Fourier transform infrared microspectroscopy reveals biochemical changes associated with glioma stem cell differentiation

2015 ◽  
Vol 207 ◽  
pp. 90-96 ◽  
Author(s):  
Saša Kenig ◽  
Diana E. Bedolla ◽  
Giovanni Birarda ◽  
Valentina Faoro ◽  
Elisa Mitri ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Fourier Transform Infrared ◽  
Stem Cell Differentiation ◽  
Biochemical Changes ◽  
Glioma Stem Cell ◽  
Infrared Microspectroscopy ◽  
Fourier Transform Infrared Microspectroscopy

scholarly journals Fourier-Transform Infrared Microspectroscopy (FT-IR) Study on Caput and Cauda Mouse Spermatozoa

Proceedings ◽  
2019 ◽  
Vol 42 (1) ◽  
pp. 19 ◽  
Author(s):  
Marianna Portaccio ◽  
Sonia Errico ◽  
Teresa Chioccarelli ◽  
Gilda Cobellis ◽  
Maria Lepore
Keyword(s):  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Biochemical Changes ◽  
Infrared Microspectroscopy ◽  
Fourier Transform Infrared Microspectroscopy ◽  
In Vitro Investigation ◽  
Ft Ir ◽  
Protein Nucleic Acid ◽  
Ir Study

Fourier-Transform Infrared micro-spectroscopy (µFT-IR) was used for an in vitro investigation on spermatozoa (SPZ) samples separately collected from caput and cauda of mouse epididymis. SPZ are characterized by deep biochemical changes during the transit along the epididymis and they can constitute ideal candidates for a µFT-IR investigation, thanks to the ability of this technique in analyzing cells at a molecular level. Appreciable differences were reported in the infrared spectra from caput and cauda SPZ, and biochemical changes in protein, nucleic acid, lipid, and carbohydrate content of cells were evidenced. The present investigation indicates that µFT-IR can constitute a valuable tool for monitoring, in an easy and fast way, the changes suffered by SPZ during the transit along the epididymis.

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