Temporal changes in the response of SVZ neural stem cells to intraventricular administration of growth factors

2016 ◽  
Vol 1636 ◽  
pp. 118-129 ◽  
Author(s):  
Takashi Ochi ◽  
Hirofumi Nakatomi ◽  
Akihiro Ito ◽  
Hideaki Imai ◽  
Shigeo Okabe ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Neural Stem Cells ◽  
Temporal Changes ◽  
Intraventricular Administration

scholarly journals Differentiation of Neural Stem Cells Into Cells of Oligodendroglial Lineage

2007 ◽  
Vol 50 (1) ◽  
pp. 35-41
Author(s):  
Jaroslav Mokrý ◽  
Jana Karbanová ◽  
Dana Čížková ◽  
Jan Pazour ◽  
Stanislav Filip ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Cell Differentiation ◽  
Neural Stem Cells ◽  
Developmental Stages ◽  
Developmental Studies ◽  
Differentiation Markers ◽  
Lineage Differentiation ◽  
Massive Cell Death

We described three different conditions that induce differentiation of dissociated neural stem cells derived from mouse embryonic CNS. In the first set of experiments, where the cell differentiation was triggered by cell adhesion, removal of growth factors and serum-supplemented medium, only sporadic neuronal and astroglial cells survived longer than two weeks and the latter formed a monolayer. When differentiation was induced in serum-free medium supplemented with retinoic acid, rapid and massive cell death occurred. A prolonged survival was observed in cultivation medium supplemented with serum and growth factors EGF plus FGF-2. One third of the cells did not express cell differentiation markers and were responsible for an increase in cell numbers. The remaining cells differentiated and formed the astrocytic monolayer on which occasional neuronal cells grew. One third of the differentiated phenotypes were represented by cells of oligodendroglial lineage. Differentiation of oligodendroglial cells occurred in a stepwise mechanism because the culture contained all successive developmental stages, including oligodendrocyte progenitors, preoligodendrocytes and immature and mature oligodendrocytes. Maturing oligodendrocytes displayed immunocytochemical and morphological features characteristic of cells that undergo physiological development. The cultivation conditions that supported growth and differentiation of neural stem cells were optimal for in vitro developmental studies and the production of oligodendroglial cells.

Tethered growth factors on biocompatible scaffolds improve stemness of cultured rat and human neural stem cells and growth of oligodendrocyte progenitors

Methods ◽  
2018 ◽  
Vol 133 ◽  
pp. 54-64 ◽  
Author(s):  
Lisamarie Moore ◽  
Nolan B. Skop ◽  
Deborah E. Rothbard ◽  
Lucas R. Corrubia ◽  
Steven W. Levison
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Neural Stem Cells ◽  
Oligodendrocyte Progenitors ◽  
Human Neural Stem Cells

scholarly journals Cross Talk between Notch and Growth Factor/Cytokine Signaling Pathways in Neural Stem Cells

2007 ◽  
Vol 27 (11) ◽  
pp. 3982-3994 ◽  
Author(s):  
Motoshi Nagao ◽  
Michiya Sugimori ◽  
Masato Nakafuku
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Growth Factor ◽  
Neural Stem Cells ◽  
Signaling Pathways ◽  
Intracellular Signaling ◽  
The Self ◽  
Cell Surface Receptor ◽  
Cytokine Signaling ◽  
Self Renewal

ABSTRACT Precise control of proliferation and differentiation of multipotent neural stem cells (NSCs) is crucial for proper development of the nervous system. Although signaling through the cell surface receptor Notch has been implicated in many aspects of neural development, its role in NSCs remains elusive. Here we examined how the Notch pathway cross talks with signaling for growth factors and cytokines in controlling the self-renewal and differentiation of NSCs. Both Notch and growth factors were required for active proliferation of NSCs, but each of these signals was sufficient and independent of the other to inhibit differentiation of neurons and glia. Moreover, Notch signals could support the clonal self-renewing growth of NSCs in the absence of growth factors. This growth factor-independent action of Notch involved the regulation of the cell cycle and cell-cell interactions. During differentiation of NSCs, Notch signals promoted the generation of astrocytes in collaboration with ciliary neurotrophic factor and growth factors. Their cooperative actions were likely through synergistic phosphorylation of signal transducer and activator of transcription 3 on tyrosine at position 705 and serine at position 727. Our data suggest that distinct intracellular signaling pathways operate downstream of Notch for the self-renewal of NSCs and stimulation of astrogenesis.

