scholarly journals Human postmortem tissue: What quality markers matter?

2006 ◽  
Vol 1123 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Ana D. Stan ◽  
Subroto Ghose ◽  
Xue-Min Gao ◽  
Rosalinda C. Roberts ◽  
Kelly Lewis-Amezcua ◽  
...  
Keyword(s):  
Quality Markers ◽  
Human Postmortem Tissue

Simultaneous Determination of Eight Potential Q-Markers in Zishen Tongguan Capsules Based on UHPLC-MS/MS

2020 ◽  
Vol 17 (1) ◽  
pp. 47-56
Author(s):  
Shun Liu ◽  
Xun Wang ◽  
Kaiping Zou ◽  
Wei Liu ◽  
Cunyu Li ◽  
...  
Keyword(s):  
Quality Control ◽  
Chinese Medicine ◽  
Simultaneous Determination ◽  
High Performance ◽  
Multiple Reaction Monitoring ◽  
Gradient Elution ◽  
Quality Markers ◽  
High Repeatability ◽  
Comprehensive Study

Background: Zishen Tongguan (ZSTG) capsules were prepared at the Affiliated Hospital of Nanjing University of Chinese Medicine and have been proven to be clinically effective for treating pyelonephritis and benign prostatic hyperplasia. However, the quality standards are not ideal; a comprehensive study of the “quality markers” (Q-markers), the chemicals inherent in traditional Chinese medicine and its preparations, has not been carried out. Experimental Methods: In this paper, a sensitive and specific ultra-high-performance liquid chromatographictandem mass spectrometry (UHPLC-MS/MS) method was developed for the simultaneous determination of eight potential Q-markers of ZSTG, including timosaponin A3, berberine, jatrorrhizine, phellodendrine, palmatine, mangiferin, neomangiferin, and timosaponin BII. A Kromasil 100-3.5 C18 column was used with a mobile phase of 0.2% formic acid with acetonitrile, and gradient elution at a flow rate of 0.2 mL/min was achieved in 13 minutes and used for separation. Detection was performed in positive/negative mode with multiple reaction monitoring (MRM). Results: The analytical method was validated in terms of the sensitivity, linearity, accuracy, precision, repeatability, stability and recovery. The method established here was successfully applied to study the potential Q-markers in 8 batches of commercial samples, which demonstrated its use in improving the quality control of ZSTG. Conclusion: The developed method had high repeatability and accuracy and was suitable for the simultaneous analysis of multiple Q-markers, which may provide a new basis for the comprehensive assessment and overall quality control of ZSTG.

scholarly journals Difructose anhydrides as quality markers of honey and coffee

2006 ◽  
Vol 39 (7) ◽  
pp. 801-806 ◽  
Author(s):  
A. Montilla ◽  
A.I. Ruiz-Matute ◽  
M.L. Sanz ◽  
I. Martínez-Castro ◽  
M.D. del Castillo
Keyword(s):  
Quality Markers

scholarly journals Goji Berry: Quality Assessment and Crop Adaptation of Plants Cultivated in Tuscany (Italy) by Combination of Carotenoid and DNA Analyses

2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Giada Capecchi ◽  
Emanuele Goti ◽  
Elena Nicolai ◽  
Maria Camilla Bergonzi ◽  
Roberto Monnanni ◽  
...  
Keyword(s):  
Quality Control ◽  
Dna Barcoding ◽  
Analytical Methods ◽  
Hplc Analysis ◽  
European Pharmacopoeia ◽  
Plant Adaptation ◽  
Dna Analyses ◽  
Berry Quality ◽  
Quality Markers ◽  
Crucial Information

In this study HPLC analysis for the evaluation of carotenoids and DNA barcoding are reported for three different samples of Lycium cultivated in Tuscany (Italy). These two analytical methods can represent integrative methods for quality control of goji, giving also crucial information on the plant adaptation to different environments. Hence, carotenoids represent the quality markers proposed by the monograph of the European Pharmacopoeia, while DNA barcoding can differentiate between species and populations and is useful for the detection of the homogeneity of the samples.

Height profile of some air quality markers in the urban atmosphere surrounding a 100m tower building

1998 ◽  
Vol 32 (20) ◽  
pp. 3569-3580 ◽  
Author(s):  
Federico Maria Rubino ◽  
Lucia Floridia ◽  
Manuela Tavazzani ◽  
Silvia Fustinoni ◽  
Rosario Giampiccolo ◽  
...  
Keyword(s):  
Air Quality ◽  
Urban Atmosphere ◽  
Height Profile ◽  
Quality Markers

scholarly journals An Integrated Strategy for Rapid Discovery and Identification of Quality Markers in Gardenia Fructus Using an Omics Discrimination-Grey Correlation-Biological Verification Method

