◽  
1981 ◽  
Vol 62 (1) ◽  
pp. 325-338
Author(s):  
Elizabeth J. Thornber ◽  
Marilyn B. Renfree ◽  
Gregory I. Wallace
Keyword(s):  
Protein Concentration ◽  
Specific Binding ◽  
Polyacrylamide Gel Electrophoresis ◽  
Fold Increase ◽  
Tammar Wallaby ◽  
Macropus Eugenii ◽  
Tenfold Increase ◽  
Specific Proteins ◽  
Wet Weight

The in vitro uptake and incorporation of [3H]ui idine by blastocysts of the tammar wallaby showed a 16- and 30-fold increase from day 0 to day 10 after removal of pouch young, respectively. Two of the six non-expanded blastocysts recovered on day 5 showed a tenfold increase in incorporation. During the first ten days after removal of pouch young the diameter of the blastocyst increased threefold. Endometrial exudate from gravid uteri had a higher protein concentration than exudate from nongravid uteri (39·5 ± 0·9 and 32·0 ± 2·0 mg/ml (mean ± s.e.m.), respectively). Endometrial exudates from uteri where the blastocyst was actively growing were found to contain six uterine-specific proteins. These were separated by gradient polyacrylamide gel electrophoresis. Two of the proteins were pre-albumins and the others were larger molecules (M.W. 153000–670000). Two proteins were only present at particular stages of pregnancy: the other four were present at all stages from diapause to birth, in exudate from gravid and nongravid uteri. The specific binding of progesterone and androstenedione to proteins in endometrial exudates or uterine flushings from pregnant wallabies was less than one per cent of the value obtained from day-5 pregnant rabbits. The ability of mouse blastocysts to take up and incorporate [3H]uridine into acidinsoluble material increased threefold in the presence of day-10 endometrial exudates from wallabies. However, this was less than ten percent of the values obtained in the presence of bovine serum albumin. The concentration of calcium in endometrial exudates increased from 23·6 to 45·2 μg/ml during pregnancy; in endometrium it remained at 88·7 μg/g (wet weight) throughout pregnancy, and in plasma it was 53·3 μg/ml. The concentration of zinc in endometrial exudates was 4·5 μg/ml; in endometrium it decreased from 21·8 to 13·3 μg/g (wet weight) during pregnancy and in plasma it was 0·6 μg/ml.

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