Interaction of the adenoviral IVa2 protein with a truncated viral DNA packaging sequence

2009 ◽  
Vol 140 (1-3) ◽  
pp. 78-90 ◽  
Author(s):  
Teng-Chieh Yang ◽  
Qin Yang ◽  
Nasib Karl Maluf
Keyword(s):  
Dna Packaging ◽  
Viral Dna ◽  
Viral Dna Packaging ◽  
Packaging Sequence

scholarly journals Role for the Adenovirus IVa2 Protein in Packaging of Viral DNA

2001 ◽  
Vol 75 (21) ◽  
pp. 10446-10454 ◽  
Author(s):  
Wei Zhang ◽  
Jonathan A. Low ◽  
Joan B. Christensen ◽  
Michael J. Imperiale
Keyword(s):  
Gene Transfer ◽  
Direct Evidence ◽  
Helper Virus ◽  
Dna Packaging ◽  
Chimeric Virus ◽  
Viral Dna ◽  
293 Cells ◽  
Inverted Terminal Repeats ◽  
Viral Dna Packaging ◽  
Packaging Sequence

ABSTRACT Although it has been demonstrated that the adenovirus IVa2 protein binds to the packaging domains on the viral chromosome and interacts with the viral L1 52/55-kDa protein, which is required for viral DNA packaging, there has been no direct evidence demonstrating that the IVa2 protein is involved in DNA packaging. To understand in greater detail the DNA packaging mechanisms of adenovirus, we have asked whether DNA packaging is serotype or subgroup specific. We found that Ad7 (subgroup B), Ad12 (subgroup A), and Ad17 (subgroup D) cannot complement the defect of an Ad5 (subgroup C) mutant,pm8001, which does not package its DNA due to a mutation in the L1 52/55-kDa gene. This indicates that the DNA packaging systems of different serotypes cannot interact productively with Ad5 DNA. Based on this, a chimeric virus containing the Ad7 genome except for the inverted terminal repeats and packaging sequence from Ad5 was constructed. This chimeric virus replicates its DNA and synthesizes Ad7 proteins, but it cannot package its DNA in 293 cells or 293 cells expressing the Ad5 L1 52/55-kDa protein. However, this chimeric virus packages its DNA in 293 cells expressing the Ad5 IVa2 protein. These results indicate that the IVa2 protein plays a role in viral DNA packaging and that its function is serotype specific. Since this chimeric virus cannot package its own DNA, but produces all the components for packaging Ad7 DNA, it may be a more suitable helper virus for the growth of Ad7 gutted vectors for gene transfer.

Translation of the long-term fundamental studies on viral DNA packaging motors into nanotechnology and nanomedicine

2020 ◽  
Vol 63 (8) ◽  
pp. 1103-1129 ◽  
Author(s):  
Chenxi Liang ◽  
Tao Weitao ◽  
Lixia Zhou ◽  
Peixuan Guo
Keyword(s):  
Dna Packaging ◽  
Viral Dna ◽  
Viral Dna Packaging

A Minimal Kinetic Model for a Viral DNA Packaging Machine†

Biochemistry ◽  
2004 ◽  
Vol 43 (2) ◽  
pp. 289-299 ◽  
Author(s):  
Qin Yang ◽  
Carlos Enrique Catalano
Keyword(s):  
Kinetic Model ◽  
Dna Packaging ◽  
Viral Dna ◽  
Packaging Machine ◽  
Viral Dna Packaging

scholarly journals A Mechanochemical Model of a Viral DNA Packaging Motor

2010 ◽  
Vol 98 (3) ◽  
pp. 656a
Author(s):  
Jin Yu ◽  
Jeffrey Moffitt ◽  
Craig Hetherington ◽  
Carlos Bustamante ◽  
George Oster
Keyword(s):  
Dna Packaging ◽  
Viral Dna ◽  
Dna Packaging Motor ◽  
Viral Dna Packaging ◽  
Mechanochemical Model ◽  
Viral Dna Packaging Motor

Mechanochemistry of a Viral DNA Packaging Motor

2010 ◽  
Vol 400 (2) ◽  
pp. 186-203 ◽  
Author(s):  
Jin Yu ◽  
Jeffrey Moffitt ◽  
Craig L. Hetherington ◽  
Carlos Bustamante ◽  
George Oster
Keyword(s):  
Dna Packaging ◽  
Viral Dna ◽  
Dna Packaging Motor ◽  
Viral Dna Packaging ◽  
Viral Dna Packaging Motor
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