Towards the mechanism of trimeric purine nucleoside phosphorylases: Stopped-flow studies of binding of multisubstrate analogue inhibitor — 2-amino-9-[2-(phosphonomethoxy)ethyl]-6-sulfanylpurine

2007 ◽  
Vol 125 (2-3) ◽  
pp. 260-268 ◽  
Author(s):  
B. Wielgus-Kutrowska ◽  
J.M. Antosiewicz ◽  
M. Dƚugosz ◽  
A. Holý ◽  
A. Bzowska
Keyword(s):  
Purine Nucleoside ◽  
Stopped Flow ◽  
Nucleoside Phosphorylases

Interactions of Trimeric Purine Nucleoside Phosphorylases with Ground State Analogues — Calorimetric and Fluorimetric Studies

ChemInform ◽  
2004 ◽  
Vol 35 (9) ◽  
Author(s):  
Beata Wielgus-Kutrowska ◽  
Joachim Frank ◽  
Antonin Holy ◽  
Gertraud Koellner ◽  
Agnieszka Bzowska
Keyword(s):  
Ground State ◽  
Purine Nucleoside ◽  
Nucleoside Phosphorylases

Localization of purine and pyrimidine nucleoside phosphorylases in heart, kidney, and liver

1980 ◽  
Vol 239 (6) ◽  
pp. H721-H730 ◽  
Author(s):  
R. Rubio ◽  
R. M. Berne
Keyword(s):  
Guinea Pig ◽  
Kupffer Cells ◽  
Cellular Localization ◽  
Purine Nucleoside Phosphorylase ◽  
Degradation Products ◽  
Purine Nucleoside ◽  
Capillary Endothelium ◽  
Cell Fractionation ◽  
Nucleoside Phosphorylase ◽  
Nucleoside Phosphorylases

In isolated livers and kidneys perfused with Krebs-Henseleit solution, the relationship of the concentration of adenosine (Ado) to that of its degradation products inosine (Ino) and hypoxanthine (Hyp) in biliary, urinary, and venous effluents were determined. They revealed ratios of Hyp:Ado:Ino, 1.9:1:0.9, 0.7:1:0.6, and 1.3:1:0.5 for guinea pig biliary, guinea pig urinary, and rat urinary effluents, respectively, and their respective venous effluent were 58:1:29, 8.6:1:5.4, and 7.4:1:3.2. The greater proportion of Ino and Hyp in the venous effluents suggests active production in Ino and Hyp at the vessel wall. Purine nucleoside phosphorylase localization was determined histochemically and found most active in the cytoplasm of capillary endothelium and Kupffer cells. Thus, there is agreement between purine analysis and histochemical findings. The reliability of the histochemical technique was also tested by comparing activities of purine nucleoside phosphorylase (a cytoplasmic enzyme) and pyrmidine nucleoside phosphorylase (a nuclear enzyme) that catalyze similar reactions (nucleoside + inorganic phosphate in equilibrium base + ribose-1-phosphate) but with different base specificites and cellular localization, as indicated by cell fractionation studies. The histochemical results show that in contrast to the purine nucleoside phosphorylase, the pyrmidine specific enzyme was most active in the nuclei of endothelial and Kupffer cells. Thus, the technique discriminates between the two enzymes.

6-Methylpurine derived sugar modified nucleosides: Synthesis and evaluation of their substrate activity with purine nucleoside phosphorylases

2016 ◽  
Vol 65 ◽  
pp. 9-16 ◽  
Author(s):  
Abdalla E.A. Hassan ◽  
Reham A.I. Abou-Elkhair ◽  
William B. Parker ◽  
Paula W. Allan ◽  
John A. Secrist
Keyword(s):  
Modified Nucleosides ◽  
Purine Nucleoside ◽  
Nucleoside Phosphorylases

scholarly journals Purine nucleoside phosphorylases from hyperthermophilic Archaea require a CXC motif for stability and folding

FEBS Journal ◽  
2009 ◽  
Vol 276 (20) ◽  
pp. 5799-5805 ◽  
Author(s):  
Giovanna Cacciapuoti ◽  
Iolanda Peluso ◽  
Francesca Fuccio ◽  
Marina Porcelli
Keyword(s):  
Purine Nucleoside ◽  
Hyperthermophilic Archaea ◽  
Nucleoside Phosphorylases

scholarly journals Substrate specificity of uridine and purine nucleoside phosphorylases of the Escherichia coli whole cells

1988 ◽  
Vol 4 (6) ◽  
pp. 298-302
Author(s):  
A. I. Zintchenko ◽  
L. A. Eroshevskaya ◽  
V. N. Barai ◽  
I. A. Mikhailopulo
Keyword(s):  
Escherichia Coli ◽  
Substrate Specificity ◽  
Purine Nucleoside ◽  
Whole Cells ◽  
Nucleoside Phosphorylases

scholarly journals Transition State Analogue Discrimination by Related Purine Nucleoside Phosphorylases

2006 ◽  
Vol 128 (22) ◽  
pp. 7126-7127 ◽  
Author(s):  
Erika A. Taylor Ringia ◽  
Peter C. Tyler ◽  
Gary B. Evans ◽  
Richard H. Furneaux ◽  
Andrew S. Murkin ◽  
...  
Keyword(s):  
Transition State ◽  
Purine Nucleoside ◽  
Transition State Analogue ◽  
Nucleoside Phosphorylases
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