scholarly journals A network of heterochronic genes including Imp1 regulates temporal changes in stem cell properties

eLife ◽  
2013 ◽  
Vol 2 ◽  
Author(s):  
Jinsuke Nishino ◽  
Sunjung Kim ◽  
Yuan Zhu ◽  
Hao Zhu ◽  
Sean J Morrison
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Neural Stem Cells ◽  
Adult Stem Cells ◽  
Rna Binding ◽  
Cell Function ◽  
Temporal Changes ◽  
Evolutionarily Conserved ◽  
Heterochronic Gene ◽  
Heterochronic Genes

Stem cell properties change over time to match the changing growth and regeneration demands of tissues. We showed previously that adult forebrain stem cell function declines during aging because of increased expression of let-7 microRNAs, evolutionarily conserved heterochronic genes that reduce HMGA2 expression. Here we asked whether let-7 targets also regulate changes between fetal and adult stem cells. We found a second let-7 target, the RNA binding protein IMP1, that is expressed by fetal, but not adult, neural stem cells. IMP1 expression was promoted by Wnt signaling and Lin28a expression and opposed by let-7 microRNAs. Imp1-deficient neural stem cells were prematurely depleted in the dorsal telencephalon due to accelerated differentiation, impairing pallial expansion. IMP1 post-transcriptionally inhibited the expression of differentiation-associated genes while promoting the expression of self-renewal genes, including Hmga2. A network of heterochronic gene products including Lin28a, let-7, IMP1, and HMGA2 thus regulates temporal changes in stem cell properties.

scholarly journals Rat Hippocampal Neural Stem Cell Modulation Using PDGF, VEGF, PDGF/VEGF, and BDNF

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Neus Gomila Pelegri ◽  
Catherine A. Gorrie ◽  
Jerran Santos
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factors ◽  
Growth Factor ◽  
Neural Stem Cells ◽  
Platelet Derived Growth Factor ◽  
Vascular Endothelial ◽  
Cord Injury ◽  
Endothelial Growth Factor

Neural stem cells have become the focus of many studies as they have the potential to differentiate into all three neural lineages. This may be utilised to develop new and novel ways to treat neurological conditions such as spinal cord and brain injuries, especially if the stem cells can be modulated in vivo without additional invasive surgical procedures. This research is aimed at investigating the effects of the growth factors vascular endothelial growth factor, platelet-derived growth factor, brain-derived neurotrophic factor, and vascular endothelial growth factor/platelet-derived growth factor on hippocampal-derived neural stem cells. Cell growth and differentiation were assessed using immunohistochemistry and glutaminase enzyme assay. Cells were cultured for 14 days and treated with different growth factors at two different concentrations 20 ng/mL and 100 ng/mL. At 2 weeks, cells were fixed, and immunohistochemistry was conducted to determine cellular differentiation using antibodies against GFAP, nestin, OSP, and NF200. The cell medium supernatant was also collected during treatment to determine glutaminase levels secreted by the cells as an indicator of neural differentiation. VEGF/PDGF at 100 ng/mL had the greatest influence on cellular proliferation of HNSC, which also stained positively for nestin, OSP, and NF200. In comparison, HNSC in other treatments had poorer cell health and adhesion. HNSC in all treatment groups displayed some differentiation markers and morphology, but this is most significant in the 100 ng/ml VEGF/PDGF treatment. VEGF/PDGF combination produced the optimal effect on the HNSCs inducing the differentiation pathway exhibiting oligodendrocytic and neuronal markers. This is a promising finding that should be further investigated in the brain and spinal cord injury.

scholarly journals Controlled release of dextrin-conjugated growth factors to support growth and differentiation of neural stem cells

2018 ◽  
Vol 33 ◽  
pp. 69-78 ◽  
Author(s):  
Elaine L. Ferguson ◽  
Sameza Naseer ◽  
Lydia C. Powell ◽  
Joseph Hardwicke ◽  
Fraser I. Young ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Controlled Release ◽  
Neural Stem Cells
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