In vivo and in vitro effects of proline on some parameters of oxidative stress in rat brain

2003 ◽  
Vol 991 (1-2) ◽  
pp. 180-186 ◽  
Author(s):  
Daniela Delwing ◽  
Caren Serra Bavaresco ◽  
Fábria Chiarani ◽  
Clóvis Milton Duval Wannmacher ◽  
Moacir Wajner ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rat Brain ◽  
In Vitro Effects

Lipoic acid prevents oxidative stress in vitro and in vivo by an acute hyperphenylalaninemia chemically-induced in rat brain

2010 ◽  
Vol 292 (1-2) ◽  
pp. 89-95 ◽  
Author(s):  
Tarsila Barros Moraes ◽  
Fernanda Zanin ◽  
Andrea da Rosa ◽  
Amanda de Oliveira ◽  
Juliana Coelho ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rat Brain ◽  
Lipoic Acid ◽  
Chemically Induced

scholarly journals Responsiveness of Lycopersicon pimpinellifolium to acute UV-C exposure: histo-cytochemistry of the injury and DNA damage.

2007 ◽  
Vol 54 (2) ◽  
pp. 273-280 ◽  
Author(s):  
M Iriti ◽  
S Guarnieri ◽  
F Faoro
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Positive Control ◽  
Lycopersicon Pimpinellifolium ◽  
Irradiation Cell ◽  
Leaf Tissues ◽  
In Vitro Effects ◽  
Uv C

The in vivo and in vitro effects of UV-C (254 nm) exposure (0.039 watt . m(-2) . s for 2 h) of currant tomato (Lycopersicon pimpinellifolium), indigenous to Peru and Ecuador, were assayed. H(2)O(2) deposits, dead cells and DNA damage were localized, 12/24 h after irradiation, mainly in periveinal parenchyma of the 1st and 2nd order veins of the leaves, and before the appearance of visible symptoms, which occurred 48 h after irradiation. Cell death index was of 43.5 +/- 12% in exposed leaf tissues, 24 h after treatment. In currant tomato protoplasts, the percentage of viable cells dropped 1 h after UV-C irradiation from 97.42 +/- 2.1% to 43.38 +/- 4.2%. Afterwards, the protoplast viability progressively decreased to 40.16 +/- 7.25% at 2 h, to 38.31 +/- 6.9% at 4 h, and to 36.46 +/- 1.84% at 6 h after the exposure. The genotoxic impact of UV-C radiation on protoplasts was assessed with single cell gel electrophoresis (SCGE, or comet assay). UV-C treatment greatly enhanced DNA migration, with 75.37 +/- 3.7% of DNA in the tail versus 7.88 +/- 5.5% in the case of untreated nuclei. Oxidative stress by H(2)O(2) used as a positive control, induced a similar damage on non-irradiated protoplasts, with 71.59 +/- 5.5% of DNA in the tail, whereas oxidative stress imposed on UV-C irradiated protoplasts slightly increased the DNA damage (85.13 +/- 4.1%). According to these results, SCGE of protoplasts could be an alternative to nuclei extraction directly from leaf tissues.

scholarly journals Protective role of maize purple plant pigment against oxidative stress in fluorosis rat brain

2020 ◽  
Vol 11 (1) ◽  
pp. 89-95
Author(s):  
Boyan Li ◽  
Keyana Nozzari Varkani ◽  
Lu Sun ◽  
Bo Zhou ◽  
Xiaohong Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Rat Brain ◽  
Neuronal Apoptosis ◽  
Protective Role ◽  
Fluoride Toxicity ◽  
Plant Pigment

AbstractIn fluorosis-endemic areas, exposure to high levels of fluoride causes neurotoxicity such as lowered intelligence and cognitive impairment. Oxidative damage is critical to pathophysiologic processes of fluoride intoxication, and neurotoxicity of fluoride may be associated with oxidative stress. In previous studies, maize purple plant pigment (MPPP), which was rich in anthocyanins, showed a strong scavenging activity in vitro and in vivo. The present study aimed to determine whether treatment with MPPP can alleviate fluoride-induced oxidative damage in rat brain. After 3 months of experiment, brain tissues were assayed for oxidative stress variables, histological and Western blotting examinations. Our results showed that MPPP reduced the elevated malondialdehyde levels, increased superoxide dismutase activity, and further attenuated histopathological alterations and mitigated neuronal apoptosis. Importantly, MPPP also reversed changes in Bax and Bcl-2. Therefore, it was speculated that MPPP protects brain tissue from fluoride toxicity through its antioxidant capacity.

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