The coupling of acetylcholine-induced BK channel and calcium channel in guinea pig saccular type II vestibular hair cells

2007 ◽  
Vol 1129 ◽  
pp. 110-115 ◽  
Author(s):  
Wei-Jia Kong ◽  
Chang-Kai Guo ◽  
Xiao-Wen Zhang ◽  
Xiong Chen ◽  
Song Zhang ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Calcium Channel ◽  
Hair Cells ◽  
Bk Channel ◽  
Type Ii ◽  
Vestibular Hair Cells

The properties of ACh-induced BK currents in guinea pig type II vestibular hair cells

2005 ◽  
Vol 209 (1-2) ◽  
pp. 1-9 ◽  
Author(s):  
Wei-Jia Kong ◽  
Chang-Kai Guo ◽  
Song Zhang ◽  
Jin Hao ◽  
Yan-jun Wang ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Hair Cells ◽  
Type Ii ◽  
Vestibular Hair Cells

scholarly journals Two Distinct Channels Mediated by m2mAChR and α9nAChR Co-Exist in Type II Vestibular Hair Cells of Guinea Pig

2013 ◽  
Vol 14 (5) ◽  
pp. 8818-8831 ◽  
Author(s):  
Tao Zhou ◽  
Yi Wang ◽  
Chang-Kai Guo ◽  
Wen-Juan Zhang ◽  
Hong Yu ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Hair Cells ◽  
Type Ii ◽  
Vestibular Hair Cells

Molecular characterization of an inward rectifier channel (IKir) found in avian vestibular hair cells: cloning and expression of pKir2.1

2004 ◽  
Vol 19 (2) ◽  
pp. 155-169 ◽  
Author(s):  
Manning J. Correia ◽  
Thomas G. Wood ◽  
Deborah Prusak ◽  
Tianxiang Weng ◽  
Katherine J. Rennie ◽  
...  
Keyword(s):  
Hair Cells ◽  
Cho Cells ◽  
Dwell Time ◽  
Single Channel ◽  
Cloning And Expression ◽  
Single Type ◽  
Type I ◽  
Type Ii ◽  
Vestibular Hair Cells ◽  
Inwardly Rectifying

A fast inwardly rectifying current has been observed in some of the sensory cells (hair cells) of the inner ear of several species. While the current was presumed to be an IKir current, contradictory evidence existed as to whether the cloned channel actually belonged to the Kir2.0 subfamily of potassium inward rectifiers. In this paper, we report for the first time converging evidence from electrophysiological, biochemical, immunohistochemical, and genetic studies that show that the Kir2.1 channel carries the fast inwardly rectifying currents found in pigeon vestibular hair cells. Following cytoplasm extraction from single type II and multiple pigeon vestibular hair cells, mRNA was reverse transcribed, amplified, and sequenced. The open reading frame (ORF), consisting of a 1,284-bp nucleotide sequence, showed 94, 85, and 83% identity with Kir2.1 subunit sequences from chick lens, Kir2 sequences from human heart, and a mouse macrophage cell line, respectively. Phylogenetic analyses revealed that pKir2.1 formed an immediate node with hKir2.1 but not with hKir2.2–2.4. Hair cells (type I and type II) and supporting cells in the sensory epithelium reacted positively with a Kir2.1 antibody. The whole cell current recorded in oocytes and CHO cells, transfected with pigeon hair cell Kir2.1 (pKir2.1), demonstrated blockage by Ba2+ and sensitivity to changing K+ concentration. The mean single-channel linear slope conductance in transfected CHO cells was 29 pS. The open dwell time was long (∼300 ms at −100 mV), and the closed dwell time was short (∼34 ms at −100 mV). Multistates ranging from 3–6 were noted in some single-channel responses. All of the above features have been described for other Kir2.1 channels. Current clamp studies of native pigeon vestibular hair cells illustrated possible physiological roles of the channel and showed that blockage of the channel by Ba2+ depolarized the resting membrane potential by ∼30 mV. Negative currents hyperpolarized the membrane ∼20 mV before block but ∼60 mV following block. RT-PCR studies revealed that the pKir2.1 channels found in pigeon vestibular hair cells were also present in pigeon vestibular nerve, vestibular ganglion, lens, neck muscle, brain (brain stem, cerebellum and optic tectum), liver, and heart.

Temporal Bone Studies of the Human Peripheral Vestibular System

2000 ◽  
Vol 109 (5_suppl) ◽  
pp. 20-25 ◽  
Author(s):  
Kojiro Tsuji ◽  
Steven D. Rauch ◽  
Conrad Wall ◽  
Luis Velázquez-Villaseñor ◽  
Robert J. Glynn ◽  
...  
Keyword(s):  
Hair Cell ◽  
Hair Cells ◽  
Ganglion Cells ◽  
Significant Loss ◽  
Small Sample ◽  
Type I ◽  
Type Ii ◽  
Vestibular Hair Cells ◽  
Scarpa's Ganglion ◽  
Temporal Bones

Quantitative assessments of vestibular hair cells and Scarpa's ganglion cells were performed on 17 temporal bones from 10 individuals who had well-documented clinical evidence of aminoglycoside ototoxicity (streptomycin, kanamycin, and neomycin). Assessment of vestibular hair cells was performed by Nomarski (differential interference contrast) microscopy. Hair cell counts were expressed as densities (number of cells per 0.01 mm2 surface area of the sensory epithelium). The results were compared with age-matched normal data. Streptomycin caused a significant loss of both type I and type II hair cells in all 5 vestibular sense organs. In comparing the ototoxic effect on type I versus type II hair cells, there was greater type I hair cell loss for all 3 cristae, but not for the maculae. The vestibular ototoxic effects of kanamycin appeared to be similar to those of streptomycin, but the small sample size precluded definitive conclusions from being made. Neomycin did not cause loss of vestibular hair cells. Within the limits of this study (maximum postototoxicity survival time of 12 months), there was no significant loss of Scarpa's ganglion cells for any of the 3 drugs. The findings have implications in several clinical areas, including the correlation of vestibular test results to pathological findings, the rehabilitation of patients with vestibular ototoxicity, the use of aminoglycosides to treat Meniere's disease, and the development of a vestibular prosthesis.

Motile responses of isolated guinea pig vestibular hair cells

1991 ◽  
Vol 127 (2) ◽  
pp. 231-236 ◽  
Author(s):  
Jean Valat ◽  
Corinne Griguer ◽  
Jacques Lehouelleur ◽  
Alain Sans
Keyword(s):  
Guinea Pig ◽  
Hair Cells ◽  
Vestibular Hair Cells

Mechanically Induced Calcium Increases in Isolated Vestibular Hair Cells of the Guinea Pig

1995 ◽  
Vol 115 (6) ◽  
pp. 759-764 ◽  
Author(s):  
José A. López-Escámez ◽  
Jochen Schacht
Keyword(s):  
Guinea Pig ◽  
Hair Cells ◽  
Vestibular Hair Cells

M2 muscarinic ACh receptors sensitive BK channels mediate cholinergic inhibition of type II vestibular hair cells

2012 ◽  
Vol 285 (1-2) ◽  
pp. 13-19 ◽  
Author(s):  
Chang-Kai Guo ◽  
Yi Wang ◽  
Tao Zhou ◽  
Hong Yu ◽  
Wen-Juan Zhang ◽  
...  
Keyword(s):  
Hair Cells ◽  
Bk Channels ◽  
Type Ii ◽  
Vestibular Hair Cells ◽  
Ach Receptors
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