Cardiac Regeneration by Intrinsic Cardiac Stem Cells

2011 ◽  
Vol 17 (9) ◽  
pp. S130
Author(s):  
Toshio Nagai ◽  
Masato Kanda ◽  
Meilan Liu ◽  
Naomichi Kondo ◽  
Toshinao Takahashi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cardiac Regeneration ◽  
Cardiac Stem Cells

Harnessing endogenous intra- and extra-cardiac stem cells for cardiac regeneration – hope or hype?

2009 ◽  
Vol 6 (4) ◽  
pp. 127-133 ◽  
Author(s):  
Sunny Sun-Kin Chan ◽  
Ying-Zhang Shueh ◽  
Yen-Wen Liu ◽  
Patrick C.H. Hsieh
Keyword(s):  
Stem Cells ◽  
Cardiac Regeneration ◽  
Cardiac Stem Cells

Medical imaging for cardiac regeneration using resident cardiac stem cells

2013 ◽  
Vol 5 (5) ◽  
pp. 477-486
Author(s):  
Sung Hyun Choi ◽  
So Young Yoo ◽  
Sae Mi Yoo ◽  
Kyeong Been Lee ◽  
Sang Hong Baek ◽  
...  
Keyword(s):  
Stem Cells ◽  
Medical Imaging ◽  
Cardiac Regeneration ◽  
Cardiac Stem Cells

scholarly journals Adult c-kitpos Cardiac Stem Cells Are Necessary and Sufficient for Functional Cardiac Regeneration and Repair

Cell ◽  
2013 ◽  
Vol 154 (4) ◽  
pp. 827-842 ◽  
Author(s):  
Georgina M. Ellison ◽  
Carla Vicinanza ◽  
Andrew J. Smith ◽  
Iolanda Aquila ◽  
Angelo Leone ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cardiac Regeneration ◽  
Cardiac Stem Cells ◽  
Necessary And Sufficient

scholarly journals Dormant cardiac stem cells: A promising tool in cardiac regeneration

2020 ◽  
Author(s):  
Zenaida Ceausu ◽  
Bogdan Socea ◽  
Mihai Dimitriu ◽  
Dragoş Predescu ◽  
Vlad Constantin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cardiac Regeneration ◽  
Cardiac Stem Cells ◽  
Promising Tool

Abstract 13831: Atrial and Ventricular Cardiac Stem Cells From the Same Heart Exhibit Differential Functional Characteristics for Cardiac Regeneration but Are Superior to the Endothelial Progenitor Cells From the Same Patient

Circulation ◽  
2015 ◽  
Vol 132 (suppl_3) ◽  
Author(s):  
Parul Dixit ◽  
Hayden Donnelly ◽  
Midori Edamatsu ◽  
Ivor Galvin ◽  
Richard Bunton ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Death ◽  
Progenitor Cells ◽  
Wound Closure ◽  
Apoptotic Cell ◽  
Cardiac Regeneration ◽  
Cardiac Stem Cells ◽  
Cell Type ◽  
Functional Characteristics ◽  
Scratch Assay

Introduction: Deciding the best cell type for cardiac regeneration remains a challenge, however, no studies have directly compared the functional efficacy of cardiac stem cells (CSCs) with extra- cardiac SCs isolated from the same patient. Objective: To compare the functional characteristics of endothelial progenitor cells (EPCs), right atrial (RAA) CSCs and left ventricular (LV) CSCs isolated from the same patients. Methods and Results: Flow-cytometry analysis of CSCs (n=12 patients) revealed higher expression of CD90 (involved in cell adhesion and migration) in RAA CSCs (CD90 & CD105 ++ve cells: 71±15% in RAA vs. 47±27% in LV, P<0.05). However, scratch assay showed superior wound closure in LV CSCs (80±10% in LV vs. 60±5% in RAA, P<0.05) suggesting that LV CSCs have a better migration potential. Moreover, the expansion properties of RAA CSCs were better than LV CSCs in terms of higher cumulative population doublings in culture. To simulate in vivo ischemic environment, cells were subjected to hypoxia (1% O2) and serum starvation for 3 days. RAA and LV CSCs exhibited similar pattern of resistance to apoptotic cell death (evaluated as percentage of annexin +ve cells, n=5) and proliferation under hypoxia. Interestingly, EPCs exhibited highest resistance to apoptotic cell death, but they also showed the lowest proliferation under hypoxia (P<0.05). Secretion of paracrine and pro-angiogenic factors have been thought to be most important mechanisms behind cardiac regeneration. Therefore, to compare the paracrine angiogenic and wound healing potential, HUVECs were cultured in culture supernatant (CS) from all the 3 cell types. RAA-CS showed the highest increase in tube formation capacity (P<0.05, n=6), while the scratch assay showed comparable wound closure with both LV and RAA CS (67.56 ± 7.36% and 70.02 ± 5.70%, n=5), but was lowest in EPC CS (54±7%). The secretion of the angiogenic growth factor VEGF-A (n=5) was lowest in EPCs (0.36± 0.20 ng/mL) and comparable between RAA (3.44±1.94 ng/mL) and LV CSCs (4.33± 1.57 ng/mL). Conclusion: Stem cells from different regions exhibit differential functional characteristics within the same patient. The selection of the best cell type should therefore be influenced by the nature of the injury and the type of effect desired.

