Alpha-lipoic acid pre- and post-treatments provide protection against in vitro ischemia-reperfusion injury in cerebral endothelial cells via Akt/mTOR signaling

2012 ◽  
Vol 1482 ◽  
pp. 81-90 ◽  
Author(s):  
Rong Xie ◽  
Xiaomu Li ◽  
Yan Ling ◽  
Chao Shen ◽  
Xing Wu ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Reperfusion Injury ◽  
Lipoic Acid ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Mtor Signaling ◽  
Alpha Lipoic Acid ◽  
Cerebral Endothelial Cells ◽  
In Vitro Ischemia

scholarly journals Inhibition of BRD4 Reduces Neutrophil Activation and Adhesion to the Vascular Endothelium Following Ischemia Reperfusion Injury

2020 ◽  
Vol 21 (24) ◽  
pp. 9620
Author(s):  
Shelby Reid ◽  
Noah Fine ◽  
Vikrant K. Bhosle ◽  
Joyce Zhou ◽  
Rohan John ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Neutrophil Adhesion ◽  
Neutrophil Accumulation ◽  
Iri Model ◽  
Brd4 Inhibition

Renal ischemia reperfusion injury (IRI) is associated with inflammation, including neutrophil infiltration that exacerbates the initial ischemic insult. The molecular pathways involved are poorly characterized and there is currently no treatment. We performed an in silico analysis demonstrating changes in NFκB-mediated gene expression in early renal IRI. We then evaluated NFκB-blockade with a BRD4 inhibitor on neutrophil adhesion to endothelial cells in vitro, and tested BRD4 inhibition in an in vivo IRI model. BRD4 inhibition attenuated neutrophil adhesion to activated endothelial cells. In vivo, IRI led to increased expression of cytokines and adhesion molecules at 6 h post-IRI with sustained up-regulated expression to 48 h post-IRI. These effects were attenuated, in part, with BRD4 inhibition. Absolute neutrophil counts increased significantly in the bone marrow, blood, and kidney 24 h post-IRI. Activated neutrophils increased in the blood and kidney at 6 h post-IRI and remained elevated in the kidney until 48 h post-IRI. BRD4 inhibition reduced both total and activated neutrophil counts in the kidney. IRI-induced tubular injury correlated with neutrophil accumulation and was reduced by BRD4 inhibition. In summary, BRD4 inhibition has important systemic and renal effects on neutrophils, and these effects are associated with reduced renal injury.

scholarly journals The Protective Effects of Alpha-Lipoic Acid and Coenzyme Q10Combination on Ovarian Ischemia-Reperfusion Injury: An Experimental Study

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Ahmet Ali Tuncer ◽  
Mehmet Fatih Bozkurt ◽  
Tulay Koken ◽  
Nurhan Dogan ◽  
Mine Kanat Pektaş ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Reperfusion Injury ◽  
Lipoic Acid ◽  
Sham Group ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Ovarian Tissue ◽  
Coenzyme Q ◽  
Alpha Lipoic Acid ◽  
Oxide Synthase

Objective. This study aims to evaluate whether alpha-lipoic acid and/or coenzyme Q10can protect the prepubertal ovarian tissue from ischemia-reperfusion injury in an experimental rat model of ovarian torsion.Materials and Methods. Forty-two female preadolescent Wistar-Albino rats were divided into 6 equal groups randomly. The sham group had laparotomy without torsion; the other groups had torsion/detorsion procedure. After undergoing torsion, group 2 received saline, group 3 received olive oil, group 4 received alpha-lipoic acid, group 5 received coenzyme Q10, and group 6 received both alpha-lipoic acid and coenzyme Q10orally. The oxidant-antioxidant statuses of these groups were compared using biochemical measurement of oxidized/reduced glutathione, glutathione peroxidase and malondialdehyde, pathological evaluation of damage and apoptosis within the ovarian tissue, and immunohistochemical assessment of nitric oxide synthase.Results. The left ovaries of the alpha-lipoic acid + coenzyme Q10group had significantly lower apoptosis scores and significantly higher nitric oxide synthase content than the left ovaries of the control groups. The alpha-lipoic acid + coenzyme Q10group had significantly higher glutathione peroxidase levels and serum malondialdehyde concentrations than the sham group.Conclusions. The combination of alpha-lipoic acid and coenzyme Q10has beneficial effects on oxidative stress induced by ischemia-reperfusion injury related to ovarian torsion.

Vascular reactivity in old spontaneously hypertensive stroke-prone rats- effects of in vitro ischemia/reperfusion injury

2021 ◽  
Vol 04 (03) ◽  
Author(s):  
Sevil Korkmaz-Icöz ◽  
Mona Isabella Benker ◽  
Shiliang Li ◽  
Sivakkanan Loganathan ◽  
Patricia Kraft ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Vascular Reactivity ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Spontaneously Hypertensive ◽  
In Vitro Ischemia

A novel in vitro ischemia/reperfusion injury model

2009 ◽  
Vol 32 (3) ◽  
pp. 421-429 ◽  
Author(s):  
Won Hee Lee ◽  
Sungkwon Kang ◽  
Pavlos P. Vlachos ◽  
Yong Woo Lee
Keyword(s):  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
In Vitro Ischemia ◽  
Injury Model

Alpha-lipoic acid provides neuroprotection from ischemia-reperfusion injury of peripheral nerve

1999 ◽  
Vol 163 (1) ◽  
pp. 11-16 ◽  
Author(s):  
Yoshiyuki Mitsui ◽  
James D. Schmelzer ◽  
Paula J. Zollman ◽  
Manami Mitsui ◽  
Hans J. Tritschler ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Reperfusion Injury ◽  
Lipoic Acid ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Alpha Lipoic Acid

Neuroprotective effect of alpha-lipoic acid and methylprednisolone on the spinal cord ischemia/reperfusion injury in rabbits

2014 ◽  
Vol 29 (1) ◽  
pp. 46-51 ◽  
Author(s):  
Mehmet Gazi Boyaci ◽  
Olcay Eser ◽  
Cevdet Ugur Kocogullari ◽  
Ergun Karavelioglu ◽  
Cigdem Tokyol ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Reperfusion Injury ◽  
Lipoic Acid ◽  
Ischemia Reperfusion Injury ◽  
Neuroprotective Effect ◽  
Ischemia Reperfusion ◽  
Spinal Cord Ischemia ◽  
Alpha Lipoic Acid
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