Cathepsin L is involved in 6-hydroxydopamine induced apoptosis of SH-SY5Y neuroblastoma cells

2011 ◽  
Vol 1387 ◽  
pp. 29-38 ◽  
Author(s):  
Bei Xiang ◽  
Xifeng Fei ◽  
Wenzhuo Zhuang ◽  
Yun Fang ◽  
Zhenghong Qin ◽  
...  
Keyword(s):  
Cathepsin L ◽  
Neuroblastoma Cells ◽  
Induced Apoptosis ◽  
6 Hydroxydopamine

scholarly journals MycN sensitizes neuroblastoma cells for drug-induced apoptosis

Oncogene ◽  
1999 ◽  
Vol 18 (7) ◽  
pp. 1479-1486 ◽  
Author(s):  
Simone Fulda ◽  
Werner Lutz ◽  
Manfred Schwab ◽  
Klaus-Michael Debatin
Keyword(s):  
Neuroblastoma Cells ◽  
Drug Induced ◽  
Induced Apoptosis

Resistance to TRAIL-induced apoptosis in neuroblastoma cells correlates with a loss of caspase-8 expression

2000 ◽  
Vol 35 (6) ◽  
pp. 603-607 ◽  
Author(s):  
Angelika Eggert ◽  
Michael A. Grotzer ◽  
Tycho J. Zuzak ◽  
Barbara R. Wiewrodt ◽  
Naohiko Ikegaki ◽  
...  
Keyword(s):  
Neuroblastoma Cells ◽  
Caspase 8 ◽  
Induced Apoptosis

scholarly journals Phytic Acid Protects against 6-Hydroxydopamine-Induced Dopaminergic Neuron Apoptosis in Normal and Iron Excess Conditions in a Cell Culture Model

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Qi Xu ◽  
Anumantha G. Kanthasamy ◽  
Manju B. Reddy
Keyword(s):  
Dna Fragmentation ◽  
Phytic Acid ◽  
Caspase 3 ◽  
Fold Increase ◽  
Iron Chelator ◽  
Culture Model ◽  
A Cell ◽  
Induced Apoptosis ◽  
6 Hydroxydopamine ◽  
Stress Dependent

Iron may play an important role in Parkinson's disease (PD) since it can induce oxidative stress-dependent neurodegeneration. The objective of this study was to determine whether the iron chelator, phytic acid (IP6) can protect against 6-hydroxydopamine- (6-OHDA-) induced apoptosis in immortalized rat mesencephalic dopaminergic cells under normal and iron-excess conditions. Caspase-3 activity was increased about 6-fold after 6-OHDA treatment (compared to control; ) and 30 μmol/L IP6 pretreatment decreased it by 38% (). Similarly, a 63% protection () against 6-OHDA induced DNA fragmentation was observed with IP6 pretreatment. Under iron-excess condition, a 6-fold increase in caspase-3 activity () and a 42% increase in DNA fragmentation () with 6-OHDA treatment were decreased by 41% () and 27% (), respectively, with 30 μmol/L IP6. Together, our data suggest that IP6 protects against 6-OHDA-induced cell apoptosis in both normal and iron-excess conditions, and IP6 may offer neuroprotection in PD.

scholarly journals Aqueous Extract of Davallia mariesii Attenuates 6-Hydroxydopamine-Induced Oxidative Damage and Apoptosis in B35 Cells Through Inhibition of Caspase Cascade and Activation of PI3K/AKT/GSK-3β Pathway

Nutrients ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 1449 ◽  
Author(s):  
Chi-Rei Wu ◽  
Hung-Chi Chang ◽  
Yih-Dih Cheng ◽  
Wan-Cheng Lan ◽  
Shu-Er Yang ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Radical Scavenging ◽  
Neuroblastoma Cells ◽  
Antioxidant Defenses ◽  
Protective Mechanism ◽  
Scavenging Capacity ◽  
Intracellular Ros ◽  
Caspase Cascade ◽  
Gsk 3Β ◽  
6 Hydroxydopamine

The medicinal ferns of Polydiaceae and Davalliaceae species are called “Gusuibu” by Chinese physicians and used as antiaging dietary medicines. Our previous report revealed that Drynaria fortunei (Polydiaceae) protected against 6-hydroxydopamine (6-OHDA)-induced oxidative damage via the PI3K/AKT pathway in B35 neuroblastoma cells. The present study compares the antioxidant phytoconstituent contents and radical scavenging capacities of five Davalliaceae species. The further aim was to clarify the protective mechanism of Davallia mariesii (DM) against 6-OHDA-induced oxidative damage and apoptosis in B35 cells. The results show that Araiostegia perdurans (AP) and DM extracts have better radical scavenging capacities against 1,1-diphenyl-2-picryhydrazyl (DPPH) and reactive oxygen species (ROS) than other Davalliaceae species. However, only DM extract inhibited 6-OHDA autoxidation under cell-free systems and increased cell viability, compared to B35 cells solely exposed to 6-OHDA. DM extract decreased apoptosis and restored mitochondrial expression in 6-OHDA-treated B35 cells. Additional data indicated that DM extract decreased intracellular ROS and nitric oxide levels generated by 6-OHDA exposure. DM extract also restored glutathione (GSH) levels and the activities of glutathione peroxidase and reductase, and then decreased the elevated malondialdehyde (MDA) levels. Finally, DM extract regulated the protein expression of the caspase cascade and PI3K/AKT/GSK-3β pathways. These results suggest that the protective mechanism of DM extract against 6-OHDA-induced oxidative damage and apoptosis might be related to its radical scavenging capacity, maintaining the mitochondrial function to inhibit the Bcl-2/caspase cascade pathway and activating intracellular antioxidant defenses (GSH recycling, HO-1 and NQO-1) by modulating the activation of the PI3K/AKT/GSK-3β pathway.

Bax-activation during lidocaine-induced apoptosis in human neuroblastoma cells

2007 ◽  
Vol 32 (Suppl. 1) ◽  
pp. 186
Author(s):  
M. F. Stevens ◽  
S. Braun ◽  
H. Hermanns ◽  
P. Lipfert ◽  
F. Essmann ◽  
...  
Keyword(s):  
Neuroblastoma Cells ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Induced Apoptosis
Sign in / Sign up

Export Citation Format

Share Document