Dimethyl-pepep: a DNA probe in two-photon excitation cellular imaging

2005 ◽  
Vol 114 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Alessandro Abbotto ◽  
Giancarlo Baldini ◽  
Luca Beverina ◽  
Giuseppe Chirico ◽  
Maddalena Collini ◽  
...  
Keyword(s):  
Cellular Imaging ◽  
Dna Probe ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Energy Transfer in Aminonaphthalimide-Boron-Dipyrromethene (BODIPY) Dyads upon One- and Two-Photon Excitation: Applications for Cellular Imaging

2013 ◽  
Vol 9 (3) ◽  
pp. 797-804 ◽  
Author(s):  
Daniel Collado ◽  
Patricia Remón ◽  
Yolanda Vida ◽  
Francisco Najera ◽  
Pratik Sen ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Cellular Imaging ◽  
Two Photon ◽  
Photon Excitation ◽  
Boron Dipyrromethene ◽  
Two Photon Excitation

scholarly journals Corrigendum: Combined Two-Photon Excitation and d→f Energy Transfer in a Water-Soluble IrIII/EuIIIDyad: Two Luminescence Components from One Molecule for Cellular Imaging

2014 ◽  
Vol 20 (52) ◽  
pp. 17242-17242 ◽  
Author(s):  
Elizabeth Baggaley ◽  
Deng-Ke Cao ◽  
Daniel Sykes ◽  
Stanley W. Botchway ◽  
Julia A. Weinstein ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Cellular Imaging ◽  
Water Soluble ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

New two-photon excitation chromophores for cellular imaging

2003 ◽  
Author(s):  
Laura D'Alfonso ◽  
Giuseppe Chirico ◽  
Maddalena Collini ◽  
Giancarlo Baldini ◽  
Alberto Diaspro ◽  
...  
Keyword(s):  
Cellular Imaging ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

New Two-Photon Excitation Chromophores For Cellular Imaging.

2003 ◽  
Author(s):  
Laura D'alfonso ◽  
Giuseppe Chirico ◽  
Maddalena Collini ◽  
Giancarlo Baldini ◽  
Alberto Diaspro ◽  
...  
Keyword(s):  
Cellular Imaging ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Two-photon excitation microscopy in cellular biophysics

1995 ◽  
Vol 53 ◽  
pp. 62-63
Author(s):  
David W. Piston ◽  
Brian D. Bennett ◽  
Robert G. Summers
Keyword(s):  
Confocal Microscopy ◽  
Laser Beam ◽  
Duty Cycle ◽  
Excited Electronic State ◽  
Input Power ◽  
Two Photon ◽  
Simultaneous Absorption ◽  
Photon Excitation ◽  
Very High ◽  
Two Photon Excitation

Two-photon excitation microscopy (TPEM) provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging and photochemistry. Two-photon excitation arises from the simultaneous absorption of two photons in a single quantitized event whose probability is proportional to the square of the instantaneous intensity. For example, two red photons can cause the transition to an excited electronic state normally reached by absorption in the ultraviolet. In practice, two-photon excitation is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10-5 maintains the average input power on the order of 10 mW, only slightly greater than the power normally used in confocal microscopy.

Two-photon excitation fluorescence microscopy in living systems

1993 ◽  
Vol 51 ◽  
pp. 154-155 ◽  
Author(s):  
David W. Piston
Keyword(s):  
Confocal Microscopy ◽  
Fluorescence Microscopy ◽  
Singlet State ◽  
Excited Electronic State ◽  
Input Power ◽  
Two Photon ◽  
Simultaneous Absorption ◽  
Photon Excitation ◽  
Very High ◽  
Two Photon Excitation

Two-photon excitation fluorescence microscopy provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging. Two-photon excitation arises from the simultaneous absorption of two photons in a single quantitized event whose probability is proportional to the square of the instantaneous intensity. For example, two red photons can cause the transition to an excited electronic state normally reached by absorption in the ultraviolet. In our fluorescence experiments, the final excited state is the same singlet state that is populated during a conventional fluorescence experiment. Thus, the fluorophore exhibits the same emission properties (e.g. wavelength shifts, environmental sensitivity) used in typical biological microscopy studies. In practice, two-photon excitation is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10−5 maintains the average input power on the order of 10 mW, only slightly greater than the power normally used in confocal microscopy.

Two‐photon excitation fluorescence microscopy using a semiconductor laser

Bioimaging ◽  
1995 ◽  
Vol 3 (2) ◽  
pp. 70-75 ◽  
Author(s):  
Pekka E Hänninen ◽  
Martin Schrader ◽  
Erkki Soini ◽  
Stefan W Hell
Keyword(s):  
Fluorescence Microscopy ◽  
Semiconductor Laser ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Two-Photon Excitation Imaging of Glucose Metabolism in Living Tissue

1997 ◽  
Vol 3 (S2) ◽  
pp. 305-306
Author(s):  
David W. Piston
Keyword(s):  
Confocal Microscopy ◽  
Collection Efficiency ◽  
Three Dimensional ◽  
Spatial Filter ◽  
Input Power ◽  
Photon Absorption ◽  
Focal Volume ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Two-photon excitation microscopy (TPEM) provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging and photochemistry. It provides three-dimensional resolution and eliminates background equivalent to an ideal confocal microscope without requiring a confocal spatial filter, whose absence enhances fluorescence collection efficiency. This results in inherent submicron optical sectioning by excitation alone. In practice, TPEM is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10−5 limits the average input power to less than 10 mW, only slightly greater than the power normally used in confocal microscopy. Because of the intensity-squared dependence of the two-photon absorption, the excitation is limited to the focal volume.

Sign in / Sign up

Export Citation Format

Share Document