scholarly journals Promotion of Cardiac Regeneration by Cardiac Stem Cells

2005 ◽  
Vol 97 (7) ◽  
pp. 615-617 ◽  
Author(s):  
Toshio Nagai ◽  
Ichiro Shiojima ◽  
Katsuhisa Matsuura ◽  
Issei Komuro
Keyword(s):  
Stem Cells ◽  
Cardiac Regeneration ◽  
Cardiac Stem Cells

Innovation in Basic Science: Stem Cells and their role in the treatment of Paediatric Cardiac Failure – Opportunities and Challenges

2009 ◽  
Vol 19 (S2) ◽  
pp. 74-84 ◽  
Author(s):  
Sunjay Kaushal ◽  
Jeffrey Phillip Jacobs ◽  
Jeffrey G. Gossett ◽  
Ann Steele ◽  
Peter Steele ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Embryonic Stem Cells ◽  
Cardiac Failure ◽  
Adult Stem Cells ◽  
Embryonic Stem ◽  
Cardiac Regeneration ◽  
Cardiac Progenitor Cells ◽  
Cardiac Stem Cells ◽  
Skeletal Myoblasts

AbstractHeart failure is a leading cause of death worldwide. Current therapies only delay progression of the cardiac disease or replace the diseased heart with cardiac transplantation. Stem cells represent a recently discovered novel approach to the treatment of cardiac failure that may facilitate the replacement of diseased cardiac tissue and subsequently lead to improved cardiac function and cardiac regeneration.A stem cell is defined as a cell with the properties of being clonogenic, self-renewing, and multipotent. In response to intercellular signalling or environmental stimuli, stem cells differentiate into cells derived from any of the three primary germ layers: ectoderm, endoderm, and mesoderm, a powerful advantage for regenerative therapies. Meanwhile, a cardiac progenitor cell is a multipotent cell that can differentiate into cells of any of the cardiac lineages, including endothelial cells and cardiomyocytes.Stem cells can be classified into three categories: (1) adult stem cells, (2) embryonic stem cells, and (3) induced pluripotential cells. Adult stem cells have been identified in numerous organs and tissues in adults, including bone-marrow, skeletal muscle, adipose tissue, and, as was recently discovered, the heart. Embryonic stem cells are derived from the inner cell mass of the blastocyst stage of the developing embryo. Finally through transcriptional reprogramming, somatic cells, such as fibroblasts, can be converted into induced pluripotential cells that resemble embryonic stem cells.Four classes of stem cells that may lead to cardiac regeneration are: (1) Embryonic stem cells, (2) Bone Marrow derived stem cells, (3) Skeletal myoblasts, and (4) Cardiac stem cells and cardiac progenitor cells. Embryonic stem cells are problematic because of several reasons: (1) the formation of teratomas, (2) potential immunologic cellular rejection, (3) low efficiency of their differentiation into cardiomyocytes, typically 1% in culture, and (4) ethical and political issues. As of now, bone marrow derived stem cells have not been proven to differentiate reproducibly and reliably into cardiomyocytes. Skeletal myoblasts have created in vivo myotubes but have not electrically integrated with the myocardium. Cardiac stem cells and cardiac progenitor cells represent one of the most promising types of cellular therapy for children with cardiac failure.

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