Regeneration of descending projections in Xenopus laevis tadpole spinal cord demonstrated by retrograde double labeling

2006 ◽  
Vol 1088 (1) ◽  
pp. 68-72 ◽  
Author(s):  
Kurt M. Gibbs ◽  
Ben G. Szaro
Keyword(s):  
Spinal Cord ◽  
Xenopus Laevis ◽  
Double Labeling ◽  
Descending Projections ◽  
Xenopus Laevis Tadpole

Calbindin-D28k immunoreactivity in the spinal cord of Xenopus laevis and its participation in ascending and descending projections

2005 ◽  
Vol 66 (4-6) ◽  
pp. 550-554 ◽  
Author(s):  
Ruth Morona ◽  
Nerea Moreno ◽  
Jesús M. López ◽  
Margarita Muñoz ◽  
Hans J. ten Donkelaar ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Xenopus Laevis ◽  
Calbindin D28k ◽  
Descending Projections

A functional scaffold of CNS neurons for the vertebrates: The developing Xenopus laevis spinal cord

2012 ◽  
Vol 72 (4) ◽  
pp. 575-584 ◽  
Author(s):  
Alan Roberts ◽  
Wen-Chang Li ◽  
Stephen R. Soffe
Keyword(s):  
Spinal Cord ◽  
Xenopus Laevis ◽  
Cns Neurons

scholarly journals Descending motor circuitry required for NT-3 mediated locomotor recovery after spinal cord injury in mice

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Qi Han ◽  
Josue D. Ordaz ◽  
Nai-Kui Liu ◽  
Zoe Richardson ◽  
Wei Wu ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Motor Neurons ◽  
Enhanced Recovery ◽  
Neural Pathways ◽  
Locomotor Recovery ◽  
Neurotrophin 3 ◽  
Cord Injury ◽  
Dendritic Atrophy ◽  
Descending Projections

AbstractLocomotor function, mediated by lumbar neural circuitry, is modulated by descending spinal pathways. Spinal cord injury (SCI) interrupts descending projections and denervates lumbar motor neurons (MNs). We previously reported that retrogradely transported neurotrophin-3 (NT-3) to lumbar MNs attenuated SCI-induced lumbar MN dendritic atrophy and enabled functional recovery after a rostral thoracic contusion. Here we functionally dissected the role of descending neural pathways in response to NT-3-mediated recovery after a T9 contusive SCI in mice. We find that residual projections to lumbar MNs are required to produce leg movements after SCI. Next, we show that the spared descending propriospinal pathway, rather than other pathways (including the corticospinal, rubrospinal, serotonergic, and dopaminergic pathways), accounts for NT-3-enhanced recovery. Lastly, we show that NT-3 induced propriospino-MN circuit reorganization after the T9 contusion via promotion of dendritic regrowth rather than prevention of dendritic atrophy.

Descending projections to the rat sacrocaudal spinal cord

1991 ◽  
Vol 307 (1) ◽  
pp. 120-130 ◽  
Author(s):  
Robert L. Masson ◽  
Mary L. Sparkes ◽  
Louis A. Ritz
Keyword(s):  
Spinal Cord ◽  
Descending Projections

scholarly journals Jisuikang Promotes the Repair of Spinal Cord Injury in Rats by Regulating NgR/RhoA/ROCK Signal Pathway

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Chengjie Wu ◽  
Yuxin Zhou ◽  
Pengcheng Tu ◽  
Guanglu Yang ◽  
Suyang Zheng ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Nerve Regeneration ◽  
Motor Function ◽  
Signal Pathway ◽  
Nerve Tissue ◽  
Inclined Plate ◽  
Double Labeling ◽  
Cord Injury ◽  
Potential Applicability

Jisuikang (JSK) is an herbal formula composed of many kinds of traditional Chinese medicine, which has been proved to be effective in promoting the rehabilitation of patients with spinal cord injury (SCI) after more than ten years of clinical application. However, the mechanisms of JSK promoting nerve regeneration are yet to be clarified. The aim of this study was to investigate the effects of JSK protecting neurons, specifically the regulation of NgR/RhoA/ROCK signal pathway. The motor function of rats was evaluated by the BBB score and inclined plate test, Golgi staining and transmission electron microscope were used to observe the microstructure of nerve tissue, and fluorescence double-labeling method was used to detect neuronal apoptosis. In this study, we found that JSK could improve the motor function of rats with SCI, protect the microstructure (mitochondria, endoplasmic reticulum, and dendritic spine) of neurons, and reduce the apoptosis rate of neurons in rats with SCI. In addition, JSK could inhibit the expression of Nogo receptor (NgR) in neurons and the NgR/RhoA/ROCK signal pathway in rats with SCI. These results indicated JSK could improve the motor function of rats with SCI by inhibiting the NgR/RhoA/ROCK signal pathway, which suggests the potential applicability of JSK as a nerve regeneration agent.

scholarly journals Expression of the Ca2+-binding protein, parvalbumin, during embryonic development of the frog, Xenopus laevis

1987 ◽  
Vol 104 (4) ◽  
pp. 841-847 ◽  
Author(s):  
BK Kay ◽  
AJ Shah ◽  
WE Halstead
Keyword(s):  
Xenopus Laevis ◽  
Fusion Protein ◽  
Binding Protein ◽  
Expression Library ◽  
Simple Method ◽  
High Affinity ◽  
Tadpole Tail ◽  
Developing Muscle ◽  
Beta Galactosidase ◽  
Xenopus Laevis Tadpole

A cDNA segment encoding the Ca2+-binding protein, parvalbumin, was isolated with the use of antibodies, from a lambda gtll expression library of Xenopus laevis tadpole poly(A)+ RNAs. The bacterially expressed beta-galactosidase-parvalbumin fusion protein of one lambda recombinant shows high affinity 45Ca2+ binding. The sequence of the tadpole parvalbumin is highly similar to previously characterized beta-parvalbumins of other organisms. Data from protein and RNA blotting experiments demonstrate that parvalbumin is absent in oocytes, eggs, and early staged embryos, and only becomes expressed during embryogenesis at the time of myogenesis. The protein can be detected in individual developing muscle cells and in muscle fibers of tadpole tail muscles. A simple method is also described for the isolation of neural tube-notochord-somite complexes from Xenopus embryos.

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