2021 ◽  
Vol 12 ◽  
Author(s):  
Rong Dong ◽  
Qingping Tian ◽  
Yongping Shi ◽  
Shanjun Chen ◽  
Yougang Zhang ◽  
...  
Keyword(s):  
Liver Injury ◽  
Evaluation System ◽  
Multiple Infection ◽  
Active Components ◽  
Grey Correlation ◽  
Liver Protection ◽  
Quality Markers ◽  
Anti Inflammatory ◽  
Highly Correlated ◽  
Tof Ms

Background: Gardenia Fructus (GF), a traditional Chinese medicine of Gardenia Ellis in Rubiaceae family, has the potential to clear heat and purge fire and has been widely used to treat multiple infection-related diseases. However, the quality markers (Q-Markers) of GF have not been revealed comprehensively.Methods: In this experiment, the transgenic zebrafish lines, Tg (l-fabp:EGFP) and Tg (lyz:EGFP), were used to evaluate two main kinds of traditional efficacies of GF, hepatoprotective and anti-inflammatory effects. All the GF samples from different production areas were tested their anti-liver injury and anti-inflammantory activities. High-performance liquid chromatography-quadrupole time-of-flight mass spectrometry method (HPLC-Q-TOF/MS) was employed for herbal metabonomic analysis of GF samples. Gray correlation analysis (GCA) was utilized to screen out the components closely associated with the activities. Finally, the zebrafish model was applied to verify the bioactivity of the crucial components to determine the Q-Markers of GF.Results: The zebrafish models were established by inducing with hydrogen peroxide or copper sulfate and applied to quickly evaluate the hepatoprotective effect and inflammation of GF samples. 27 potentially active components for liver protection and 21 potentially active components with anti-inflammatory properties were identified by herbal metabolomic analysis based on HPLC-Q-TOF/MS. The GCA result showed that five of the 27 components were highly correlated with liver protection, 15 of the 21 components were highly correlated with anti-inflammatory activity. Among them, geniposide and crocin-1 were confirmed their bioactivities on zebrafish experiment to be responsible for the protective effects of GF against liver injury, and genipin-1-β-D-gentiobioside, quinic acid, gardenoside, d-glucuronic acid, l-malic acid, mannitol, rutin, and chlorogenic acid were confirmed to be responsible for the anti-inflammatory effects. Finally, according to the screening principles of Q-Markers, genipin-1-β-D-gentiobioside, geniposide, and gardenoside were preliminarily identified to be the Q-Markers of GF.Conclusion: This study established an effective research strategy of “Omics Discrimination-Grey Correlation-Biological Verification,” which enabled the rapid identification of key pharmacological components of GF. These markers have provided a scientific basis for constructing a modern quality evaluation system for GF.

scholarly journals Identifying Quality Markers of a Safe Surgical Ward: An Interview Study of Patients, Clinical Staff and Administrators

2016 ◽  
Vol 223 (4) ◽  
pp. S109-S110 ◽  
Author(s):  
Yasmin AM. Hassen ◽  
Pritam Singh ◽  
Philip H. Pucher ◽  
Maximilian J. Johnston ◽  
Ara W. Darzi
Keyword(s):  
Interview Study ◽  
Clinical Staff ◽  
Surgical Ward ◽  
Quality Markers

scholarly journals Biochemical changes and physiological quality of corn seeds subjected to different chemical treatments and storage times

2020 ◽  
Vol 42 ◽  
Author(s):  
Thiago Lucas de Oliveira ◽  
Renzo Garcia Von Pinho ◽  
Heloisa Oliveira dos Santos ◽  
Karen Marcelle de Jesus Silva ◽  
Elise de Matos Pereira ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Alcohol Dehydrogenase ◽  
Cold Test ◽  
Chemical Treatments ◽  
Treatment Standard ◽  
Quality Markers ◽  
Physiological Quality ◽  
Storage Times ◽  
And Storage

Abstract: The chemical treatment of corn seeds is widely adopted for protecting crops at the early stages. However, some molecules interfere in the quality of seeds, especially when stored, by accelerating their deterioration. The objective of this work was to investigate the influence of insecticides on the enzymatic expression and quality of corn seeds subjected to storage. Seeds of the hybrids 2B647PW and SHS4070 were subjected to four different chemical treatments (standard treatment, standard + clothianidin, standard + thiamethoxam, and standard + fipronil) and stored for different periods (0, 3, 6, and 9 months). The quality evaluation included the germination test, first germination count, cold test, and health test. The expression of the enzymes α-amylase, esterase, superoxide dismutase, catalase, and alcohol dehydrogenase were determined by the gel electrophoresis technique. The insecticides clothianidin, thiamethoxam, and fipronil reduce the physiological quality of the seeds of hybrid 2B647PW after nine months of storage. The treatment of seeds with the insecticides clothianidin, thiamethoxam, and fipronil reduces the expression of α-amylase, superoxide dismutase, and catalase enzymes after nine months of storage. The enzymes α-amylase, superoxide dismutase, catalase, and alcohol dehydrogenase are good quality markers for hybrid corn seeds.

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