Inhibition of DNA-dependent protein kinase promotes ultrasound-induced cell death including apoptosis in human leukemia cells

2012 ◽  
Vol 322 (1) ◽  
pp. 107-112 ◽  
Author(s):  
Yukihiro Furusawa ◽  
Yoshisada Fujiwara ◽  
Mariame Ali Hassan ◽  
Yoshiaki Tabuchi ◽  
Akinori Morita ◽  
...  
Keyword(s):  
Cell Death ◽  
Protein Kinase ◽  
Leukemia Cells ◽  
Human Leukemia ◽  
Dependent Protein Kinase ◽  
Human Leukemia Cells ◽  
Dependent Protein

scholarly journals Inhibition of the NAD-Dependent Protein Deacetylase SIRT2 Induces Granulocytic Differentiation in Human Leukemia Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e57633 ◽  
Author(s):  
Yoshitaka Sunami ◽  
Marito Araki ◽  
Yumi Hironaka ◽  
Soji Morishita ◽  
Masaki Kobayashi ◽  
...  
Keyword(s):  
Leukemia Cells ◽  
Human Leukemia ◽  
Granulocytic Differentiation ◽  
Human Leukemia Cells ◽  
Dependent Protein ◽  
Protein Deacetylase

Melatonin enhances hyperthermia-induced apoptotic cell death in human leukemia cells

2016 ◽  
Vol 61 (3) ◽  
pp. 381-395 ◽  
Author(s):  
Carlos Quintana ◽  
Javier Cabrera ◽  
Juan Perdomo ◽  
Francisco Estévez ◽  
Juan F. Loro ◽  
...  
Keyword(s):  
Cell Death ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Leukemia Cells ◽  
Human Leukemia ◽  
Human Leukemia Cells

scholarly journals Naja atra Cardiotoxin 3 Elicits Autophagy and Apoptosis in U937 Human Leukemia Cells through the Ca2+/PP2A/AMPK Axis

Toxins ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 527 ◽  
Author(s):  
Jing-Ting Chiou ◽  
Yi-Jun Shi ◽  
Liang-Jun Wang ◽  
Chia-Hui Huang ◽  
Yuan-Chin Lee ◽  
...  
Keyword(s):  
Cell Death ◽  
Membrane Damage ◽  
Leukemia Cells ◽  
Human Leukemia ◽  
U937 Cells ◽  
Cell Membrane Damage ◽  
Human Leukemia Cells ◽  
Phosphatase 2A ◽  
Induced Apoptosis ◽  
Naja Atra

Cardiotoxins (CTXs) are suggested to exert their cytotoxicity through cell membrane damage. Other studies show that penetration of CTXs into cells elicits mitochondrial fragmentation or lysosome disruption, leading to cell death. Considering the role of AMPK-activated protein kinase (AMPK) in mitochondrial biogenesis and lysosomal biogenesis, we aimed to investigate whether the AMPK-mediated pathway modulated Naja atra (Taiwan cobra) CTX3 cytotoxicity in U937 human leukemia cells. Our results showed that CTX3 induced autophagy and apoptosis in U937 cells, whereas autophagic inhibitors suppressed CTX3-induced apoptosis. CTX3 treatment elicited Ca2+-dependent degradation of the protein phosphatase 2A (PP2A) catalytic subunit (PP2Acα) and phosphorylation of AMPKα. Overexpression of PP2Acα mitigated the CTX3-induced AMPKα phosphorylation. CTX3-induced autophagy was via AMPK-mediated suppression of the Akt/mTOR pathway. Removal of Ca2+ or suppression of AMPKα phosphorylation inhibited the CTX3-induced cell death. CTX3 was unable to induce autophagy and apoptosis in U937 cells expressing constitutively active Akt. Met-modified CTX3 retained its membrane-perturbing activity, however, it did not induce AMPK activation and death of U937 cells. These results conclusively indicate that CTX3 induces autophagy and apoptosis in U937 cells via the Ca2+/PP2A/AMPK axis, and suggest that the membrane-perturbing activity of CTX3 is not crucial for the cell death signaling pathway induction.

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